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2021 Volume 49 Issue 7
2021, 49(7): 1063-1075
doi: 10.19756/j.issn.0253-3820.211140
Abstract:
As a significant spectral analysis tool, surface-enhanced Raman scattering (SERS) technique demonstrates several distinctive attributes like in situ nondestructive nature, finger recognition and single-molecule level detection, which has a great potential in the fields of food safety, environmental monitoring, and biological analysis, etc. Herein, this review focuses on the current progress of SERS-based biosensing and bioimaging, and further discusses the possible development tendencies and prospects of SERS on bioanalysis in coming future.
As a significant spectral analysis tool, surface-enhanced Raman scattering (SERS) technique demonstrates several distinctive attributes like in situ nondestructive nature, finger recognition and single-molecule level detection, which has a great potential in the fields of food safety, environmental monitoring, and biological analysis, etc. Herein, this review focuses on the current progress of SERS-based biosensing and bioimaging, and further discusses the possible development tendencies and prospects of SERS on bioanalysis in coming future.
2021, 49(7): 1076-1088
doi: 10.19756/j.issn.0253-3820.201554
Abstract:
As a kind of carbon-based luminescent nanomaterials, carbon quantum dots (CQDs) have been widely used in the fields of biosensor and biological imaging because of their low toxicity, adjustable optical properties, low cost, excellent light stability and good biocompatibility. Although there are various methods to synthesize CQDs, the green synthesis method using biomass-based natural raw materials can convert low-value wastes into high-value biomass-based CQDs, which is the trend to realize the energy sustainable development in the future. In this review, we summarized the synthesis methods of biomass-based CQDs and their latest development in the field of sensing and imaging. Meanwhile, the application prospect and development direction of biomass-based CQDs in the field of sensing and imaging were also prospected.
As a kind of carbon-based luminescent nanomaterials, carbon quantum dots (CQDs) have been widely used in the fields of biosensor and biological imaging because of their low toxicity, adjustable optical properties, low cost, excellent light stability and good biocompatibility. Although there are various methods to synthesize CQDs, the green synthesis method using biomass-based natural raw materials can convert low-value wastes into high-value biomass-based CQDs, which is the trend to realize the energy sustainable development in the future. In this review, we summarized the synthesis methods of biomass-based CQDs and their latest development in the field of sensing and imaging. Meanwhile, the application prospect and development direction of biomass-based CQDs in the field of sensing and imaging were also prospected.
2021, 49(7): 1089-1105
doi: 10.19756/j.issn.0253-3820.211094
Abstract:
Supraparticles (SPs) are the nano-agglomerates with a certain shape and hierarchical structure, which are self-assembled by same or different kinds of individual inorganic nanocrystals. SPs not only exhibit collective properties and/or synergistic effects, but their diverse shapes and devisable spatial structures provide multi-scale and multi-dimensional possibilities for the interactions with various biological systems. Thus, SPs show a variety of application potentials in bio-sensing, bio-imaging, diagnosis and even therapy, etc. In this review, the progress of SPs fabrication, properties, and their applications in bioimaging field in recent years are summarized, and the main problems and future development of their fabrication and applications in bio-medicine are discussed.
Supraparticles (SPs) are the nano-agglomerates with a certain shape and hierarchical structure, which are self-assembled by same or different kinds of individual inorganic nanocrystals. SPs not only exhibit collective properties and/or synergistic effects, but their diverse shapes and devisable spatial structures provide multi-scale and multi-dimensional possibilities for the interactions with various biological systems. Thus, SPs show a variety of application potentials in bio-sensing, bio-imaging, diagnosis and even therapy, etc. In this review, the progress of SPs fabrication, properties, and their applications in bioimaging field in recent years are summarized, and the main problems and future development of their fabrication and applications in bio-medicine are discussed.
2021, 49(7): 1106-1120
doi: 10.19756/j.issn.0253-3820.211090
Abstract:
Upconversion nanoparticles can emit high-energy photons in visible region under the excitation of near-infrared light. Compared with traditional fluorescent materials, upconversion nanoparticles show better tissue penetration depth and biocompatibility, and also reduce the fluorescence interference of biological cells and tissues. Therefore, upconversion nanoparticles have broad application prospects in the field of highly sensitivie biological imaging. Erbium (Er) has a strong absorption in the near infrared Ⅱ region (1500 nm), and can emit red or green light. With the excellent optical properties, Erbium is usually doped in the upconversion nanoparticles as the emission center. However, due to the problems of surface quenching and energy countercurrent, the upconversion nanoparticles doped with Er3+ have limited luminescence efficiency and low biological imaging performance. In this review, the development of Er3+ doped upconversion nanoparticles in optical properties optimization and biological imaging applications are summarized, and the latest progress and development prospects of Er3+ doped upconversion nanoparticle materials in the domain of temporal pathway imaging are discussed and prospected.
Upconversion nanoparticles can emit high-energy photons in visible region under the excitation of near-infrared light. Compared with traditional fluorescent materials, upconversion nanoparticles show better tissue penetration depth and biocompatibility, and also reduce the fluorescence interference of biological cells and tissues. Therefore, upconversion nanoparticles have broad application prospects in the field of highly sensitivie biological imaging. Erbium (Er) has a strong absorption in the near infrared Ⅱ region (1500 nm), and can emit red or green light. With the excellent optical properties, Erbium is usually doped in the upconversion nanoparticles as the emission center. However, due to the problems of surface quenching and energy countercurrent, the upconversion nanoparticles doped with Er3+ have limited luminescence efficiency and low biological imaging performance. In this review, the development of Er3+ doped upconversion nanoparticles in optical properties optimization and biological imaging applications are summarized, and the latest progress and development prospects of Er3+ doped upconversion nanoparticle materials in the domain of temporal pathway imaging are discussed and prospected.
2021, 49(7): 1121-1132
doi: 10.19756/j.issn.0253-3820.201734
Abstract:
Chemodynamic therapy (CDT) is receiving increasing attention for tumor therapy. In the presence of catalysts, H2O2 can be converted into ·OH that possesses stronger oxidation and higher toxicity through the Fenton or Fenton-like reaction utilizing the unique feature of tumor microenvironments such as weak acidity and excessive H2O2. The generated ·OH can disrupt the ROS homeostasis in tumor cells and leads to severe oxidative stress, which can cause DNA necrosis, protein inactivation, lipid oxidation and finally induce cell apoptosis or necrosis. CDT is characterized by high specificity and independence and it is especially suitable for the treatment of tumors in deep tissues. However, the development of CDT is still in its infancy, and its therapeutic efficiency is still not satisfactory. To improve the efficacy of CDT, three different strategies have been introduced, i.e., changing tumor microenvironment, selecting appropriate catalyst and substrate, and combining with other treatment methods. In this paper, we summarize the recent development of CDT in tumor treatment and briefly point out its application prospects.
Chemodynamic therapy (CDT) is receiving increasing attention for tumor therapy. In the presence of catalysts, H2O2 can be converted into ·OH that possesses stronger oxidation and higher toxicity through the Fenton or Fenton-like reaction utilizing the unique feature of tumor microenvironments such as weak acidity and excessive H2O2. The generated ·OH can disrupt the ROS homeostasis in tumor cells and leads to severe oxidative stress, which can cause DNA necrosis, protein inactivation, lipid oxidation and finally induce cell apoptosis or necrosis. CDT is characterized by high specificity and independence and it is especially suitable for the treatment of tumors in deep tissues. However, the development of CDT is still in its infancy, and its therapeutic efficiency is still not satisfactory. To improve the efficacy of CDT, three different strategies have been introduced, i.e., changing tumor microenvironment, selecting appropriate catalyst and substrate, and combining with other treatment methods. In this paper, we summarize the recent development of CDT in tumor treatment and briefly point out its application prospects.
2021, 49(7): 1133-1141
doi: 10.19756/j.issn.0253-3820.211086
Abstract:
Under the trigger of endogenous stimuli (such as pH, overexpressed enzymes, redox, etc.) or exogenous stimuli (such as light, temperature, magnetic fields, etc.), stimuli-responsive nanomaterials can change their structures and properties in tumor tissues, so as to achieve precise drug delivery and imaging. Due to the differences in structure, composition and function, these stimuli-responsive nanomaterials have exhibited diverse applications, such as photothermal therapy, chemotherapy or imaging by triggered drug delivery. To explore the potential applications of stimuli-responsive nanomaterials, this review focuses on exogenous light-responsive and/or endogenous enzyme-responsive nanomaterials and summarizes the research progress in the areas of tumor-related therapy and bioimaging in the past few years.
Under the trigger of endogenous stimuli (such as pH, overexpressed enzymes, redox, etc.) or exogenous stimuli (such as light, temperature, magnetic fields, etc.), stimuli-responsive nanomaterials can change their structures and properties in tumor tissues, so as to achieve precise drug delivery and imaging. Due to the differences in structure, composition and function, these stimuli-responsive nanomaterials have exhibited diverse applications, such as photothermal therapy, chemotherapy or imaging by triggered drug delivery. To explore the potential applications of stimuli-responsive nanomaterials, this review focuses on exogenous light-responsive and/or endogenous enzyme-responsive nanomaterials and summarizes the research progress in the areas of tumor-related therapy and bioimaging in the past few years.
2021, 49(7): 1142-1153
doi: 10.19756/j.issn.0253-3820.201702
Abstract:
With the development of nanotechnology, developing novel nanoplatforms simultaneously integrating bioimaging and treatment for precise cancer theranostics has become a research hotspot. As one of the emerging therapies, photodynamic therapy (PDT) has many advantages such as high specificity, controllability and low side effects, which has attracted extensive research attention. Recently, PDT based on nanomaterials has overcome the limitations of traditional PDT such as low stability, low tumor-targeting ability and phototoxicity, and has shown great application potential. Furthermore, the technologies of bioimaging and PDT can be integrated into a single nanoplatform to realize cancer theranostics. So far, a variety of imaging modes have been applied to the research of PDT based on nanomaterials, such as fluorescence imaging, magnetic resonance imaging (MRI), computer tomography (CT) imaging, ultrasound (US) imaging, photoacoustic (PA) imaging, positron emission tomography (PET) imaging, and single-photon emission computed tomography (SPECT) imaging. This article summarizes the use of multifunctional nanomaterials for bioimaging-guided PDT. Finally, the current challenges and prospects for future development are discussed.
With the development of nanotechnology, developing novel nanoplatforms simultaneously integrating bioimaging and treatment for precise cancer theranostics has become a research hotspot. As one of the emerging therapies, photodynamic therapy (PDT) has many advantages such as high specificity, controllability and low side effects, which has attracted extensive research attention. Recently, PDT based on nanomaterials has overcome the limitations of traditional PDT such as low stability, low tumor-targeting ability and phototoxicity, and has shown great application potential. Furthermore, the technologies of bioimaging and PDT can be integrated into a single nanoplatform to realize cancer theranostics. So far, a variety of imaging modes have been applied to the research of PDT based on nanomaterials, such as fluorescence imaging, magnetic resonance imaging (MRI), computer tomography (CT) imaging, ultrasound (US) imaging, photoacoustic (PA) imaging, positron emission tomography (PET) imaging, and single-photon emission computed tomography (SPECT) imaging. This article summarizes the use of multifunctional nanomaterials for bioimaging-guided PDT. Finally, the current challenges and prospects for future development are discussed.
2021, 49(7): 1154-1165
doi: 10.19756/j.issn.0253-3820.211087
Abstract:
Single molecule array technology (Simoa) is a digital enzyme-linked immunosorbent assay (ELISA) that performs single-molecule enzymatic reactions in microwell with a volume of femtoliters and enables fluorescence imaging. Simoa has many advantages such as rapidity, high sensitivity and wide detection range, which has shown great application potential in the fields of bioanalysis, disease diagnosis and prognosis evaluation. On the basis of summarizing the principles and key technologies of Simoa, in this article, the application progress of Simoa in the fields of neurodegenerative diseases, tumors, infectious diseases, etc., were mainly reviewed, and application prospects of Simoa were anticipated.
Single molecule array technology (Simoa) is a digital enzyme-linked immunosorbent assay (ELISA) that performs single-molecule enzymatic reactions in microwell with a volume of femtoliters and enables fluorescence imaging. Simoa has many advantages such as rapidity, high sensitivity and wide detection range, which has shown great application potential in the fields of bioanalysis, disease diagnosis and prognosis evaluation. On the basis of summarizing the principles and key technologies of Simoa, in this article, the application progress of Simoa in the fields of neurodegenerative diseases, tumors, infectious diseases, etc., were mainly reviewed, and application prospects of Simoa were anticipated.
2021, 49(7): 1166-1175
doi: 10.19756/j.issn.0253-3820.211041
Abstract:
Nanopores technique is a label-free, high-throughput, high-spatial-temporal resolution electrochemical measurement method for single-molecule level analysis, which is widely used for single-entity measurements such as single molecules, single particles, and single cells. Nanopores not only have adjustable three-dimensional space to confine single analytes, but also possess an ability to enhance the electrical and magnetic fields. In short, the nanopores exhibit unique sub-wavelength optical properties. In this review, we introduce the principle of nanopore electrochemical analysis, and give a perspective and detailed overview of its application in electro-optical binding measurements.
Nanopores technique is a label-free, high-throughput, high-spatial-temporal resolution electrochemical measurement method for single-molecule level analysis, which is widely used for single-entity measurements such as single molecules, single particles, and single cells. Nanopores not only have adjustable three-dimensional space to confine single analytes, but also possess an ability to enhance the electrical and magnetic fields. In short, the nanopores exhibit unique sub-wavelength optical properties. In this review, we introduce the principle of nanopore electrochemical analysis, and give a perspective and detailed overview of its application in electro-optical binding measurements.
2021, 49(7): 1176-1187
doi: 10.19756/j.issn.0253-3820.211073
Abstract:
Diversified composition and tailorable properties of nanomaterials have provided infinite possibilities for the development of mass spectrometric methods. With the blooming innovation of mass spectrometry technology in recent years, researchers have begun to expand the application of nanomaterials-assisted mass spectrometry from mere detection to imaging. By using nanomaterials as matrices for assisting desorption/ionization or carriers of signal molecules, mass spectrometry imaging can not only provide molecular information of unknown compounds, but also achieve accurate profiling of spatial distribution of biomolecules, drugs, environmental pollutants and other target molecules in tissues and even single cells, thus providing a more intuitive means for physiology and pathology study at tissue or cell level. In this review, the main principles and research progress of nanomaterials facilitated mass spectrometry imaging are summarized, and its future development and potential applications are also prospected.
Diversified composition and tailorable properties of nanomaterials have provided infinite possibilities for the development of mass spectrometric methods. With the blooming innovation of mass spectrometry technology in recent years, researchers have begun to expand the application of nanomaterials-assisted mass spectrometry from mere detection to imaging. By using nanomaterials as matrices for assisting desorption/ionization or carriers of signal molecules, mass spectrometry imaging can not only provide molecular information of unknown compounds, but also achieve accurate profiling of spatial distribution of biomolecules, drugs, environmental pollutants and other target molecules in tissues and even single cells, thus providing a more intuitive means for physiology and pathology study at tissue or cell level. In this review, the main principles and research progress of nanomaterials facilitated mass spectrometry imaging are summarized, and its future development and potential applications are also prospected.
2021, 49(7): 1188-1197
doi: 10.19756/j.issn.0253-3820.211123
Abstract:
Electrochemiluminescence (ECL) is a luminous phenomenon in which the excited state luminophore is generated by dark electrochemical reactions in solutions. Due to its near-zero background, high sensitivity, good spatiotemporal controllability, fast detection and wide dynamic range, ECL has manifested itself to be one of the most successful techniques in in vitro diagnosis and clinical detection. In addition, ECL imaging possesses the unique advantages such as high-throughput and visualization. As a powerful surface analysis technology, ECL imaging has been successfully employed in material surface/ interface analysis and bioanalysis. Given that the ECL intensity is highly dependent on the properties of electrode surface, ECL imaging can be used to investigate electron-transfer properties and the distribution of electrochemical activity of chemically-modified electrode. In the first part of this review, we briefly introduce the background of ECL technology and describe the generation mechanisms of ECL systems. Then we focus on the recent research progress of imaging analysis based on ECL microscopy, including mechanism rationalization, latent fingerprint visualization and single cell analysis. Finally, some perspectives and future directions of ECL are presented.
Electrochemiluminescence (ECL) is a luminous phenomenon in which the excited state luminophore is generated by dark electrochemical reactions in solutions. Due to its near-zero background, high sensitivity, good spatiotemporal controllability, fast detection and wide dynamic range, ECL has manifested itself to be one of the most successful techniques in in vitro diagnosis and clinical detection. In addition, ECL imaging possesses the unique advantages such as high-throughput and visualization. As a powerful surface analysis technology, ECL imaging has been successfully employed in material surface/ interface analysis and bioanalysis. Given that the ECL intensity is highly dependent on the properties of electrode surface, ECL imaging can be used to investigate electron-transfer properties and the distribution of electrochemical activity of chemically-modified electrode. In the first part of this review, we briefly introduce the background of ECL technology and describe the generation mechanisms of ECL systems. Then we focus on the recent research progress of imaging analysis based on ECL microscopy, including mechanism rationalization, latent fingerprint visualization and single cell analysis. Finally, some perspectives and future directions of ECL are presented.
2021, 49(7): 1198-1207
doi: 10.19756/j.issn.0253-3820.210405
Abstract:
DNA driven inorganic nanostructures not only display great flexibility in structure regulation and simplicity in surface modification but also exhibit specific optical properties, which shows numerous advantages in bio-sensing, bio-imaging, in situ analysis of living cells. Scientists developed a series of detection strategies for in situ analysis of important targets in living cells, which can be used for early diagnosis and treatment of serious diseases (such as cancers) and spurred the development of living systems. In this review, we introduce the biological applications of DNA driven inorganic nanostructures, which is anticipated to guide the development of living system, medical field and biological area further.
DNA driven inorganic nanostructures not only display great flexibility in structure regulation and simplicity in surface modification but also exhibit specific optical properties, which shows numerous advantages in bio-sensing, bio-imaging, in situ analysis of living cells. Scientists developed a series of detection strategies for in situ analysis of important targets in living cells, which can be used for early diagnosis and treatment of serious diseases (such as cancers) and spurred the development of living systems. In this review, we introduce the biological applications of DNA driven inorganic nanostructures, which is anticipated to guide the development of living system, medical field and biological area further.
2021, 49(7): 1208-1217
doi: 10.19756/j.issn.0253-3820.201701
Abstract:
The lysosome-targeting carbon dots with excellent water solubility and strong blue emission were synthesized through a one-step functional modification for long-term lysosome imaging in living cells. Based on the carboxyl rich carbon dots synthesized by oxidizing carbon black in refluxing HNO3, N,N-dimethylethylenediamine (DMEDA) was chemically linked onto the carboxyl rich carbon dots through acylation reaction to obtain DMEDA modified carbon dots (M-CDs). The infrared spectrum of M-CDs showed the characteristic peak of acylamide (O=C-NH-) at 1658 cm–1, indicating the successful modification of DMEDA. The quantum yield of M-CDs was 15.6%, which was about 27 times higher than the original carboxyl rich carbon dots, meeting the requirement of cell imaging. The probe entered cells through temperature-dependent endocytosis way and the co-localization experiment with commercial dye Lyso-Tracker red showed that the probe localized in lysosomes and could be used as a universal lysosome tracker in different cell lines. Moreover, compared to commercially available Lyso-Tracker blue, M-CDs were more photostable under UV light. The fluorescence of M-CDs was still brightness after time-lapsed imaging by confocal microscopy for 120 min with an interval of 5 min in living cells, implying that the M-CDs could be used for long-term imaging of lysosomes.
The lysosome-targeting carbon dots with excellent water solubility and strong blue emission were synthesized through a one-step functional modification for long-term lysosome imaging in living cells. Based on the carboxyl rich carbon dots synthesized by oxidizing carbon black in refluxing HNO3, N,N-dimethylethylenediamine (DMEDA) was chemically linked onto the carboxyl rich carbon dots through acylation reaction to obtain DMEDA modified carbon dots (M-CDs). The infrared spectrum of M-CDs showed the characteristic peak of acylamide (O=C-NH-) at 1658 cm–1, indicating the successful modification of DMEDA. The quantum yield of M-CDs was 15.6%, which was about 27 times higher than the original carboxyl rich carbon dots, meeting the requirement of cell imaging. The probe entered cells through temperature-dependent endocytosis way and the co-localization experiment with commercial dye Lyso-Tracker red showed that the probe localized in lysosomes and could be used as a universal lysosome tracker in different cell lines. Moreover, compared to commercially available Lyso-Tracker blue, M-CDs were more photostable under UV light. The fluorescence of M-CDs was still brightness after time-lapsed imaging by confocal microscopy for 120 min with an interval of 5 min in living cells, implying that the M-CDs could be used for long-term imaging of lysosomes.
2021, 49(7): 1218-1227
doi: 10.19756/j.issn.0253-3820.211066
Abstract:
The TiO2 nanorods (TiO2 NRs) were prepared by hydrothermal method, and a TiO2 NRs non-metallic surface-enhanced Raman spectroscopy (SERS) biosensor was constructed. With copper phthalocyanine (CuPc) as the adsorbed molecule, the developed TiO2 NRs SERS biosensor revealed remarkable Raman activity. Through experimental data and theoretical calculations, it was found that significant SERS enhancement (Enhancement factor (EF) =3.18×108) of CuPc was due to the chemical mechanism (CM) based on charge transfer. By utilizing the significant Raman response of CuPc on the TiO2 NRs and the specific recognition of telomere G-quadruplex, TiO2 NRs was used as a SERS biosensor for quantitative and sensitive detection of telomerase activity, with a detection limit down to 2.85×10-16 IU/L. In addition, due to the high selectivity and high sensitivity, the SERS biosensor was used to determine the telomerase activity as well as the cell numbers in Hela cells, making it an effective way to detect telomerase activity in other cells. This work not only established an approach for studying the Raman enhancement mechanism of semiconductor based on CM, but also paved a new way for the detection of related substances in clinical diagnosis and cell biomedical analysis.
The TiO2 nanorods (TiO2 NRs) were prepared by hydrothermal method, and a TiO2 NRs non-metallic surface-enhanced Raman spectroscopy (SERS) biosensor was constructed. With copper phthalocyanine (CuPc) as the adsorbed molecule, the developed TiO2 NRs SERS biosensor revealed remarkable Raman activity. Through experimental data and theoretical calculations, it was found that significant SERS enhancement (Enhancement factor (EF) =3.18×108) of CuPc was due to the chemical mechanism (CM) based on charge transfer. By utilizing the significant Raman response of CuPc on the TiO2 NRs and the specific recognition of telomere G-quadruplex, TiO2 NRs was used as a SERS biosensor for quantitative and sensitive detection of telomerase activity, with a detection limit down to 2.85×10-16 IU/L. In addition, due to the high selectivity and high sensitivity, the SERS biosensor was used to determine the telomerase activity as well as the cell numbers in Hela cells, making it an effective way to detect telomerase activity in other cells. This work not only established an approach for studying the Raman enhancement mechanism of semiconductor based on CM, but also paved a new way for the detection of related substances in clinical diagnosis and cell biomedical analysis.
2021, 49(7): 1228-1236
doi: 10.19756/j.issn.0253-3820.201570
Abstract:
Developing multifunctional nanomaterials is a hot topic for the combination therapy of tumor cells. In this work, a distinctive multifunctional composite (FA-Cu2-xSySe1-y@pDA-BTZ) was fabricated by assembling folic acid (FA) and the anticancer drug bortezomib (BTZ) to the surface of the nonstoichiometric copper chalcogenide nanoparticle (Cu2-xSySe1-y NPs) with typical localized surface plasmon resonance (LSPR) in the near-infrared region coated by polydopamine (Cu2-xSySe1-y@pDA). The FA-Cu2-xSySe1-y@pDA-BTZ showed a good light harvest in the second near-infrared window for bio-application and the photothermal conversion efficiency could reach 30.8%. FA was attached to the surface of the Cu2-xSySe1-y@pDA, so that the nanocomplex could be specifically delivered into cancer cells with overexpressed folate receptor. Meanwhile, BTZ could easily bind with Cu2-xSySe1-y@pDA through the formation of pH-responsive boronate ester bond between boronic acid and catechol groups in polydopamine under weakly alkaline conditions. Under 1064 nm laser irradiation in weakly acidic phosphate buffer, the controlled release of drugs was obtained. Under the laser irradiation (1064 nm), the relative survival rate of the cells was lower than 10%, which could realize the synergistic treatment of photothermal therapy and chemotherapy for tumor cells. The FA-Cu2-xSySe1-y@pDA-BTZ could be used as a new probe in the dark-field imaging for monitoring the therapy. The above results showed that the synthesized FA-Cu2-xSySe1-y@pDA-BTZ multifunctional composite had a good application prospect in the diagnosis and treatment of cancer.
Developing multifunctional nanomaterials is a hot topic for the combination therapy of tumor cells. In this work, a distinctive multifunctional composite (FA-Cu2-xSySe1-y@pDA-BTZ) was fabricated by assembling folic acid (FA) and the anticancer drug bortezomib (BTZ) to the surface of the nonstoichiometric copper chalcogenide nanoparticle (Cu2-xSySe1-y NPs) with typical localized surface plasmon resonance (LSPR) in the near-infrared region coated by polydopamine (Cu2-xSySe1-y@pDA). The FA-Cu2-xSySe1-y@pDA-BTZ showed a good light harvest in the second near-infrared window for bio-application and the photothermal conversion efficiency could reach 30.8%. FA was attached to the surface of the Cu2-xSySe1-y@pDA, so that the nanocomplex could be specifically delivered into cancer cells with overexpressed folate receptor. Meanwhile, BTZ could easily bind with Cu2-xSySe1-y@pDA through the formation of pH-responsive boronate ester bond between boronic acid and catechol groups in polydopamine under weakly alkaline conditions. Under 1064 nm laser irradiation in weakly acidic phosphate buffer, the controlled release of drugs was obtained. Under the laser irradiation (1064 nm), the relative survival rate of the cells was lower than 10%, which could realize the synergistic treatment of photothermal therapy and chemotherapy for tumor cells. The FA-Cu2-xSySe1-y@pDA-BTZ could be used as a new probe in the dark-field imaging for monitoring the therapy. The above results showed that the synthesized FA-Cu2-xSySe1-y@pDA-BTZ multifunctional composite had a good application prospect in the diagnosis and treatment of cancer.
2021, 49(7): 1237-1244
doi: 10.19756/j.issn.0253-3820.201802
Abstract:
Hypoxia is an important feature of solid tumors microenvironment, which is closely related to tumor highly aggressive, metastatic characteristics and poor prognosis. Therefore, it is of great significance to establish an efficient, rapid, sensitive and non-invasive method for detection of cell endogenous hypoxia and exogenous hypoxia. In this study, a ratio fluorescence probe was designed to detect endogenous and exogenous hypoxia levels, and the ratio fluorescence imaging was used to detect intracellular hypoxia. The oxygen sensitive fluorescent dye tris(4,7-biphenyl-1,10)-phenanthroline) ruthenium dichloride ([(Ru(dpp)3)]Cl2) was loaded on mesoporous yolk-shell organosilicon nanoparticle (MYSN) doped with fluorescein isothiocyanate (FITC). The linear response range of the probe was 5-290 μmol/L, the response time was 1 min, and the detection limit was as low as 4.68 μmol/L. Cell experiments showed that the as-prepared probe could be significantly taken up, exhibiting high specificity and high stability in fluorescence imaging of endogenous and exogenous hypoxia.
Hypoxia is an important feature of solid tumors microenvironment, which is closely related to tumor highly aggressive, metastatic characteristics and poor prognosis. Therefore, it is of great significance to establish an efficient, rapid, sensitive and non-invasive method for detection of cell endogenous hypoxia and exogenous hypoxia. In this study, a ratio fluorescence probe was designed to detect endogenous and exogenous hypoxia levels, and the ratio fluorescence imaging was used to detect intracellular hypoxia. The oxygen sensitive fluorescent dye tris(4,7-biphenyl-1,10)-phenanthroline) ruthenium dichloride ([(Ru(dpp)3)]Cl2) was loaded on mesoporous yolk-shell organosilicon nanoparticle (MYSN) doped with fluorescein isothiocyanate (FITC). The linear response range of the probe was 5-290 μmol/L, the response time was 1 min, and the detection limit was as low as 4.68 μmol/L. Cell experiments showed that the as-prepared probe could be significantly taken up, exhibiting high specificity and high stability in fluorescence imaging of endogenous and exogenous hypoxia.
2021, 49(7): 1245-1245
doi: 10.1016/S1872-2040(21)60109-3
Abstract:
2021, 49(7): 1245-1245
doi: 10.1016/S1872-2040(21)60110-X
Abstract:
