2019 Volume 47 Issue 9

2019, 47(9):
[Abstract](2841) [FullText HTML] [PDF 3084KB](16)
Abstract:
Research and Application of Glycoprotein Sensors Based on Glycosyl Recognition
LIANG Jun-Yu , TONG Pei-Hong , LI Jian-Ping
2019, 47(9): 1283-1292  doi: 10.19756/j.issn.0253-3820.191270
[Abstract](1456) [FullText HTML] [PDF 1574KB](17)
Abstract:
Glycoprotein is a binding protein whose structure is mainly determined by the content of sugars and proteins and the peptide bonds to which it binds. The glycoproteins can be mainly classified into lectin, hormone, enzyme and immunoglobulin according...
New Methods for Cell Cycle Analysis
FANG Han-Shu , LANG Ming-Fei , SUN Jing
2019, 47(9): 1293-1301  doi: 10.19756/j.issn.0253-3820.191225
[Abstract](1717) [FullText HTML] [PDF 1727KB](24)
Abstract:
Cell cycle is a basic process during the cell life. Analyzing cell cycles can better understand the nature of diseases. Currently, cell cycles are mainly analyzed by flow cytometry, which investigate cell cycles by measuring intracellular DNA content...
Preparation of Tungstotellurate (Ⅵ)-coated Magnetic Nanoparticles for Separation and Purification of Ovalbumin in Egg White
CHE Zheng , YUE Shi-Yu , WU Nan , XU Jia-Wei , CHEN Qing
2019, 47(9): 1302-1308  doi: 10.19756/j.issn.0253-3820.191249
[Abstract](1649) [FullText HTML] [PDF 1451KB](11)
Abstract:
Tungstotellurate(Ⅵ)-coated magnetic nanoparticles(MNP) were prepared by immobilization of tungstotellurate (Ⅵ) salt onto the surface of branched polyethyleneimine (PEI)-modified magnetic nanoparticles via electrostatic interaction. The structure and ...
Real-Time Monitoring of Click Reaction Based on Confined Glass Nanopipette
LU Si-Min , YU Ru-Jia , LONG Yi-Tao
2019, 47(9): 1309-1313  doi: 10.19756/j.issn.0253-3820.191333
[Abstract](1454) [FullText HTML] [PDF 1263KB](23)
Abstract:
Click reaction is a reliable, powerful and selective method in material science, medical science and biochemistry. Monitoring of click reaction at single particle scale contributes to revealing anisotropy and mechanism of click reaction. In this stud...
Photoelectrochemical Biosensor for Detection of Mercury(Ⅱ) Based on CdS Quantum Dots/TiO2 Composite Material
ZHENG Shan , LIU Yang , CHEN Piao-Piao , XING Yi-Chen , ZHANG Wei-Bo , SHI Ya-Ying , HUANG Chao-Biao
2019, 47(9): 1314-1320  doi: 10.19756/j.issn.0253-3820.181818
[Abstract](1355) [FullText HTML] [PDF 2143KB](14)
Abstract:
A sensitive mercury ion (Hg2+) sensor was designed by using CdS QDs/TiO2 composite material as the photoelectric conversion unit. After the coupling of two inorganic semiconductors (CdS QDs and TiO2) with different band gaps, the performance of elect...
Influence of Geometric Configuration on Mixing Performance of Planar Chaotic Micro-mixer
LIU Zhao-Miao , ZHAO Sheng , WANG Wen-Kai , LI Meng-Qi , PANG Yan , YIN Shen , XU Ying-Li
2019, 47(9): 1321-1329  doi: 10.19756/j.issn.0253-3820.191275
[Abstract](1583) [FullText HTML] [PDF 2879KB](13)
Abstract:
As an important part of laboratory chip (LOC) and micro-total analysis system (μTAS), micro-mixer has been widely used in the fields of biological analysis and chemical synthesis, most of which are used in the pre-treatment part of the detection syst...
Molecularly Imprinted Photonic Hydrogels for Visual Detection of Methylanthranilate in Wine
WU Wei-Zhen , HUANG Meng-Xia , HUANG Qing-Da , LYU Cai-Hua , LAI Jia-Ping , SUN Hui
2019, 47(9): 1330-1336  doi: 10.19756/j.issn.0253-3820.191087
[Abstract](1446) [FullText HTML] [PDF 2150KB](10)
Abstract:
Methylanthranilate (MA) is a kind of spice illegally added in the wine in China. The determination of MA in wine is an important quality control link. Therefore, it is of great significance to develop a rapid method for detection of prohibited spice ...
Portable Glucose Meter for Detection of Mercury(Ⅱ) Ion
SHI Wei-Ping , CAI Jie , YANG Ya-Ni , LUO Huan-Huan , LIU Bing-Qian , FU Qiu-Ping
2019, 47(9): 1337-1343  doi: 10.19756/j.issn.0253-3820.191311
[Abstract](2872) [FullText HTML] [PDF 1970KB](23)
Abstract:
Based on the specific recognition of T-Hg2+-T mismatched for mercury ions (Hg2+), a simple and sensitive method for detecting Hg2+ was developed by using Fe3O4 nano-magnetic beads (MB) as reaction platform and portable blood glucose meter (PGM) as de...
Investigation of Characters of Bioaerosols on Single Particle Aerosol Mass Spectrometer
ZENG Zhen , YU Jia-Jun , LIU Ping , HUANG Fu-Gui , CHEN Ying , HUANG Zheng-Xu , GAO Wei , LI Mei , ZHOU Zhen , LI Lei
2019, 47(9): 1344-1351  doi: 10.19756/j.issn.0253-3820.191202
[Abstract](1399) [FullText HTML] [PDF 2081KB](11)
Abstract:
Active bioaerosols suspended in the atmosphere can harm human health through the human respiratory system, so the detection of active bioaerosols is extremely important. In recent years, more and more research has attempted to apply single particle a...
Investigation of Generation Method of Double Emulsion Broplets Based on Compositional Microfluidic Chip
WANG Meng , ZHU Li , XIAO Na , JI Cheng-Wei
2019, 47(9): 1352-1358  doi: 10.19756/j.issn.0253-3820.191347
[Abstract](1506) [FullText HTML] [PDF 3249KB](24)
Abstract:
A method was developed for producing double emulsion droplets based on a compositional microfluidic chip that was consisted of a T-micro connector, a cross-micro connector and a glass capillary. T-junction microfluidic chip could fabricate high throu...
Study on Fragmentation of Linear Alkanes in Proton-Transfer-Reaction Mass Spectrometry
SUN Yun , YANG Ru , SUN Chuan-Qiang , LIU Hai-Pei , WANG Yan , JIANG Xue-Hui
2019, 47(9): 1359-1365  doi: 10.19756/j.issn.0253-3820.191221
[Abstract](2043) [FullText HTML] [PDF 1506KB](16)
Abstract:
The pure standards of 10 linear alkanes (C6-C15) were detected by proton-transfer-reaction mass spectrometry (PTR-MS) through a self-made liquid sampling device. The experimental results showed that the fragmentation of linear alkanes in PTR-MS under...
Determination of Fluoride Ion in Environmental Water Based on Manganese-Doped Zinc Sulfide Quantum Dots/Europium (Ⅲ) Nanocomposites
SUN Xiao-Jie , MIAO Yan-Ming , LYU Jin-Zhi , LUO Shi-Qing , WANG Rui-Rui , YAN Gui-Qin
2019, 47(9): 1366-1372  doi: 10.19756/j.issn.0253-3820.181654
[Abstract](1355) [FullText HTML] [PDF 2914KB](9)
Abstract:
An optical sensing system for specific detection of F- was developed on the basis of room-temperature phosphorescence of manganese-doped Zinc sulfide quantum dots (Mn-ZnS QDs). In this system, Eu3+ bonded with Mn-ZnS QDs to form nanocomposites, and t...
A Sensitive Colorimetric Method for Detection of Chromium Ion(Ⅲ) Based on Surface Plasmon Resonance Effect of Gold Nanoparticles
ZHANG Teng , ZHONG Yi-Han , ZHANG Shou-Ting , DANG Xin-Yu , LU Xiao-Quan
2019, 47(9): 1373-1381  doi: 10.19756/j.issn.0253-3820.191306
[Abstract](1415) [FullText HTML] [PDF 2511KB](10)
Abstract:
A method was proposed for the effective and accurate colorimetric detection of ultra-trace trivalent chromium ions (Cr3+) in water based on citric acid-modified gold nanoparticles (CA-AuNPs). CA-AuNPs under different pH conditions were prepared by in...
Accurate Determination of Harmful and Doping Elements in Soft Magnetic Ferrite Powders Using Inductively Coupled Plasma Tandem Mass Spectrometry
FU Liang , SHI Shu-Yun , MA Jun-Cai
2019, 47(9): 1382-1389  doi: 10.19756/j.issn.0253-3820.191232
[Abstract](1307) [FullText HTML] [PDF 1517KB](9)
Abstract:
A new strategy for eliminating mass spectral interference in analysis of soft magnetic ferrite powders by inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) in the MS/MS mode using mixed reaction gases was proposed. The mixture of nitric...
Quantitative Analysis of Silver Nanoparticles in Single Cell by Laser Ablation Inductively Coupled Plasma-Mass Spectrometry
FU Dong-Xu , ZHENG Ling-Na , LIU Jin-Hui , WANG Bing , ZHU Yun , WANG Meng , FENG Wei-Yue
2019, 47(9): 1390-1394  doi: 10.19756/j.issn.0253-3820.191283
[Abstract](1305) [FullText HTML] [PDF 1084KB](9)
Abstract:
In recent years, single cell analysis has become one of hot topics in analytical chemistry. In this work, a method was developed for quantitative analysis of silver nanoparticles (AgNP) in single cells by laser ablation-inductively coupled plasma-mas...
Determination of Nitrite and Nitrate in Dairy Products by Protonated Chitosan Functionalized Gold Nanoarticles Coated Stir Bar Sorptive Extraction with Ion Chromatography
LIU Hai-Xia , DI Jing , RAO Hong-Hong , ZHENG Yan-Ping , GUO Jin-Xin , ZHAO Guo-Hu
2019, 47(9): 1395-1401  doi: 10.19756/j.issn.0253-3820.191277
[Abstract](1463) [FullText HTML] [PDF 1599KB](13)
Abstract:
Protonated chitosan (PCTS) modified gold nanoparticles (AuNPs) composites coating was fabricated using an etched stainless steel wire as a support on which AuNPs were first deposited, then after modified with CTS by self-assembly method and protonate...
Optimization of Sample Preparation Method for Intracellular Metabolites Metabonomics Analysis of Escherichia Coli
LI Yang , WANG Ji-Tong , LIU Xiao-Lu , TIAN Jing , JIA Zheng , XIAO Zhi-Ming , FAN Xia
2019, 47(9): 1402-1410  doi: 10.19756/j.issn.0253-3820.191172
[Abstract](1457) [FullText HTML] [PDF 1251KB](11)
Abstract:
A sample preparation method was developed to simulate the process of intracellular metabolites metabonomics analysis of Escherichia coli. The Escherichia coli cell was firstly quenched with cold sodium chloride solution (0.85%, precooled at -80℃ for ...
Study on Analytical Properties of Sunflower Seed Husk as a New Wooden Electrospray Ionization Material
LIU Zhe , GUO Yun-Long , XU Qing-Xuan , LIU Shu-Ying
2019, 47(9): 1411-1418  doi: 10.19756/j.issn.0253-3820.181712
[Abstract](1407) [FullText HTML] [PDF 1917KB](11)
Abstract:
A simple and economical type of electrospray ionization (ESI) technique was developed for loading and ionization of samples by use of disposable wooden tips of sunflower seed husk. Samples could be loaded by simply dipping the tip into sample solutio...
Selection of Nanobody and Recognition Mechanism between Nanobody and Parathion
ZHANG Yu-Qi , ZHANG Jin-Ru , WANG Feng , LI Jia-Dong , SI Rui , LYU Li-Shan , SHEN Yu-Dong , WANG Hong , HAMMOCK Bruce D , SUN Yuan-Ming
2019, 47(9): 1419-1426  doi: 10.19756/j.issn.0253-3820.181772
[Abstract](1634) [FullText HTML] [PDF 1638KB](11)
Abstract:
The organophosphorus pesticides are widely used in agriculture and food in China. Parathion, which is highly toxic and persistent, is one of the most widely used organophosphorus pesticides. Variable domain of the H chain of heavy-chain antibodies (V...
Establishment and Application of Lead Ion Functional Nucleic Acid Colorimetric Biosensor
LI Chen-Wei , LIN Sheng-Hao , DU Zai-Hui , SUN Chun-Yan , XU Wen-Tao
2019, 47(9): 1427-1432  doi: 10.19756/j.issn.0253-3820.191217
[Abstract](1397) [FullText HTML] [PDF 1649KB](8)
Abstract:
A method for visual detection of Pb2+ in water based on lead ion (Pb2+) functional nucleic acid colorimetric biosensor was developed. The specific recognition and cleavage of Pb2+ by GR-5 deoxyribozyme was carried out, and the cleavage products of GR...
Application of Ethylenediaminetetraacetic Acid Disodium Salt to High Precision Measurement of Isotope of Boron in Acid-soluble Fraction of Sediments
YANG Jian , MA Yun-Qi , LI Xing-Yi , LIU Yu-Xiu , HAN Feng-Qing , ZHANG Yan-Ling , Syed Asim Hussain , LI Hai-Jun
2019, 47(9): 1433-1439  doi: 10.19756/j.issn.0253-3820.191113
[Abstract](1543) [FullText HTML] [PDF 1034KB](14)
Abstract:
When nature sediments (e.g. clay/carbonate sediments) are dissolved by hydrochloric acid, a large number of metallic ions such as Ca, Mg, Fe, Al and rare earth elements are released. These metal ions make large amount of water-insoluble hydroxide pre...
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