Use of Capillary Electrophoresis in the Study of Interaction between dsDNA and Drug Molecules

引用本文: Juan Fang WU, Ling Xin CHEN, Guo An LUO, Yong Suo LIU, Yi Ming WANG, Zhi Hong JIANG. Use of Capillary Electrophoresis in the Study of Interaction between dsDNA and Drug Molecules[J]. Chinese Chemical Letters, 2006, 17(3): 383-386. shu

Citation: Juan Fang WU, Ling Xin CHEN, Guo An LUO, Yong Suo LIU, Yi Ming WANG, Zhi Hong JIANG. Use of Capillary Electrophoresis in the Study of Interaction between dsDNA and Drug Molecules[J]. Chinese Chemical Letters, 2006, 17(3): 383-386. shu

Use of Capillary Electrophoresis in the Study of Interaction between dsDNA and Drug Molecules

1.
Department of Chemistry, Tsinghua University, Beijing 100084;
2.
School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong
基金项目:
This work was supported by NNSF of China (No. 20475031), National "863" Project (No. 2002AA2Z2004) and Postdoctoral Science Foundation of China (No. 20040350303).

摘要: Two 17-mer dsDNA with different sequence characteristics were designed to investigate the binding characteristics of berberine, an anticancer drug with uncertain binding mode, and Hoechst 33258, a model DNA minor groove binder, with dsDNA, respectively by the capillary zone electrophoresis (CZE). Kenndler model analysis revealed that Hoechst 33258 exhibited intermediate affinity with dsDNA, while there was only low affinity and some weak binding preference for AATT-containing to GGCC-containing dsDNA for berberine.

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English

Use of Capillary Electrophoresis in the Study of Interaction between dsDNA and Drug Molecules

1.
Department of Chemistry, Tsinghua University, Beijing 100084;
2.
School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong
Received Date: 22 August 2005
Fund Project:
This work was supported by NNSF of China (No. 20475031), National "863" Project (No. 2002AA2Z2004) and Postdoctoral Science Foundation of China (No. 20040350303).

Abstract: Two 17-mer dsDNA with different sequence characteristics were designed to investigate the binding characteristics of berberine, an anticancer drug with uncertain binding mode, and Hoechst 33258, a model DNA minor groove binder, with dsDNA, respectively by the capillary zone electrophoresis (CZE). Kenndler model analysis revealed that Hoechst 33258 exhibited intermediate affinity with dsDNA, while there was only low affinity and some weak binding preference for AATT-containing to GGCC-containing dsDNA for berberine.

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

Use of Capillary Electrophoresis in the Study of Interaction between dsDNA and Drug Molecules

English

Use of Capillary Electrophoresis in the Study of Interaction between dsDNA and Drug Molecules

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Use of Capillary Electrophoresis in the Study of Interaction between dsDNA and Drug Molecules

English

Use of Capillary Electrophoresis in the Study of Interaction between dsDNA and Drug Molecules

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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