2020 Volume 48 Issue 10

2020, 48(10):
[Abstract](622) [FullText HTML] [PDF 6136KB](5)
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Research Progress of Nucleic Acid Detection Technology Platforms for New Coronavirus SARS-CoV-2
SHENG Nan , MA Xue-Ping , PANG Shu-Yun , SONG Qin-Xin , ZOU Bing-Jie , ZHOU Guo-Hua
2020, 48(10): 1279-1287  doi: 10.19756/j.issn.0253-3820.201259
[Abstract](987) [FullText HTML] [PDF 3137KB](17)
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The new coronavirus SARS-CoV-2 has spread to the whole world, seriously threatening human life and disrupting people's lives. SARS-CoV-2 is a highly infectious virus with a long incubation period, encompassing asymptomatic infections. Therefore, accu...
Application Progress of Fluorescence Carbon Quantum Dots in Food Analysis
YUE Xiao-Yue , ZHOU Zi-Jun , WU Yong-Mei , LI Yan , LI Jin-Cai , BAI Yan-Hong , WANG Jian-Long
2020, 48(10): 1288-1295  doi: 10.19756/j.issn.0253-3820.201005
[Abstract](1033) [FullText HTML] [PDF 1887KB](19)
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As a novel class of luminescent nanomaterials, carbon quantum dots (CQDs) have attracted considerable interest in the field of food safety due to their high bright luminescence, low toxicity and good biocompatibility. This paper introduced the fluore...
A Novel Laser Ablation Chamber for Microwave Plasma Atomic Emission Spectrometry and Its Application
WANG Hao-Yu , CHEN Sha , YIN Peng-Kun , LIN Qing-Yu , WANG Xu , DUAN Yi-Xiang
2020, 48(10): 1296-1304  doi: 10.19756/j.issn.0253-3820.201238
[Abstract](1122) [FullText HTML] [PDF 2344KB](21)
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Microwave plasma torch atomic emission spectrometry (MPT-AES) is a widely used element detection method. However, MPT-AES requires complex digestion and desolvation treatment when detecting solid samples. After many improvements, a new Z-shaped laser...
Multi-parameter and Quantitative Characterization of Urinary Extracellular Vesicles at Single-Particle Level
LIU Hai-Sheng , YUAN Wen-Li , CHEN Yong-Yu , CHEN Chen , TIAN Ye , YAN Xiao-Mei
2020, 48(10): 1305-1314  doi: 10.19756/j.issn.0253-3820.201443
[Abstract](998) [FullText HTML] [PDF 5227KB](10)
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Urinary extracellular vesicles (uEVs) are a rising star in liquid biopsies and have shown great potential in disease diagnosis and treatment monitoring. However, knowledge about the size distribution, particle concentration and biochemical phenotype ...
Rapid Analysis of Bacteremia by Membrane Extraction Electrospray Ionization Mass Spectrometry
WANG Jiang , HUANG Li-Juan , XU Zhen-Jun , LIU Yu , XU Jing-Juan , CHEN Hong-Yuan
2020, 48(10): 1315-1324  doi: 10.19756/j.issn.0253-3820.201263
[Abstract](1138) [FullText HTML] [PDF 6260KB](9)
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A custom membrane extraction electrospray ionization mass spectrometry (MEESI-MS) method was developed to detect the representative bacteric metabolites and classify four kinds of bacteria including Escherichia coli, Staphylococcus aureus, Klebsiella...
Aggregation Induced Emission Fluorogen-based Label-free Biosensor for Highly Sensitive Detection of Carcinoembryonic Antigen
LI Hai-Yin , CHANG Jia-Fu , LYU Wen-Xin , LI Feng
2020, 48(10): 1325-1333  doi: 10.19756/j.issn.0253-3820.201295
[Abstract](859) [FullText HTML] [PDF 3594KB](6)
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A highly sensitive and label-free fluorescence carcinoembryonic antigen (CEA) biosensor was developed via target-triggered enzymatic recycling amplification reaction. In this strategy, aggregation induced emission fluorogen (AIEgen) TPE-M was chosen ...
A Preliminary Study of Human Urine Exosome Glycoproteome
WANG Shu-Yue , JIA Sheng-Nan , LI Xiao-Ying , ZHANG Hai-Yang , FENG Xin , WANG Shi , TAO Wei-Guo , HU Liang-Hai
2020, 48(10): 1334-1342  doi: 10.19756/j.issn.0253-3820.201220
[Abstract](844) [FullText HTML] [PDF 5139KB](8)
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Protein glycosylation of human urine exosome can reflect important biological information, which promotes a large-scale identification for the study of urine exosome glycoproteome. In this work, a two step enrichment method based on extracellular ves...
Study on Fast Recognition of Biotoxins and Biological Modifiers Using Data Fusion Algorithm
NAN Di-Na , LIU Wei-Wei , FU Wen-Xiang , LI Bao-Qiang , KONG Jing-Lin
2020, 48(10): 1343-1350  doi: 10.19756/j.issn.0253-3820.201137
[Abstract](911) [FullText HTML] [PDF 3635KB](8)
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A method for analysis of various biotoxins and biological modifiers based on electrospray ionization-ion mobility spectrometry (ESI-IMS) and Raman spectroscopy was established. A database of 26 kinds of white powders, including alkaloids such as acon...
Proteomics Analysis of Human Renal Clear Cell Carcinoma by Two MS2-based Label-free Approaches
GUO Jia , LIU Jin-Hua , YANG Zhao-Wei , LI Yi , HE Cheng-Yan
2020, 48(10): 1351-1358  doi: 10.19756/j.issn.0253-3820.201373
[Abstract](1063) [FullText HTML] [PDF 2841KB](6)
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Advanced renal cancer is not sensitive to chemotherapy or radiotherapy, and its effective treatment is very limited. At present, the recognition of specific and effective molecular targets for advanced renal cancer is far from enough. In this work, 2...
Correlation of Fluorescence Imaging with Inductively Coupled Plasma-Mass Spectrometry for Quantification of Intracellular Copper Nanoparticles
ZHENG An-Qi , HAO Ya-Nan , GUO Ting-Ting , SHU Yang , WANG Jian-Hua
2020, 48(10): 1359-1366  doi: 10.19756/j.issn.0253-3820.201270
[Abstract](929) [FullText HTML] [PDF 3500KB](14)
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A fluorescence imaging protocol was developed for the quantification analysis of intracellular glutathione-copper nanoparticles (GSH-CuNPs). After culturing MCF-7 cells with near infrared luminescent GSH-CuNPs, the fluorescence imaging of intracellul...
Broadband Terahertz Spectroscopy of Glutathione
GUO Cong , ZHU Zhong-Jie , SHEN Jian-Xiong , ZHANG Zong-Chang , ZHANG Jian-Bing , ZHAO De-Ming , ZHAO Hong-Wei
2020, 48(10): 1367-1374  doi: 10.19756/j.issn.0253-3820.201148
[Abstract](982) [FullText HTML] [PDF 3011KB](10)
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The conformation of glutathione is of great significance for its biological function. In this study, the absorption spectra of reduced (GSH) and oxidized (GSSG) glutathione in the frequency range of 0.5-12 THz were obtained by the air plasma terahert...
Preparation and Adsorption Performance of Core-Shell Magnetic Molecularly Imprinted Polymers for Estradiol
WANG Yue , GUO Kai-Chen , TIAN Xue-Meng , ZHAO Wen-Chang , Kaidiriye·Setiwalidi , GAO Rui-Xia
2020, 48(10): 1375-1382  doi: 10.19756/j.issn.0253-3820.201317
[Abstract](1264) [FullText HTML] [PDF 2915KB](9)
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The novel core-shell magnetic imprinted polymers (MIPs-E2) were prepared for selective adsorption of estradiol (E2) by template immobilization strategy, in which aniline (AN), ammonium persulfate (APS), E2 and carboxyl-modified Fe3O4 magnetic nanopar...
Spectroscopy and Molecular Docking Technique for Investigation of Interaction between Juglone and Human Serum Albumin
SHEN Bing-Jun , LIU Ting-Ting
2020, 48(10): 1383-1391  doi: 10.19756/j.issn.0253-3820.201281
[Abstract](1031) [FullText HTML] [PDF 4220KB](10)
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Juglone (Jug) is one of the main active substances in the folk anti-cancer prescription walnut green husk and there are few reports on its interaction with protein and its mechanism of action. Here, the interaction mechanism between Jug and human ser...
Magnetic Solid-phase Extraction Coupled with High-Performance Liquid Chromatography for Pesticide Residues Analysis in Citrus Sample
HUANG Si-Min , HU Yu-Fei , CHEN Yan-Long , LI Gong-Ke , XIA Ling
2020, 48(10): 1392-1399  doi: 10.19756/j.issn.0253-3820.201359
[Abstract](1039) [FullText HTML] [PDF 3059KB](33)
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The Fe3O4@COF with core-shell structures was synthesized and used as sorbent in magnetic solid-phase extraction (MSPE). This Fe3O4@COF-MSPE method coupled with high-performance liquid chromatography-ultraviolet detector (HPLC-UV) was employed for sim...
Determination of Phenolic Active Compounds in Light-aroma Baijiu by Solid Phase Extraction-High Performance Liquid Chromatography
WU Zi-Yang , HE Fei , LI He-He , SUN Jin-Yuan , SUN Xiao-Tao , SUN Bao-Guo
2020, 48(10): 1400-1408  doi: 10.19756/j.issn.0253-3820.191565
[Abstract](1034) [FullText HTML] [PDF 2056KB](23)
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An efficient and rapid method for qualitative and quantitative determination of ferulic acid, gallic acid, p-coumaric acid, syringic acid and (+)-catechin hydrate in baijiu by solid phase extraction-high performance liquid chromatography (SPE-HPLC) w...
Aptasensor Based on Nucleic Acid Base Quenching Fluorophore for Detection of Ochratoxin A
QU Yao , ZHANG Ya-Qi , XIAO Guang , YANG Cheng
2020, 48(10): 1409-1415  doi: 10.19756/j.issn.0253-3820.201260
[Abstract](869) [FullText HTML] [PDF 2071KB](11)
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Ochratoxin A (OTA) is one kind of mycotoxin, which is toxic and widely distributed. To avoid the poison of OTA, it is necessary to develop a rapid, low-cost and highly sensitive detection method to evaluate the concentration of OTA in the food. Here,...
Rapid Detection of Chemical Warfare Agent Simulants Using Gold Nanoparticles Substrate-assisted Enhanced Low Temperature Plasma-Mass Spectrometry
ZHANG Lin , MA Ming-Ying , LI Bao-Qiang , KONG Jing-Lin , ZHANG Si-Chun , ZHANG Xin-Rong
2020, 48(10): 1416-1421  doi: 10.19756/j.issn.0253-3820.201146
[Abstract](1509) [FullText HTML] [PDF 1787KB](11)
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The low temperature plasma (LTP) probe which can be used to quickly detect solid, liquid and gaseous samples has the characteristics of simple, fast and accurate, and easy to miniaturize. It has become a hot spot in the field of rapid detection of ch...
Non-continuous Industrial Data Analysis Using Discrete Fourier Transform
SUN Xue-Hui , ZHAO Bing , LUO Zhen , SUN Pei-Jian , PENG Bin , NIE Cong , SHAO Xue-Guang
2020, 48(10): 1422-1427  doi: 10.19756/j.issn.0253-3820.201158
[Abstract](1044) [FullText HTML] [PDF 1593KB](13)
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Analysis of big data is hot topic for exploring the contained values, however, the development of the analytical methods are still a challenging task due to the complexity in structure and variety. In this work, a method for pre-processing and modeli...
Investigation of Tandem Mass Spectrometric Behavior of Kaempferide by Online Collision Energy-resolved Mass Spectrometry-Structural Calculation
GUAN Peng-Wei , SONG Qing-Qing , ZHANG Ke , LI Ting , LI Wei , TU Peng-Fei , LI Jun , SONG Yue-Lin
2020, 48(10): 1428-1433  doi: 10.19756/j.issn.0253-3820.201192
[Abstract](1262) [FullText HTML] [PDF 1586KB](14)
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Regarding tandem mass spectrometry (MS/MS), the theory of the transition from precursor ion to product ion is actually chemical bond dissociation in gas phase resulted from the collision between ions and collision gas molecules with high energy. It i...
2020, 48(10): 1434-1437
[Abstract](660) [FullText HTML] [PDF 962KB](10)
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