Citation: Ji-jie Wen, Hong-guang Lu, De-e Liu, Hui Gao. Fabrication of Macroporous Protein-containing Films through the Reverse Emulsions Approach Featuring β-Cyclodextrin-conjugated PEG-PLGA Copolymers[J]. Chinese Journal of Polymer Science, ;2016, 34(6): 730-738. doi: 10.1007/s10118-016-1789-1 shu

Fabrication of Macroporous Protein-containing Films through the Reverse Emulsions Approach Featuring β-Cyclodextrin-conjugated PEG-PLGA Copolymers

School of Chemistry and Chemical Engineering, Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology, Tianjin 300384, China

Corresponding author: Hong-guang Lu, hglu@tjut.edu.cn Hui Gao, hgao@tjut.edu.cn;ghhigher@hotmail.com
Received Date: 11 December 2015
Revised Date: 31 January 2016
Accepted Date: 22 December 2015

Fund Project: Program for New Century Excellent Talents in Universities No. NCET-11-1063the National Natural Science Foundation of China Nos. 21374079 and 21244004

A series of β-cyclodextrin-conjugated 4-arm poly(ethylene glycol)-poly(lactide-co-glycolide) (4-arm PEG-PLGA) copolymers were synthesized by a ring-opening polymerization of D,L-lactide and glycolide using 4-arm PEG as initiator, and then conjugated with mono(6-ethylenediamine-6-deoxy)-β-cyclodextrin (CDen) or ethylenediamino-bridged bis-β-CD (BCDen). The chemical structures of copolymers were confirmed by ¹H-NMR and FTIR spectroscopy. The β-CD-conjugated PEG-PLGA formed stable reverse micelles due to the formation of β-CD and bovine serum albumin (BSA) inclusion complexation, which could accommodate BSA in the organic solvent with improved encapsulation efficiency. Moreover, we demonstrated a one-step approach to construct macroporous protein-containing films using these reverse micelles. The films with ordered pore arrays were directly prepared from reverse micelles. Interestingly, the protein was totally located in the whole matrix except for the pores.
- β-Cyclodextrin,
- Copolymer,
- Reverse micelles,
- Ordered porous structures,
- Proteins
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