鸡肉样品中痕量喹诺酮类抗生素的表面增强拉曼光谱检测研究

引用本文: 徐婧, 郑红, 谢丽芳, 林伟琦, 高静, 谢泽中, 陈宏炬, 曾勇明, 刘国坤, 田中群. 鸡肉样品中痕量喹诺酮类抗生素的表面增强拉曼光谱检测研究[J]. 分析化学, 2023, 51(3): 397-404. doi: 10.19756/j.issn.0253-3820.221227 shu

Citation: XU Jing, ZHENG Hong, XIE Li-Fang, LIN Wei-Qi, GAO Jing, XIE Ze-Zhong, CHEN Hong-Ju, ZENG Yong-Ming, LIU Guo-Kun, TIAN Zhong-Qun. Fast Detection of Trace Enrofloxacin and Ciprofloxacin in Chicken Meat by Surface-enhanced Raman Spectroscopy[J]. Chinese Journal of Analytical Chemistry, 2023, 51(3): 397-404. doi: 10.19756/j.issn.0253-3820.221227 shu

鸡肉样品中痕量喹诺酮类抗生素的表面增强拉曼光谱检测研究

徐婧 ^1, ,
郑红 ^1, ,
谢丽芳 ^1, ,
林伟琦 ^3, ,
高静 ^3, ,
谢泽中 ^2, ,
陈宏炬 ^4, ,
曾勇明 ^4, ,
刘国坤 ^{1,
,} ,
田中群 ^2,

1.
厦门大学环境与生态学院, 近海海洋环境科学国家重点实验室, 厦门 361102;
2.
厦门大学化学化工学院, 固体表面物理化学国家重点实验室, 厦门 361005;
3.
厦门市产品质量监督检验院, 厦门 361000;
4.
厦门市普识纳米科技有限公司, 厦门 361000

通讯作者: 刘国坤,E-mail:guokunliu@xmu.edu.cn

基金项目:
国家自然科学基金项目(No.41876099)、国家重点研发计划(No.2018YFC1602600)和厦门市科技计划(No.3502Z20183002)资助。

摘要: 随着人们对食品品质要求日益增高,食品中痕量违禁物质的快速定性和定量分析成为食品安全领域的热点。本研究基于表面增强拉曼光谱(Surface-enhanced Raman spectroscopy,SERS)技术,结合自主研发的快速前处理试剂盒,建立了畜牧养殖等行业中广泛关注的2种喹诺酮类抗生素恩诺沙星和环丙沙星的现场快速检测方法。利用本方法检测了5种市售鸡肉(3种乌鸡肉、2种白条鸡肉)中的恩诺沙星和环丙沙星,结果表明,与标准方法高效液相色谱-串联质谱(High-performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)相比,所建立的SERS方法定性准确(可有效鉴定2种结构相似的喹诺酮抗生素)、定量可靠(误差在8.2%~14.4%范围内)、灵敏度高(最低可检出浓度在100 ng/L水平),可满足食品质量控制要求。本研究建立的SERS检测方法实用性强、操作简便快速,可用于实际样品中喹诺酮类药物的快速筛查。

关键词:

English

Fast Detection of Trace Enrofloxacin and Ciprofloxacin in Chicken Meat by Surface-enhanced Raman Spectroscopy

1.
State Key Laboratory of Marine Environmental Science, College of the Environment and Ecology, Xiamen University, Xiamen 361102, China;
2.
State Key Laboratory of Physical Chemistry of Solid Surface, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;
3.
Market and Quality Supervision Commission of Xiamen Municipality, Xiamen 361000, China;
4.
Xiamen Perser Nano Technology Co. Ltd., Xiamen 361000, China

Corresponding author: LIU Guo-Kun, guokunliu@xmu.edu.cn

Received Date: 09 May 2022
Revised Date: 14 October 2022
Fund Project:
Supported by the National Natural Science Foundation of China (No. 41876099), the National Key Research & Development Program of China (No. 2018YFC1602600) and the Xiamen Science and Technology Project (No. 3502Z20183002).

Abstract: With the increasing public concern on food quality, especially the zero-tolerance of illegal additives, the rapid qualitative and quantitative analysis of trace banned substances in food is in a prosperity stage in the field of food safety. Here, by combining with a self-developed rapid pretreatment kit, an on-site, rapid and sensitive detection strategy for trace antibiotics such as enrofloxacin and ciprofloxacin, two typical abused antibiotics in livestock and other industries, by surface-enhanced Raman spectroscopy (SERS) technique was developed. The abused quinolones residues in chicken meat (3 kinds of silky chicken meat and 2 kinds of plain chicken meat) were successfully screened by the developed SERS method, which showed an accurate qualification and a reliable quantitation in comparison with that by standard high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technique. The proposed SERS-based strategy may provide a practical and simple method for rapid detection of other quinolones in real samples.

Key words:

1. [1]
  BETITRA Y, TERESA V, MIGUEL V, ABDELAZIZ T. Asian Pac. J. Tropical Med., 2014, 7(6):462-467.BETITRA Y, TERESA V, MIGUEL V, ABDELAZIZ T. Asian Pac. J. Tropical Med., 2014, 7(6):462-467.
2. [2]
  ZHANG L, ADDLA D, PONMANI J, WANG A, XIE D, WANG Y N, ZHANG S L, GENG R X, CAI G X, LI S, ZHOU C H. Eur. J. Med. Chem., 2016, 111:160-182.ZHANG L, ADDLA D, PONMANI J, WANG A, XIE D, WANG Y N, ZHANG S L, GENG R X, CAI G X, LI S, ZHOU C H. Eur. J. Med. Chem., 2016, 111:160-182.
3. [3]
  WIMALASENA S H M P, SHIN G W, HOSSAIN S, HEO G J. J. Vet. Med. Sci., 2017, 79(5):921-926.WIMALASENA S H M P, SHIN G W, HOSSAIN S, HEO G J. J. Vet. Med. Sci., 2017, 79(5):921-926.
4. [4]
  RAJALAKSHMI K, GUNASEKARAN S, KUMARESAN S. Indian J. Phys., 2014, 88(7):733-744.RAJALAKSHMI K, GUNASEKARAN S, KUMARESAN S. Indian J. Phys., 2014, 88(7):733-744.
5. [5]
  WALSH C. Antibiotics:Actions, Origins, Resistance. Washington, D. C, ASM Press, 2003.WALSH C. Antibiotics:Actions, Origins, Resistance. Washington, D. C, ASM Press, 2003.
6. [6]
  SEO K W, LEE Y J. Poult. Sci., 2019, 98(6):2700-2701.SEO K W, LEE Y J. Poult. Sci., 2019, 98(6):2700-2701.
7. [7]
  CAMPOLIRICHARDS D M, MONK J P, PRICE A, BENFIELD P, TODD P A, WARD A. Drugs, 1988, 35(4):373-447.CAMPOLIRICHARDS D M, MONK J P, PRICE A, BENFIELD P, TODD P A, WARD A. Drugs, 1988, 35(4):373-447.
8. [8]
  MATHEW A G, CISSELL R, LIAMTHONG S. Foodborne Pathog. Dis., 2007, 4(2):115-133.MATHEW A G, CISSELL R, LIAMTHONG S. Foodborne Pathog. Dis., 2007, 4(2):115-133.
9. [9]
  KOVALAKOVA P, CIZMAS L, MCDONALD T J, MARSALEK B, FENG M, SHARMA V K. Chemosphere, 2020, 251:126351.KOVALAKOVA P, CIZMAS L, MCDONALD T J, MARSALEK B, FENG M, SHARMA V K. Chemosphere, 2020, 251:126351.
10. [10]
  SMITH K E, BESSER J M, HEDBERG C W, LEANO F T, BENDER J B, WICKLUND J H, JOHNSON B P, MOORE K A, OSTERHOLM M T. N. Engl. J. Med., 1999, 340(20):1525-1532.SMITH K E, BESSER J M, HEDBERG C W, LEANO F T, BENDER J B, WICKLUND J H, JOHNSON B P, MOORE K A, OSTERHOLM M T. N. Engl. J. Med., 1999, 340(20):1525-1532.
11. [11]
  Ellenbergs. Food and Drug Administration (FDA). Encyclopedia of Biostatistics, 2005.Ellenbergs. Food and Drug Administration (FDA). Encyclopedia of Biostatistics, 2005.
12. [12]
  European Commission. Regulation (EC) No 810/2009 of the European Parliament and of the Council. 2009:810.European Commission. Regulation (EC) No 810/2009 of the European Parliament and of the Council. 2009:810.
13. [13]
  GB/T 31650-2019. National Food Safety Standard-Maximum Residue Limits for Veterinary Drugs in Foods. National Standards of the People's Republic of China. 食品安全国家安全标准-食品中兽药最大残留限量. 中华人民共和国国家标准. GB/T 31650-2019.
14. [14]
  PEIXOTO P S, TÓTH I V, SEGUNDO M A, LIMA J L F C. Int. J. Environ. Anal. Chem., 2016, 96(2):185-202.PEIXOTO P S, TÓTH I V, SEGUNDO M A, LIMA J L F C. Int. J. Environ. Anal. Chem., 2016, 96(2):185-202.
15. [15]
  PÉREZ-FERNÁNDEZ V, DOMÍNGUEZ-VEGA E, CREGO A L, GARCÍA M Á, MARINA M L. Electrophoresis, 2012, 33(1):127-146.PÉREZ-FERNÁNDEZ V, DOMÍNGUEZ-VEGA E, CREGO A L, GARCÍA M Á, MARINA M L. Electrophoresis, 2012, 33(1):127-146.
16. [16]
  BEIER R C, STANKER L H. Anal. Chim. Acta, 2001, 444(1):61-67.BEIER R C, STANKER L H. Anal. Chim. Acta, 2001, 444(1):61-67.
17. [17]
  LAN L, YAO Y, PING J, YING Y. Biosens. Bioelectron., 2017, 91:504-514.LAN L, YAO Y, PING J, YING Y. Biosens. Bioelectron., 2017, 91:504-514.
18. [18]
  LIU G, ZHENG H, LU J. Trends Environ. Anal. Chem., 2017, 16:16-23.LIU G, ZHENG H, LU J. Trends Environ. Anal. Chem., 2017, 16:16-23.
19. [19]
  MENG L, ZHANG W, MENG P, ZHU B, ZHENG K. J. Chromatogr. B, 2015, 989:46-53.MENG L, ZHANG W, MENG P, ZHU B, ZHENG K. J. Chromatogr. B, 2015, 989:46-53.
20. [20]
  GUIDI L R, SANTOS F A, RIBEIRO A C S R, FERNANDES C, SILVA L H M, GLORIA M B A. Food Chem., 2018, 245:1232-1238.GUIDI L R, SANTOS F A, RIBEIRO A C S R, FERNANDES C, SILVA L H M, GLORIA M B A. Food Chem., 2018, 245:1232-1238.
21. [21]
  MARTINS M T, BARRETO F, HOFF R B, JANK L, ARSAND J B, FEIJO T C, SCHERMAN S E E. Food Addit. Contam., Part A, 2015, 32(3):333-341.MARTINS M T, BARRETO F, HOFF R B, JANK L, ARSAND J B, FEIJO T C, SCHERMAN S E E. Food Addit. Contam., Part A, 2015, 32(3):333-341.
22. [22]
  AMELIN V G, VOLKOVA N M, TIMOFEEV A A, TRET'YAKOV A V. J. Anal. Chem., 2015, 70(9):1076-1084.AMELIN V G, VOLKOVA N M, TIMOFEEV A A, TRET'YAKOV A V. J. Anal. Chem., 2015, 70(9):1076-1084.
23. [23]
  GB/T 21312-2007. Analysis of Fourteen Quinolones in Food of Animal Origin by High Performance Liquid Chromatography Tandem Mass Spectrometry. National Standards of the People's Republic of China. 动物源性食品中14种喹诺酮药物残留检测方法液相色谱-质谱/质谱法.中华人民共和国国家标准. GB/T 21312-2007.
24. [24]
  XIE W, SCHLÜCKER S. Rep. Prog. Phys., 2014, 77(11):116502.XIE W, SCHLÜCKER S. Rep. Prog. Phys., 2014, 77(11):116502.
25. [25]
  CHOI I J. Nanoscience, 2016, 16(5):4274-4283.CHOI I J. Nanoscience, 2016, 16(5):4274-4283.
26. [26]
  WEI H, HOSSEIN ABTAHI S M, VIKESLAND P J. Environ. Sci.:Nano, 2015, 2(2):120-135.WEI H, HOSSEIN ABTAHI S M, VIKESLAND P J. Environ. Sci.:Nano, 2015, 2(2):120-135.
27. [27]
  GRAHAM D, MOSKOVITS M, TIAN Z Q. Chem. Soc. Rev., 2017, 46(13):3864-3865.GRAHAM D, MOSKOVITS M, TIAN Z Q. Chem. Soc. Rev., 2017, 46(13):3864-3865.
28. [28]
  BARANSKA M. Optical Spectroscopy and Computational Methods in Biology and Medicine. Springer Netherlands, 2014.BARANSKA M. Optical Spectroscopy and Computational Methods in Biology and Medicine. Springer Netherlands, 2014.
29. [29]
  SCHLUECKER S. Cheminform, 2014, 45(28):4756-4795.SCHLUECKER S. Cheminform, 2014, 45(28):4756-4795.
30. [30]
  LANGER J, JIMENEZ DE ABERASTURI D, AIZPURUA J, ALVAREZ-PUEBLA R A, AUGUIÉ B, BAUMBERG J J, BAZAN G C, BELL S E J, BOISEN A, BROLO A G, CHOO J, CIALLA-MAY D, DECKERT V, FABRIS L, FAULDS K, GARCÍA DE ABAJO F J, GOODACRE R, GRAHAM D, HAES A J, HAYNES C L, HUCK C, ITOH T, KÄLL M, KNEIPP J, KOTOV N A, KUANG H, LE RU E C, LEE H K, LI J F, LING X Y, MAIER S A, MAYERHÖFER T, MOSKOVITS M, MURAKOSHI K, NAM J M, NIE S, OZAKI Y, PASTORIZA-SANTOS I, PEREZ-JUSTE J, POPP J, PUCCI A, REICH S, REN B, SCHATZ G C, SHEGAI T, SCHLÜCKER S, TAY L L, THOMAS K G, TIAN Z Q, VAN DUYNE R P, VO-DINH T, WANG Y, WILLETS K A, XU C, XU H, XU Y, YAMAMOTO Y S, ZHAO B, LIZ-MARZÁN L M. ACS Nano, 2020, 14(1):28-117.LANGER J, JIMENEZ DE ABERASTURI D, AIZPURUA J, ALVAREZ-PUEBLA R A, AUGUIÉ B, BAUMBERG J J, BAZAN G C, BELL S E J, BOISEN A, BROLO A G, CHOO J, CIALLA-MAY D, DECKERT V, FABRIS L, FAULDS K, GARCÍA DE ABAJO F J, GOODACRE R, GRAHAM D, HAES A J, HAYNES C L, HUCK C, ITOH T, KÄLL M, KNEIPP J, KOTOV N A, KUANG H, LE RU E C, LEE H K, LI J F, LING X Y, MAIER S A, MAYERHÖFER T, MOSKOVITS M, MURAKOSHI K, NAM J M, NIE S, OZAKI Y, PASTORIZA-SANTOS I, PEREZ-JUSTE J, POPP J, PUCCI A, REICH S, REN B, SCHATZ G C, SHEGAI T, SCHLÜCKER S, TAY L L, THOMAS K G, TIAN Z Q, VAN DUYNE R P, VO-DINH T, WANG Y, WILLETS K A, XU C, XU H, XU Y, YAMAMOTO Y S, ZHAO B, LIZ-MARZÁN L M. ACS Nano, 2020, 14(1):28-117.
31. [31]
  SHI Q, HUANG J, SUN Y, YIN M, HU M, HU X, ZHANG Z, ZHANG G. Spectrochim. Acta, Part A, 2018, 197:107-113.SHI Q, HUANG J, SUN Y, YIN M, HU M, HU X, ZHANG Z, ZHANG G. Spectrochim. Acta, Part A, 2018, 197:107-113.
32. [32]
  TENG P, GAO D, YANG X, LUO M, KONG D, GAO S, LIU Z, LI Z, WEN X, YUAN L, LI K, BOWKETT M, COPNER N. Appl. Opt., 2021, 60(22):6659-6664.TENG P, GAO D, YANG X, LUO M, KONG D, GAO S, LIU Z, LI Z, WEN X, YUAN L, LI K, BOWKETT M, COPNER N. Appl. Opt., 2021, 60(22):6659-6664.
33. [33]
  ANDREOU C, MIRSAFAVI R, MOSKOVITS M, MEINHART C D. Analyst, 2015, 140(15):5003-5005.ANDREOU C, MIRSAFAVI R, MOSKOVITS M, MEINHART C D. Analyst, 2015, 140(15):5003-5005.
34. [34]
  XU Y, DU Y, LI Q, WANG X, PAN Y, ZHANG H, WU T, HU H. Food Anal. Methods, 2014, 7(6):1219-1228.XU Y, DU Y, LI Q, WANG X, PAN Y, ZHANG H, WU T, HU H. Food Anal. Methods, 2014, 7(6):1219-1228.
35. [35]
  ZHANG Y, TENG Y, QIN Y, REN Z, WANG Z. Anal. Lett., 2020, 53(4):660-670.ZHANG Y, TENG Y, QIN Y, REN Z, WANG Z. Anal. Lett., 2020, 53(4):660-670.
36. [36]
  ZHANG Y, HUANG Y, ZHAI F, DU R, LIU Y, LAI K. Food Chem., 2012, 135(2):845-850.ZHANG Y, HUANG Y, ZHAI F, DU R, LIU Y, LAI K. Food Chem., 2012, 135(2):845-850.
37. [37]
  LEE P C, MEISEL D. J. Phys. Chem., 1982, 86(17):3391-3395.LEE P C, MEISEL D. J. Phys. Chem., 1982, 86(17):3391-3395.
38. [38]
  FRENS G. Nat. Phys., 1973, 241(105):20-22.FRENS G. Nat. Phys., 1973, 241(105):20-22.
39. [39]
  ZHOU Z, ZHENG H, LIU T, XIE Z, LUO S, CHEN G, TIAN Z, LIU G. Anal. Chem., 2021, 93(24):8603-8612.ZHOU Z, ZHENG H, LIU T, XIE Z, LUO S, CHEN G, TIAN Z, LIU G. Anal. Chem., 2021, 93(24):8603-8612.
40. [40]
  GARRIDO C, AGUAYO T, CLAVIJO E, GÓMEZ-JERIA J S, CAMPOS-VALLETTE M M. J. Raman Spectrosc., 2013, 44(8):1105-1110.GARRIDO C, AGUAYO T, CLAVIJO E, GÓMEZ-JERIA J S, CAMPOS-VALLETTE M M. J. Raman Spectrosc., 2013, 44(8):1105-1110.
41. [41]
  HAN S, HONG S, LI X. J. Colloid Interface Sci., 2013, 410:74-80.HAN S, HONG S, LI X. J. Colloid Interface Sci., 2013, 410:74-80.
42. [42]
  ELIASSON C, LORéN A, ENGELBREKTSSON J, JOSEFSON M, ABRAHAMSSON J, JOHANSSON M, ABRAHAMSSON K. Anal. Chem., 2004, 61(4):755-760.ELIASSON C, LORéN A, ENGELBREKTSSON J, JOSEFSON M, ABRAHAMSSON J, JOHANSSON M, ABRAHAMSSON K. Anal. Chem., 2004, 61(4):755-760.
43. [43]
  HIDI I J, HEIDLER J, WEBER K, CIALLA-MAY D, POPP J. Anal. Bioanal. Chem., 2016, 408(29):8393-8401.HIDI I J, HEIDLER J, WEBER K, CIALLA-MAY D, POPP J. Anal. Bioanal. Chem., 2016, 408(29):8393-8401.
44. [44]
  LU J, CAI Z, ZOU Y, WU D, WANG A, CHANG J, WANG F, TIAN Z, LIU G. ACS Appl. Nano Mater., 2019, 2(10):6592-6601.LU J, CAI Z, ZOU Y, WU D, WANG A, CHANG J, WANG F, TIAN Z, LIU G. ACS Appl. Nano Mater., 2019, 2(10):6592-6601.
45. [45]
  XIE L, LU J, LIU T, CHEN G, LIU G, REN B, TIAN Z. J. Phys. Chem. Lett., 2020, 11(3):1022-1029.XIE L, LU J, LIU T, CHEN G, LIU G, REN B, TIAN Z. J. Phys. Chem. Lett., 2020, 11(3):1022-1029.
46. [46]
  MARSICH L, BONIFACIO A, MANDAL S, KROL S, BELEITES C, SERGO V. Langmuir, 2012, 28(37):13166-13171.MARSICH L, BONIFACIO A, MANDAL S, KROL S, BELEITES C, SERGO V. Langmuir, 2012, 28(37):13166-13171.
47. [47]
  ZHANG M, ZHOU Z, XU J, WANG W, PU S, HU W, LUO P, TIAN Z, GONG Z, LIU G. Spectrochim. Acta, Part A, 2022, 278:121365.ZHANG M, ZHOU Z, XU J, WANG W, PU S, HU W, LUO P, TIAN Z, GONG Z, LIU G. Spectrochim. Acta, Part A, 2022, 278:121365.

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

鸡肉样品中痕量喹诺酮类抗生素的表面增强拉曼光谱检测研究

通讯作者: 刘国坤,E-mail:guokunliu@xmu.edu.cn

English

Fast Detection of Trace Enrofloxacin and Ciprofloxacin in Chicken Meat by Surface-enhanced Raman Spectroscopy

Corresponding author: LIU Guo-Kun, guokunliu@xmu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

鸡肉样品中痕量喹诺酮类抗生素的表面增强拉曼光谱检测研究

通讯作者: 刘国坤,E-mail:guokunliu@xmu.edu.cn

English

Fast Detection of Trace Enrofloxacin and Ciprofloxacin in Chicken Meat by Surface-enhanced Raman Spectroscopy

Corresponding author: LIU Guo-Kun, guokunliu@xmu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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