Citation: Xiao Deng, Shilin Cao, Ning Li, Hong Wu, Thomas J. Smith, Minhua Zong, Wenyong Lou. A magnetic biocatalyst based on mussel-inspired polydopamine and its acylation of dihydromyricetin[J]. Chinese Journal of Catalysis, ;2016, 37(4): 584-595. doi: 10.1016/S1872-2067(15)61045-2 shu

A magnetic biocatalyst based on mussel-inspired polydopamine and its acylation of dihydromyricetin

Xiao Deng ^a,c ,
Shilin Cao ^b,c ,
Ning Li ^c ,
Hong Wu ^c ,
Thomas J. Smith ^d,, ,
Minhua Zong ^a,b ,
Wenyong Lou ^a,c,,

a.
a State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China;
b.
b School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China;
c.
c Laboratory of Applied Biocatalysis, School of Food Science and Technology, South China University of Technology, Guangzhou 510640, Guangdong, China;
d.
d Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, S1 1WB, UK

Corresponding author: Thomas J. Smith, Wenyong Lou,
Received Date: 1 November 2015
Available Online: 4 January 2016
Fund Project: 广东省自然科学基金(S2013020013049) (S2013020013049) 制浆造纸工程国家重点实验室项目(2015C04). (2015C04)

A support made of mussel-inspired polydopamine-coated magnetic iron oxide nanoparticles (PD-MNPs) was prepared and characterized. The widely used Aspergillus niger lipase (ANL) was immobilized on the PD-MNPs (ANL@PD-MNPs) with a protein loading of 138 mg/g and an activity recovery of 83.6% under optimized conditions. For the immobilization, the pH and immobilization time were investigated. The pH and thermal and storage stability of the ANL@PD-MNPs significantly surpassed those of free ANL. The ANL@PD-MNPs had better solvent tolerance than free ANL. The secondary structure of free ANL and ANL@PD-MNPs was analyzed by infrared spectroscopy. A kinetic study demonstrated that the ANL@PD-MNPs had enhanced enzyme-substrate affinity and high catalytic efficiency. The ANL@PD-MNPs was applied as a biocatalyst for the regioselective acylation of dihydromyricetin (DMY) in DMSO and gave a conversion of 79.3%, which was higher than that of previous reports. The ANL@PD-MNPs retained over 55% of its initial activity after 10 cycles of reuse. The ANL@PD-MNPs were readily separated from the reaction system by a magnet. The PD-MNPs is an excellent support for ANL and the resulting ANL@PD-MNPs displayed good potential for the efficient synthesis of dihydromyricetin-3-acetate by enzymatic regioselective acylation.
- Magnetic iron oxide,
- Nanoparticle,
- Polydopamine,
- Aspergillus niger lipase,
- Dihydromyricetin,
- Acylation
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