钯纳米粒子修饰的三甲氧苄啶分子印迹膜传感器的制备及其识别性能研究

引用本文: 韦贻春, 余会成, 李浩, 陈其锋, 韦冬萍, 雷福厚, 谭学才. 钯纳米粒子修饰的三甲氧苄啶分子印迹膜传感器的制备及其识别性能研究[J]. 分析化学, 2017, 45(9): 1367-1374. doi: 10.11895/j.issn.0253-3820.170261 shu

Citation: WEI Yi-Chun, YU Hui-Cheng, LI Hao, CHEN Qi-Feng, WEI Dong-Ping, LEI Fu-Hou, TAN Xue-Cai. Preparation and Recognition Properties of Trimethoprim Sensor Based on Palladium Nanoparticles-modified Molecularly Imprinted Polymer[J]. Chinese Journal of Analytical Chemistry, 2017, 45(9): 1367-1374. doi: 10.11895/j.issn.0253-3820.170261 shu

钯纳米粒子修饰的三甲氧苄啶分子印迹膜传感器的制备及其识别性能研究

1.
广西民族大学化学化工学院, 广西林产化学与工程重点实验室, 南宁 530006;2 广西民族大学化学化工学院, 广西高校食品安全与药物分析化学重点实验室, 南宁 530006;3 广西民族大学化学化工学院, 广西多糖材料与改性重点实验室, 南宁 530006
基金项目:
本文系广西自然科学基金（No.2015GXNSFAA139040）、广西中青年教师基础能力提升项目（No.2017KY0171）和广西民族大学校级科研项目（No.2016MDYB023）资助

摘要: 采用N，N'-亚甲基双丙烯酰胺（MBA）为功能单体、钯纳米粒子为掺杂剂、马来松香丙烯酸乙二醇酯为交联剂，在玻碳电极上热聚合具有三甲氧苄啶（TMP）识别性能的钯纳米材料修饰的分子印迹传感膜。采用扫描电镜及红外光谱对合成的钯纳米材料、印迹传感膜的形貌及其结构进行了表征；采用循环伏安法（CV）、交流阻抗法（EIS）对钯纳米粒子掺杂的印迹电极与无掺杂电极的电化学性能进行了研究。结果表明，纳米粒子掺杂的印迹电极与无掺杂电极的表面形貌及电化学性能明显不同。差分脉冲伏安法（DPV）表征结果表明，TMP的浓度在5.0×10^-7-4.0×10^-3 mol/L范围内与脉冲峰电流呈良好的线性关系（R=0.9995），检出限为3.2×10^-8 mol/L（S/N=3）。此钯纳米粒子掺杂的印迹传感器具有较高的灵敏度。即时电流测定结果表明，新诺明（SMZ）、磺胺嘧啶（SDZ）、葡萄糖（Glu）、尿素（Urea）对三甲氧苄啶（TMP）的测定不产生干扰。将此印迹传感器用于实际样品中TMP的检测，加标回收率为96.8%~102.0%。

关键词:

English

Preparation and Recognition Properties of Trimethoprim Sensor Based on Palladium Nanoparticles-modified Molecularly Imprinted Polymer

1.
Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China;2 Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China;3 Guangxi Key Laboratory of Polysaccharide Materials and their Modification, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China
Received Date: 26 April 2017
Revised Date: 18 July 2017

Abstract: To improve the sensitivity of molecularly imprinted electrochemical sensors, a Pd nanoparticles-modified molecularly imprinted polymer (MIP) film for the determination of trimethoprim (TMP) was developed by thermal polymerization with N, N'-methylene diacrylamide as a functional monomer, Pd nanoparticle as a dopant and ethylene glycol maleic rosinate acrylate as a crosslinking agent. The morphologies and chemical structures of the Pd nano-materials and the imprinted films were characterized using Fourier transform infrared spectroscopy and scanning electron microscopy, respectively. The electrochemical properties of the nano-doped and undoped MIP sensors were investigated by cyclic voltammetry and electrochemical impedance spectroscopy. Results showed that the morphologies and chemical structures and the electrochemical properties of the doped molecularly imprinted sensor were remarkably different from those of the undoped imprinted sensor. Linear responses of the imprinted sensor to TMP were observed for concentrations ranging from 5.0×10^-7 mol/L to 4.0×10^-3 mol/L (R=0.9995), with a detection limit of 3.2×10^-8 mol/L (S/N=3). The Pd nanoparticle doped MIP sensors exhibited high selectivity. The chronoamperometry showed that no interference from potential interfering species such as sulfamethoxazole, sulfadiazine, glucose, and urea were noted. The proposed electrochemical sensor was used to determine TMP in actual samples, with average recoveries of 96.8%-102.0%.

Key words:

Palladium nanoparticles
/ Trimethoprim
/ Sensor
/ Molecularly imprinted polymer
/ Ethylene glycol maleic rosinate acrylate

1. [1]
  Phu T M, Scippo M L, Phuong N T, Cao T K T, Tien C T K, Son C H, Dalsgaard A. Aquaculture,2015, 437:256-262
2. [2]
  Liguoro M D, Leva V D, Bona M D, Merlanti R, Caporale G, Radaelli G. Ecotoxicol. Environ. Saf.,2012, 82:114-121
3. [3]
  ZHANG Hong-Wu, GUO Jian-Wen, LI Kang, WANG Wei-Sheng, WU Yu-Ping, LU Qi-Wen, ZAI Hai-Yun. Chinese J. Anal. Chem.,2012, 40(5):699-704 张红武, 郭建文,李康, 王伟声, 吴玉萍, 卢绮雯, 翟海云.分析化学,2012, 40(5):699-704
4. [4]
  Riad S M, Salem H, Elbalkiny H T, Khattab F I. Spectrochim. Acta.,2015, 140:451-461
5. [5]
  Robert C, Gillard N, Brasseur P Y, Ralet N, Dubois M, Delahaut P. Food Control,2015, 50:509-515
6. [6]
  Wang C, Wang X, Xu X, Liu B, Xu X, Sun L, Li H, Zhang J. Anal. Chim. Acta,2016, 909:75-83
7. [7]
  Gibbons S E, Wang C, Ma Y. Talanta,2011, 84(4):1163-1168
8. [8]
  Pereira P F, Silva W P D, Muñoz R A A, Richter E M. J. Electroanal. Chem.,2016, 766:87-93
9. [9]
  Sgobbi L F, Razzino C A, Machado S A S. Electrochim. Acta,2016, 191:1010-1017
10. [10]
  Kupis-Rozmysłowicz J, Wagner M, Bobacka J, Lewenstam A, Migdalski J. Electrochim. Acta,2016, 188:537-544
11. [11]
  YU Hui-Cheng, HUANG Xue-Yi, TAN Xue-Cai, LEI Fu-Hou, MAO Hui-Juan. Journal of Instrumental Analysis, 2014,33(7):752-757 余会成, 黄学艺, 谭学才, 雷福厚, 毛慧娟.分析测试学报,2014,33(7):752-757
12. [12]
  Prasad B B, Singh K. Electrochim. Acta,2016, 187:193-203
13. [13]
  Rezaei B, Boroujeni M K, Ensafi A A. Electrochim. Acta,2014, 123:332-339
14. [14]
  Sharma P S, Dabrowski M, D'Souza F, Kutner W. TRAC-Trends Anal. Chem.,2013, 51(11):146-157
15. [15]
  QI Yu-Bing, LIU Ying, SONG Qi-Jun. Chinese J. Anal. Chem.,2011, 39(7):1053-1057 齐玉冰, 刘瑛, 宋启军.分析化学,2011, 39(7):1053-1057
16. [16]
  Patra S, Roy E, Madhuri R, Sharma P K. Anal. Chim. Acta,2016, 918:77-88
17. [17]
  Yu D, Zeng Y, Qi Y, Zhou T, Shi G. Biosens. Bioelectron.,2012, 38(1):270-277
18. [18]
  Li J, Yin W, Tan Y, Pan H. Sens.Actuators B,2014, 197(3):109-115
19. [19]
  Aziz M A, Kawde A N. Microchim. Acta,2013, 180:837-843
20. [20]
  Chen X, Pan H, Liu H, Du M. Electrochim. Acta,2010, 56:636-643
21. [21]
  LEI Fu-Hou, ZHAO Kang, LI Xiao-Yan, LU Jian-Fang, GUAN Yu-Ting. Fine Chem.,2010, 27(1):11-15, 100 雷福厚, 赵慷, 李小燕, 卢建芳, 关瑜婷.精细化工,2010, 27(1):11-15, 100
22. [22]
  Amin R S, Hameed R M A, El-Khatib K M. Appl. Catal. B,2014, 148-149(6):557-567
23. [23]
  Lopez C, Claude B, Morin Ph, Max J P, Pena R, Ribet J P. Anal. Chim. Acta,2011, 683(2):198-205
24. [24]
  Yu H C, Wei Y C, Lei F H, Tan X C, Li H, Chen Q F. Prog. Org. Coat.,2015, 82:41-45
25. [25]
  LI Jian-Wen, WEI Shou-Lian, JIANG Jie-Tao. Chinese J. Anal. Chem.,2016, 44(1):103-109 利健文, 韦寿莲, 江杰涛.分析化学,2016, 44(1):103-109
26. [26]
  LIN Yu-Lin, LI Xiao-Juan, ZHANG Xin-Ying, CAO Jie, ZHANG Jian-Hua, CHEN Xiao-Ying. Chinese J. Anal. Chem.,2016, 44(10):1547-1554 林瑜琳, 李小娟, 张新颖, 曹洁, 张建华, 陈小英.分析化学,2016, 44(10):1547-1554
27. [27]
  ZHANG Chun-Yan, WANG Pei-Long, SHI Lei, SU Xiao-Ou. Chinese J. Anal. Chem.,2016, 44(12):1859-1866 张春艳, 王培龙, 石雷, 苏晓鸥.分析化学,2016, 44(12):1859-1866
28. [28]
  Silva H D, Pacheco J G, Magalhães J M, Viswanathan S, Delerue-Matos C. Biosens. Bioelectron.,2014, 52(2):56-61

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沈阳化工大学材料科学与工程学院沈阳 110142

钯纳米粒子修饰的三甲氧苄啶分子印迹膜传感器的制备及其识别性能研究

English

Preparation and Recognition Properties of Trimethoprim Sensor Based on Palladium Nanoparticles-modified Molecularly Imprinted Polymer

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

钯纳米粒子修饰的三甲氧苄啶分子印迹膜传感器的制备及其识别性能研究

English

Preparation and Recognition Properties of Trimethoprim Sensor Based on Palladium Nanoparticles-modified Molecularly Imprinted Polymer

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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