Citation: Xianfeng Fu, Jianyong Zheng, Xiangxian Ying, Hongde Yan, Zhao Wang. Investigation of Lipozyme TL IM-catalyzed transesterification using ultraviolet spectrophotometric assay[J]. Chinese Journal of Catalysis, ;2014, 35(4): 553-559. doi: 10.1016/S1872-2067(14)60053-X shu

Investigation of Lipozyme TL IM-catalyzed transesterification using ultraviolet spectrophotometric assay

1.
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, Zhejiang, China

Corresponding author: Zhao Wang,
Received Date: 15 November 2013
Available Online: 7 January 2014
Fund Project: 国家高技术研究发展计划(863计划, 2011AA02A210). (863计划, 2011AA02A210)

A novel ultraviolet (UV) spectrophotometric method for evaluating lipase transesterification activity in organic solvents is described. The model reaction was lipase-catalyzed transesterification between p-nitrophenyl palmitate (pNPP) and n-butanol in n-hexane at 30 ℃. The conversion of pNPP to p-nitrophenol was monitored by measuring UV absorbance changes at 310 nm. Seven commercial lipases were tested using this method, and the results were similar to those obtained using gas chromatography. The UV spectrophotometric method was used to investigate the kinetics of the Lipozyme TL IM-catalyzed reaction of pNPP, and the correlation between synthetic activity and biocatalyst concentration was determined. The method was also used to investigate the Lipozyme TL IM-catalyzed transesterification properties, namely solvent effect, acyl acceptor specificity, alcohol tolerance, optimum reaction temperature, and thermostability.
- Lipase,
- Lipozyme TL IM,
- Transesterification activity,
- Ultraviolet spectrophotometric assay,
- p-Nitrophenyl palmitate,
- Organic solvent
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