A gold catalyst of Au/ pyrenyl-graphdiyne (Pyr-GDY) was prepared by anchoring small size of gold nanoparticles (Au NPs) on the surface of Pyr-GDY for electrocatalytic nitrogen reduction reaction (eNRR), in which Au NPs with a size of approximately 3.69 nm was evenly distributed on spongy-like porous Pyr-GDY. The catalyst exhibited a good electrocatalytic activity for N₂ reduction in a nitrogen-saturated electrolyte, with an ammonia yield of 32.1 μg·h^-1·mg_cat^-1 at -0.3 V (vs RHE), 3.5 times higher than that of Au/C (Au NPs anchored on carbon black). In addition, Au/Pyr-GDY showed a Faraday efficiency (FE) of 26.9% for eNRR, and a good catalysis durability for over 22 h.