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Citation: Elsa Cherian, Mahendradas Dharmendirakumar, Gurunathan Baskar. Immobilization of cellulase onto MnO₂ nanoparticles for bioethanol production by enhanced hydrolysis of agricultural waste[J]. Chinese Journal of Catalysis, ;2015, 36(8): 1223-1229. doi: 10.1016/S1872-2067(15)60906-8 shu

Immobilization of cellulase onto MnO₂ nanoparticles for bioethanol production by enhanced hydrolysis of agricultural waste

1.
a Department of Biotechnology, St. Joseph's College of Engineering, Chennai-600119, India;

Corresponding author: Mahendradas Dharmendirakumar,
Received Date: 19 February 2015
Available Online: 16 May 2015

Cellulase is an efficient enzymatic catalyst that hydrolyses cellulosic substances. The high costs associated with using enzymes for industrial applications can be reduced by immobilizing the cellulase. In the current study, cellulase produced by Aspergillus fumigatus JCF was immobilized onto MnO₂ nanoparticles, which improve the activity of cellulase and offer a superior support. The surface characteristics of synthesized MnO₂ nanoparticles and cellulase-bound MnO₂ nanoparticles were investigated by scanning electron microscopy, and Fourier transform infrared spectroscopy was used to analyze the functional characteristics of the immobilized cellulase. The maximum cellulase binding efficiency was 75%. The properties of the immobilized cellulase, including activity, operational pH, temperature, thermal stability, and reusability were investigated and were found to be more stable than for the free enzyme. It was found that cellulase immobilized on MnO₂ nanoparticles could be used to hydrolyze cellulosic substances over a broad range of temperature and pH. The results confirmed that cellulase immobilized on MnO₂ nanoparticles was very efficient in terms of cellulolytic activity.
- Cellulase,
- Immobilization,
- Manganese dioxide,
- Nanobiocatalyst,
- Agricultural waste,
- Hydrolysis,
- Bioethanol
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Immobilization of cellulase onto MnO2 nanoparticles for bioethanol production by enhanced hydrolysis of agricultural waste

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通讯作者: 陈斌, bchen63@163.com

Immobilization of cellulase onto MnO₂ nanoparticles for bioethanol production by enhanced hydrolysis of agricultural waste