氯噻啉胶体金增强免疫层析分析方法的建立

引用本文: 施海燕, 盛恩泽, 马明, 华修德, 王鸣华. 氯噻啉胶体金增强免疫层析分析方法的建立[J]. 分析化学, 2017, 45(3): 403-408. doi: 10.11895/j.issn.0253-3820.160730 shu

Citation: SHI Hai-Yan, SHENG En-Ze, MA Ming, HUA Xiu-De, WANG Ming-Hua. Development of an Enhanced Colloidal Gold Immunochromatographic Assay for Detection of Imidaclothiz[J]. Chinese Journal of Analytical Chemistry, 2017, 45(3): 403-408. doi: 10.11895/j.issn.0253-3820.160730 shu

氯噻啉胶体金增强免疫层析分析方法的建立

南京农业大学植物保护学院, 南京 210095
基金项目:
本文系国家自然科学基金项目(No.31301692)资助

摘要: 利用生物素-链霉亲和素的高亲和作用，研制了一种简便、快速、高灵敏的氯噻啉胶体金增强免疫层析分析方法(Gold immunochromatographic assay，GICA)。将氯噻啉抗体和生物素化DNA与13 nm胶体金双标记，将链霉亲和素与41 nm胶体金标记，制备了氯噻啉胶体金增强免疫层析试纸条。系统优化了试纸条的工作条件，并通过交叉反应、添加回收和高效液相色谱(High performance liquid chromatography，HPLC)验证评价GICA的敏感性、特异性、精确性和准确性。在最优条件下，试纸条可在10 min内实现可视化判读，检出限(LOD)为25 ng/mL，除与吡虫啉有较大交叉反应外，与其它类似化合物无交叉反应。氯噻啉在河水、大米、黄瓜、番茄、梨、甘蓝和苹果样品中的添加浓度为0.05，0.5和5 μg/g时，GICA的检测结果均符合添加水平。在未知浓度河水和梨样品的检测中，GICA的检测结果与HPLC方法相符。

关键词:

English

Development of an Enhanced Colloidal Gold Immunochromatographic Assay for Detection of Imidaclothiz

College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
Received Date: 28 September 2016
Revised Date: 23 December 2016
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 31301692)

Abstract: An enhanced gold immunochromatographic assay (GICA) with simplicity, rapidity and high sensitivity was developed to detect imidaclothiz by using the high affinity between biotin and streptavidin. The 13-nm AuNPs were double-labeled with anti-imidaclothiz antibody and biotinylated DNA, and the 41-nm AuNPs were labeled with streptavidin to prepare an enhanced gold immunochromatographic test strip for imidaclothiz. The working conditions of the strip were systematically optimized, and the sensitivity, specificity, precision and accuracy were assessed by testing the cross-reactivity (CR), spiked recovery and validation with high performance liquid chromatography (HPLC). Under the optimal conditions, the detection could be completed in 10 min with visual result, and the limit of detection (LOD) was 25 ng/mL. The analysis showed no cross-reactivity with analogues of imidaclothiz except for imidacloprid. The detection results of GICA agreed with the spiked concentrations of imidaclothiz at spiked levels of 0.05, 0.5 and 5 μg/g in river water, rice, cucumber, tomato, pear, cabbage and apple samples. The detection results of GICA for imidaclothiz in unknown concentration river water and pear samples were consistent with that of HPLC.

Key words:

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氯噻啉胶体金增强免疫层析分析方法的建立

English

Development of an Enhanced Colloidal Gold Immunochromatographic Assay for Detection of Imidaclothiz

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

氯噻啉胶体金增强免疫层析分析方法的建立

English

Development of an Enhanced Colloidal Gold Immunochromatographic Assay for Detection of Imidaclothiz

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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