Citation: Yun-zhu Wu, Zhi-huang Zhang, Xin Han, Jian Zhang, Wen-ming Zhang, Jun Yin. Affinity Switching for Lysozyme and Dual-responsive Microgels by Stopped-flow Technique:Kinetic Control and Activity Evaluation[J]. Chinese Journal of Polymer Science, ;2017, 35(8): 950-960. doi: 10.1007/s10118-017-1948-z shu

Affinity Switching for Lysozyme and Dual-responsive Microgels by Stopped-flow Technique:Kinetic Control and Activity Evaluation

Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, Hefei University of Technology and Anhui Key Laboratory of Advanced Functional Materials and Devices, Hefei 230009, China

Corresponding author: Jun Yin, yinjun@hfut.edu.cn

^†

Received Date: 9 February 2017
Revised Date: 22 February 2017
Accepted Date: 7 March 2017

Fund Project: the National Natural Science Foundation of China 51673058

The use of proteins as therapeutics in nanomedicine is an emerging research field and has developed rapidly. However, proteins are always vulnerable to renal excretion or digestion by the proteolytic system in vivo, which limits their usage to a large extent. Although biocompatible polymers have been covalently linked to proteins to protect them from recognition by the immune system and prolong their circulation time, the biological activity of them is sometimes decreased. To fill this gap, physical isolation, wrapping, or encapsulation techniques are employed. Up to now, various mature examples were reported, but the whole time scales for guest molecules loading and releasing, especially the initial rapid loading process, were rarely mentioned. Herein, a series of dual-responsive poly(N-isopropylacrylamide-co-methacrylic acid) (P(NIPAM-co-MAA)) microgels were synthesized and employed to investigate the kinetics of in situ complexation and release of lysozyme under external stimuli modulation upon a stopped-flow apparatus, which was suitable for rapid dynamic monitoring. Close inspection of the adsorption kinetics during the early stages (<50 s) revealed that the initial microgel collapse occurred within~1 s, with more rapid transitions being observed when higher lysozyme concentrations were targeted. All the dynamic traces could be well fitted with a double exponential function, suggesting a fast (τ₁) and a slow (τ₂) relaxation time, respectively. Then, the kinetics of releasing bound lysozyme from microgels was carried on by utilizing the pH-responsive property, and the evaluation of the activity of released lysozyme was synchronously measured in a Micrococcus lysodeikticus (M. lysodeikticus) cell suspension. The corresponding relaxation time (τ) was also calculated by fitting the recorded dynamic traces. We speculate that this work can provide basic dynamics data and theoretical basis for microgels based nanocarriers to be used for protein delivery, controlled release, and possible chemical separation.
- Microgels,
- Stimuli-responsive,
- Lysozyme,
- Adsorption,
- Kinetics
1. [1]
  Oya, T., Enoki, T., Grosberg, A.Y., Masamune, S., Sakiyama, T., Takeoka, Y., Tanaka, K., Wang, G.Q., Yilmaz, Y., Feld, M.S., Dasari, R. and Tanaka, T., Science, 1999, 286:1543 doi: 10.1126/science.286.5444.1543
2. [2]
  Miyata, K., Kakizawa, Y., Nishiyama, N., Harada, A., Yamasaki, Y., Koyama, H. and Kataoka, K., J. Am. Chem. Soc., 2004, 126:2355 doi: 10.1021/ja0379666
3. [3]
  Yoshimatsu, K., Lesel, B.K., Yonamine, Y., Beierle, J.M., Hoshino, Y. and Shea, K.J., Angew. Chem. Int. Ed., 2012, 124:2455 doi: 10.1002/ange.v124.10
4. [4]
  Ge, Z.S. and Liu, S.Y., Chem. Soc. Rev., 2013, 42:7289 doi: 10.1039/c3cs60048c
5. [5]
  Deng, Z.Y., Qian, Y.F., Yu, Y.Q., Liu, G.H., Hu, J.M., Zhang, G.Y. and Liu, S.Y., J. Am. Chem. Soc., 2016, 138:10452 doi: 10.1021/jacs.6b04115
6. [6]
  Li, Z.B., Xiang, Y.H., Zhou, X.J., Nie, J.J., Peng, M. and Du, B.Y., Chinese J. Polym. Sci., 2015, 33(11):1516 doi: 10.1007/s10118-015-1694-z
7. [7]
  Liu, J.J., Yan, Y.F. and Yao, P., Chinese J. Polym. Sci., 2011, 29(4):397 doi: 10.1007/s10118-011-1054-6
8. [8]
  Xing, S.Y., Guan, Y. and Zhang, Y.J., Macromolecules, 2011, 44:4479 doi: 10.1021/ma200586w
9. [9]
  Huang, N., Guan, Y., Zhu, X.X. and Zhang, Y.J., Chem. Phys. Chem., 2014, 15:1785 doi: 10.1002/cphc.v15.9
10. [10]
  Li, L.H., Zhu, Y., Li, B. and Gao, C.Y., Langmuir, 2008, 24:13632 doi: 10.1021/la802556e
11. [11]
  Li, Y.P., Xiao, W.W., Xiao, K., Berti, L., Luo, J., Tseng, H.P., Fung, G. and Lam, K.S., Angew. Chem. Int. Ed., 2012, 124:2918 doi: 10.1002/ange.201107144
12. [12]
  Meng, F.H., Li, Y.L., Zhu, L., Liu, Z.Z., Cheng, R., Cui, J.H., Ji, S.J. and Zhong, Z.Y., Angew. Chem. Int. Ed., 2009, 48:9914 doi: 10.1002/anie.v48:52
13. [13]
  Zhang, H., Mardyani, S., Chan, W.C.W. and Kumacheva, E., Biomacromolecules, 2006, 7:1568 doi: 10.1021/bm050912z
14. [14]
  Roberts, M.J., Bentley, M.D. and Harris, J.M., Adv. Drug Delivery Rev., 2002, 54:459 doi: 10.1016/S0169-409X(02)00022-4
15. [15]
  Cartei, F., Cartei, G., Ceschia, V., Pacor, S. and Sava, G., Curr. Therap. Res. Clin. Exp., 1991, 50:530
16. [16]
  Tao, L., Mantovani, G., Lecolley, F. and Haddleton, D.M., J. Am. Chem. Soc., 2004, 126:13220 doi: 10.1021/ja0456454
17. [17]
  Bontempo, D. and Maynard, H.D., J. Am. Chem. Soc., 2005, 127:6508 doi: 10.1021/ja042230+
18. [18]
  Boyer, C., Bulmus, V., Liu, J.Q., Davis, T.P., Stenzel, M.H. and Barner-Kowollik, C., J. Am. Chem. Soc., 2007, 129:7145 doi: 10.1021/ja070956a
19. [19]
  Heredia, K.L., Bontempo, D., Ly, T., Byers, J.T., Halstenberg, S. and Maynard, H.D., J. Am. Chem. Soc., 2005, 127:16955 doi: 10.1021/ja054482w
20. [20]
  Tao, L., Liu, J.Q., Xu, J.T. and Davis, T.P., Org. Biomol. Chem., 2009, 7:3481 doi: 10.1039/b907061c
21. [21]
  Kochendoerfer, G.G., Chen, S.Y., Mao, F., Cressman, S., Traviglia, S., Shao, H., Hunter, C.L., Low, D.W., Cagle, E.N., Carnevali, M., Gueriguian, V., Keogh, P.J., Porter, H., Stratton, S.M., Wiedeke, M.C., Wilken, J., Tang, J., Levy, J.J., Miranda, L.P., Crnogorac, M.M., Kalbag, S., Botti, P., Schindler-Horvat, J., Savatski, L., Adamson, J.W., Kung, A., Kent, S.B.H. and Bradburne, J.A., Science, 2003, 299:884 doi: 10.1126/science.1079085
22. [22]
  Lindhoud, S., Voorhaar, L., Vries, R.D., Schweins, R., Stuart, M.A.C. and Norde, W., Langmuir, 2009, 25:11425 doi: 10.1021/la901591p
23. [23]
  Ganguli, S., Yoshimoto, K., Tomita, S., Sakuma, H., Matsuoka, T., Shiraki, K. and Nagasaki, Y., J. Am. Chem. Soc., 2009, 131:6549 doi: 10.1021/ja900786z
24. [24]
  Danial, M., Klok, H., Norde, W. and Stuart, M.A.C., Langmuir, 2007, 23:8003 doi: 10.1021/la700573j
25. [25]
  Gummel, J., Boue, F., Deme, B. and Cousin, F., J. Phys. Chem. B, 2006, 110:24837 doi: 10.1021/jp064383k
26. [26]
  Harada, A. and Kataoka, K., Macromolecules, 1998, 31:288 doi: 10.1021/ma971277v
27. [27]
  Oncel, S., Uzun, L., Garipcan, B. and Denizli, A., Ind. Eng. Chem. Res., 2005, 44:7049 doi: 10.1021/ie0506318
28. [28]
  Beierle, J.M., Yoshimatsu, K., Chou, B., Mathews, M.A.A., Lesel, B.K. and Shea, K.J., Angew. Chem. Int. Ed., 2014, 53:9275 doi: 10.1002/anie.201404881
29. [29]
  Dupin, D., Rosselgong, J., Armes, S.P. and Routh, A., Langmuir, 2007, 23:4035 doi: 10.1021/la063278z
30. [30]
  Yin, J., Dupin, D., Li, J.F., Armes, S.P. and Liu, S.Y., Langmuir, 2008, 24:9334 doi: 10.1021/la8014282
31. [31]
  Tanaka, T. and Fillmore, D.J., J. Chem. Phys., 1979, 70:1214 doi: 10.1063/1.437602
32. [32]
  Subotic, D.V. and Wu, X.Y., Ind. Eng. Chem. Res., 1997, 36:1302 doi: 10.1021/ie9604987
33. [33]
  Zhou, S.Q. and Chu, B., J. Phys. Chem. B, 1998, 102:1364 doi: 10.1021/jp972990p
34. [34]
  Shugar, D., Biochim. Biophys. Acta, 1952, 8:302 doi: 10.1016/0006-3002(52)90045-0
35. [35]
  Yin, J., Shi, S.Y., Hu, J.M. and Liu, S.Y., Langmuir, 2014, 30:9551 doi: 10.1021/la501918s
36. [36]
  Yin, J., Guan, X.F., Wang, D. and Liu, S.Y., Langmuir, 2009, 25:11367 doi: 10.1021/la901377h
37. [37]
  Plamper, F.A., Becker, H., Lanzendorfer, M., Patel, M., Wittemann, A., Ballauf, M. and Muller, A.H.E., Macromol. Chem. Phys., 2005, 206:1813 doi: 10.1002/(ISSN)1521-3935
38. [38]
  Olea, A.F. and Thomas, J.K., Macromolecules, 1989, 22:1165 doi: 10.1021/ma00193a029
39. [39]
  Shibayama, M. and Nagai, K., Macromolecules, 1999, 32:7461 doi: 10.1021/ma990719v
40. [40]
  Nakimbugwe, D., Masschalck, B., Deckers, D., Callewaert, L., Aertsen, A. and Michiels, C.W., FEMS Microbiol. Lett., 2006, 259:41 doi: 10.1111/fml.2006.259.issue-1
1. [1]
  Huan Hu , Ying Zhang , Shi-Shuang Huang , Zhi-Gang Li , Yungui Liu , Rui Feng , Wei Li . Temperature- and pressure-responsive photoluminescence in a 1D hybrid lead halide. Chinese Journal of Structural Chemistry, 2024, 43(10): 100395-100395. doi: 10.1016/j.cjsc.2024.100395
2. [2]
  Qiangwei Wang , Huijiao Liu , Mengjie Wang , Haojie Zhang , Jianda Xie , Xuanwei Hu , Shiming Zhou , Weitai Wu . Observation of high ionic conductivity of polyelectrolyte microgels in salt-free solutions. Chinese Chemical Letters, 2024, 35(4): 108743-. doi: 10.1016/j.cclet.2023.108743
3. [3]
  Zixuan Zhu , Xianjin Shi , Yongfang Rao , Yu Huang . Recent progress of MgO-based materials in CO₂ adsorption and conversion: Modification methods, reaction condition, and CO₂ hydrogenation. Chinese Chemical Letters, 2024, 35(5): 108954-. doi: 10.1016/j.cclet.2023.108954
4. [4]
  Yue Li , Minghao Fan , Conghui Wang , Yanxun Li , Xiang Yu , Jun Ding , Lei Yan , Lele Qiu , Yongcai Zhang , Longlu Wang . 3D layer-by-layer amorphous MoS_x assembled from [Mo₃S₁₃]^2- clusters for efficient removal of tetracycline: Synergy of adsorption and photo-assisted PMS activation. Chinese Chemical Letters, 2024, 35(9): 109764-. doi: 10.1016/j.cclet.2024.109764
5. [5]
  Fengyun Li , Zerong Pei , Shuting Chen , Gen li , Mengyang Liu , Liqin Ding , Jingbo Liu , Feng Qiu . Multifunctional nano-herb based on tumor microenvironment for enhanced tumor therapy of gambogic acid. Chinese Chemical Letters, 2024, 35(5): 108752-. doi: 10.1016/j.cclet.2023.108752
6. [6]
  Fangzhou Wang , Wentong Gao , Chenghui Li . A weak but inert hindered urethane bond for high-performance dynamic polyurethane polymers. Chinese Chemical Letters, 2024, 35(5): 109305-. doi: 10.1016/j.cclet.2023.109305
7. [7]
  Dan-Ying Xing , Xiao-Dan Zhao , Chuan-Shu He , Bo Lai . Kinetic study and DFT calculation on the tetracycline abatement by peracetic acid. Chinese Chemical Letters, 2024, 35(9): 109436-. doi: 10.1016/j.cclet.2023.109436
8. [8]
  Linshan Peng , Qihang Peng , Tianxiang Jin , Zhirong Liu , Yong Qian . Highly efficient capture of thorium ion by citric acid-modified chitosan gels from aqueous solution. Chinese Chemical Letters, 2024, 35(5): 108891-. doi: 10.1016/j.cclet.2023.108891
9. [9]
  Xiao-Hong Yi , Chong-Chen Wang . Metal-organic frameworks on 3D interconnected macroporous sponge foams for large-scale water decontamination: A mini review. Chinese Chemical Letters, 2024, 35(5): 109094-. doi: 10.1016/j.cclet.2023.109094
10. [10]
  Haodong Wang , Xiaoxu Lai , Chi Chen , Pei Shi , Houzhao Wan , Hao Wang , Xingguang Chen , Dan Sun . Novel 2D bifunctional layered rare-earth hydroxides@GO catalyst as a functional interlayer for improved liquid-solid conversion of polysulfides in lithium-sulfur batteries. Chinese Chemical Letters, 2024, 35(5): 108473-. doi: 10.1016/j.cclet.2023.108473
11. [11]
  Dan Luo , Jinya Tian , Jianqiao Zhou , Xiaodong Chi . Anthracene-bridged "Texas-sized" box for the simultaneous detection and uptake of tryptophan. Chinese Chemical Letters, 2024, 35(9): 109444-. doi: 10.1016/j.cclet.2023.109444
12. [12]
  Heng Zhang . Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism. University Chemistry, 2024, 39(4): 395-400. doi: 10.3866/PKU.DXHX202310047
13. [13]
  Jing Wang , Pingping Li , Yuehui Wang , Yifan Xiu , Bingqian Zhang , Shuwen Wang , Hongtao Gao . Treatment and Discharge Evaluation of Phosphorus-Containing Wastewater. University Chemistry, 2024, 39(5): 52-62. doi: 10.3866/PKU.DXHX202309097
14. [14]
  Guang Huang , Lei Li , Dingyi Zhang , Xingze Wang , Yugai Huang , Wenhui Liang , Zhifen Guo , Wenmei Jiao . Cobalt’s Valor, Nickel’s Foe: A Comprehensive Chemical Experiment Utilizing a Cobalt-based Imidazolate Framework for Nickel Ion Removal. University Chemistry, 2024, 39(8): 174-183. doi: 10.3866/PKU.DXHX202311051
15. [15]
  Yi Zhang , Biao Wang , Chao Hu , Muhammad Humayun , Yaping Huang , Yulin Cao , Mosaad Negem , Yigang Ding , Chundong Wang . Fe–Ni–F electrocatalyst for enhancing reaction kinetics of water oxidation. Chinese Journal of Structural Chemistry, 2024, 43(2): 100243-100243. doi: 10.1016/j.cjsc.2024.100243
16. [16]
  Yi Herng Chan , Zhe Phak Chan , Serene Sow Mun Lock , Chung Loong Yiin , Shin Ying Foong , Mee Kee Wong , Muhammad Anwar Ishak , Ven Chian Quek , Shengbo Ge , Su Shiung Lam . Thermal pyrolysis conversion of methane to hydrogen (H₂): A review on process parameters, reaction kinetics and techno-economic analysis. Chinese Chemical Letters, 2024, 35(8): 109329-. doi: 10.1016/j.cclet.2023.109329
17. [17]
  Cunjun Li , Wencong Liu , Xianlei Chen , Liang Li , Shenyu Lan , Mingshan Zhu . Adsorption and activation of peroxymonosulfate on BiOCl for carbamazepine degradation: The role of piezoelectric effect. Chinese Chemical Letters, 2024, 35(10): 109652-. doi: 10.1016/j.cclet.2024.109652
18. [18]
  Shuqi Yu , Yu Yang , Keisuke Kuroda , Jian Pu , Rui Guo , Li-An Hou . Selective removal of Cr(Ⅵ) using polyvinylpyrrolidone and polyacrylamide co-modified MoS₂ composites by adsorption combined with reduction. Chinese Chemical Letters, 2024, 35(6): 109130-. doi: 10.1016/j.cclet.2023.109130
19. [19]
  Shuanglin TIAN , Tinghong GAO , Yutao LIU , Qian CHEN , Quan XIE , Qingquan XIAO , Yongchao LIANG . First-principles study of adsorption of Cl₂ and CO gas molecules by transition metal-doped g-GaN. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1189-1200. doi: 10.11862/CJIC.20230482
20. [20]
  Xiao Li , Wanqiang Yu , Yujie Wang , Ruiying Liu , Qingquan Yu , Riming Hu , Xuchuan Jiang , Qingsheng Gao , Hong Liu , Jiayuan Yu , Weijia Zhou . Metal-encapsulated nitrogen-doped carbon nanotube arrays electrode for enhancing sulfion oxidation reaction and hydrogen evolution reaction by regulating of intermediate adsorption. Chinese Chemical Letters, 2024, 35(8): 109166-. doi: 10.1016/j.cclet.2023.109166

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(638)
HTML views(1)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142