基于镍基纳米材料电化学传感器的α-糖苷酶抑制剂筛选方法研究

引用本文: 杨剑萍, 朱彩君, 李秋娜, 陈华莹, 黄智轩, 刘智康, 梁潇, 梁南凤. 基于镍基纳米材料电化学传感器的α-糖苷酶抑制剂筛选方法研究[J]. 分析化学, 2023, 51(3): 373-382. doi: 10.19756/j.issn.0253-3820.221006 shu

Citation: YANG Jian-Ping, ZHU Cai-Jun, LI Qiu-Na, CHEN Hua-Ying, HUANG Zhi-Xuan, LIU Zhi-Kang, LIANG Xiao, LIANG Nan-Feng. A Novel Method for Screening α-Glycosidase Inhibitors Based on Nickel-based Nanomaterial Electrochemical Sensor[J]. Chinese Journal of Analytical Chemistry, 2023, 51(3): 373-382. doi: 10.19756/j.issn.0253-3820.221006 shu

基于镍基纳米材料电化学传感器的α-糖苷酶抑制剂筛选方法研究

广东第二师范学院化学与材料科学学院, 广州 510303

通讯作者: 杨剑萍,E-mail:yangjianping@gdei.edu.cn

基金项目:
广州市科技计划项目(No.202002030444)、广东省教育厅重点领域专项项目(No.2022ZDZX4037)、广东省科技攀登计划项目(No.pdjh2021a0364)和大学生创新创业项目(Nos.202214278010,202214278082)资助。

摘要: 采用多周期的电化学循环伏安(CV)法在泡沫镍(NF)上一步制备了镍基纳米材料修饰电极(Ni (OH)₂/NF)用于α-糖苷酶抑制剂的酶抑制活性评价,并基于此建立了一种简便的中药糖苷酶抑制剂筛选方法。采用X射线粉末衍射仪和扫描电镜表征修饰电极表面的结构和形貌;采用CV法和计时电流法测试电极的电化学性能。结果表明,Ni (OH)₂/NF传感器检测复杂酶体系中的葡萄糖具有良好的电化学响应,灵敏度高达3222 μA·mmol/(L·cm²),线性范围为3.0~6000 μmol/L,检出限低至0.9 μmol/L (S/N=3)。采用临床降糖药物阿卡波糖验证了此传感器用于α-糖苷酶活性检测的可行性;并将传感器应用于莲须提取液的酶抑制效果评价,发现莲须具有一定的α-糖苷酶抑制活性,其半数抑制浓度(IC₅₀)为3.31 g/L。本研究结果表明,研制的传感器适用于α-糖苷酶抑制活性分析,为天然降糖药物筛选提供了一种新方法。

关键词:

English

A Novel Method for Screening α-Glycosidase Inhibitors Based on Nickel-based Nanomaterial Electrochemical Sensor

School of Chemistry and Materials Science, Guangdong University of Education, Guangzhou 510303, China

Corresponding author: YANG Jian-Ping, yangjianping@gdei.edu.cn

Received Date: 03 January 2022
Revised Date: 16 February 2023
Fund Project:
Supported by the Science and Technology Program of Guangzhou City, China (No. 202002030444), the Special Projects in Key Fields of Education Department of Guangdong Province, China (No.2022ZDZX4037), the Science and Technology Innovation Climbing Program of Guangdong Province, China (No. pdjh2021a0364) and College Students′ Innovation and Entrepreneurship Project (Nos. 202214278010, 202214278082).

Abstract: To establish a novel and simple method for screening glycosidase inhibitors from traditional Chinese medicine, a nickel-based nanomaterial modified electrode (Ni(OH)₂/NF) was prepared on nickel foam (NF) in one step by multi-cycle electrochemical cyclic voltammetry, which was used to construct a highly sensitive and selective electrochemical sensor for evaluating the inhibitory activity of α-glycosidase inhibitors. The structure and surface morphology of the modified electrode were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM), and its sensing performance was investigated by cyclic voltammetry and chronoamperometry. The results showed that the Ni(OH)₂/NF sensor had a high electrochemical response to glucose in complex enzyme reaction system, and the sensitivity was 3222 μA·mmol/(L·cm²) in the linear range of 3.0-6000 μmol/L, with a detection limit as low as 0.9 μmol/L (S/N = 3). The feasibility of the sensor for detecting α-glycosidase activity was verified by a clinical hypoglycemic drug acarbose. The nanosensor was also applied to evaluate the inhibitory effect of lotus stamen extract. It was found that lotus stamen had a certain α-glycosidase inhibitory activity, with its half inhibition concentration IC₅₀ = 3.31 g/L. The experimental results showed that the developed sensor was suitable for analysis of α-glycosidase inhibitory activity, providing a novel method for screening natural hypoglycemic chemicals.

Key words:

1. [1]
  TANG C, QIAN Z, QIAN Y, HUANG Y, ZHAO M, AO H, FENG H. Sens. Actuators, B, 2017, 245:282-289.TANG C, QIAN Z, QIAN Y, HUANG Y, ZHAO M, AO H, FENG H. Sens. Actuators, B, 2017, 245:282-289.
2. [2]
  LIU D M, DONG C, MA R T. Colloids Surf. B, 2021, 197:111400.LIU D M, DONG C, MA R T. Colloids Surf. B, 2021, 197:111400.
3. [3]
  SHI M, CEN Y, XU G, WEI F, XU X, CHENG X, CHAI Y, SOHAIL M, HU Q. Anal. Chim. Acta, 2019, 1077:225-231.SHI M, CEN Y, XU G, WEI F, XU X, CHENG X, CHAI Y, SOHAIL M, HU Q. Anal. Chim. Acta, 2019, 1077:225-231.
4. [4]
  HEDRINGTON M S, DAVIS S N. Expert Opin. Pharmacother., 2019, 20(18):2229-2235.HEDRINGTON M S, DAVIS S N. Expert Opin. Pharmacother., 2019, 20(18):2229-2235.
5. [5]
  TAN K, TESAR C, WILTON R, JEDRZEJCZAK R P, JOACHIMIAK A. Protein Sci., 2018, 27(8):1498-1508.TAN K, TESAR C, WILTON R, JEDRZEJCZAK R P, JOACHIMIAK A. Protein Sci., 2018, 27(8):1498-1508.
6. [6]
  ZHANG M, YANG R, YU S, ZHAO W. Int. J. Food Sci. Technol., 2022, 57(1):67-77.ZHANG M, YANG R, YU S, ZHAO W. Int. J. Food Sci. Technol., 2022, 57(1):67-77.
7. [7]
  XI Ling-Ling, JIAN Jing-Yi, ZHA Ding-Sheng, ZHAO Xiang-Long, WANG Jin-Cai, LI Juan, JIANG Zheng-Jin, ZHANG Ting-Ting. Acta Pharm. Sin., 2021, 56(9):2419-2425. 郗玲玲, 简敬一, 査丁胜, 赵祥龙, 汪锦才, 李娟, 江正瑾, 张婷婷. 药学学报, 2021, 56(9):2419-2425.
8. [8]
  DEVECI E, ÇAYAN F, TEL-ÇAYAN G, DURU M E. S. Afr. J. Bot., 2021, 137:19-23.DEVECI E, ÇAYAN F, TEL-ÇAYAN G, DURU M E. S. Afr. J. Bot., 2021, 137:19-23.
9. [9]
  WU X, QIU B, CHEN Y, SHI Y, ZHU J, LIU X, ZHAO D. J. Chromatogr. B, 2020, 1159:122398.WU X, QIU B, CHEN Y, SHI Y, ZHU J, LIU X, ZHAO D. J. Chromatogr. B, 2020, 1159:122398.
10. [10]
  LANDA-CANSIGNO C, HERNÁNDEZ-DOMÍNGUEZ E E, MONRIBOT-VILLANUEVA J L, LICEA-NAVARRO A F, MATEO-CID L E, SEGURA-CABRERA A, GUERRERO-ANALCO J A. Algal Res., 2020, 49:101954.LANDA-CANSIGNO C, HERNÁNDEZ-DOMÍNGUEZ E E, MONRIBOT-VILLANUEVA J L, LICEA-NAVARRO A F, MATEO-CID L E, SEGURA-CABRERA A, GUERRERO-ANALCO J A. Algal Res., 2020, 49:101954.
11. [11]
  WANG S, XIE X, ZHANG L, HU Y, WANG H, TU Z. Ind. Crop. Prod., 2020, 143:111941.WANG S, XIE X, ZHANG L, HU Y, WANG H, TU Z. Ind. Crop. Prod., 2020, 143:111941.
12. [12]
  LIU J, GUO Y, ZHANG J, QI Y, JIA X, GAO G, SHUAI J, LIU H, ZHANG B, XIAO P. Phytomedicine, 2014, 21(13):1753-1758.LIU J, GUO Y, ZHANG J, QI Y, JIA X, GAO G, SHUAI J, LIU H, ZHANG B, XIAO P. Phytomedicine, 2014, 21(13):1753-1758.
13. [13]
  PEI H, SU W, GUI M, DOU M, ZHANG Y, WANG C, LU D. Molecules, 2021, 26(7):1855.PEI H, SU W, GUI M, DOU M, ZHANG Y, WANG C, LU D. Molecules, 2021, 26(7):1855.
14. [14]
  CHEN G Y, ZHANG H, YANG F Q. Anal. Chim. Acta, 2021, 1142:19-27.CHEN G Y, ZHANG H, YANG F Q. Anal. Chim. Acta, 2021, 1142:19-27.
15. [15]
  TIAN T, CHEN G Y, ZHANG H, YANG F Q. Molecules, 2021, 26(15):4638.TIAN T, CHEN G Y, ZHANG H, YANG F Q. Molecules, 2021, 26(15):4638.
16. [16]
  WANG X L, JIAO F R, YU M, LIN L B, XIAO J, ZHANG Q, WANG L, DUAN D Z, XIE G. Bioorg. Med. Chem. Lett., 2017, 27(9):1993-1998.WANG X L, JIAO F R, YU M, LIN L B, XIAO J, ZHANG Q, WANG L, DUAN D Z, XIE G. Bioorg. Med. Chem. Lett., 2017, 27(9):1993-1998.
17. [17]
  ZHANG X, LI G, WU D, YU Y, HU N, WANG H, LI X, WU Y. Food Funct., 2020, 11(1):66-82.ZHANG X, LI G, WU D, YU Y, HU N, WANG H, LI X, WU Y. Food Funct., 2020, 11(1):66-82.
18. [18]
  CHEN Han, SUN Jian-Hang, YANG Guo-Cheng. Chin. J. Anal. Chem., 2021, 49(6):1008-1014. 陈汉, 孙健航, 杨国程. 分析化学, 2021, 49(6):1008-1014.
19. [19]
  CUI Min, WEN Xiao-Fang, REN Ju-Jie, ZHANG Cong, LI Na, HAN Yuan-Yuan, YU Xu-Dong, JI Xue-Ping. Chin. J. Anal. Chem., 2020, 48(2):224-232. 崔敏, 温晓芳, 任聚杰, 张聪, 李娜, 韩元元, 余旭东, 籍雪平. 分析化学, 2020, 48(2):224-232.
20. [20]
  XIE W, YANG G, XU M, BO X. New J. Chem., 2020, 42(6):3963.XIE W, YANG G, XU M, BO X. New J. Chem., 2020, 42(6):3963.
21. [21]
  DONG Q, RYU H, LEI Y. Electrochim. Acta, 2021, 370:137744.DONG Q, RYU H, LEI Y. Electrochim. Acta, 2021, 370:137744.
22. [22]
  REN Z, MAO H, LUO H, DENG X, LIU Y. Nanotechnology, 2020, 31(18):185501.REN Z, MAO H, LUO H, DENG X, LIU Y. Nanotechnology, 2020, 31(18):185501.
23. [23]
  CHANDRASEKARAN N I, HARSHINY M, THANGASAMY P, MATHESWARAN M. Appl. Phys. A, 2021, 127(1):20.CHANDRASEKARAN N I, HARSHINY M, THANGASAMY P, MATHESWARAN M. Appl. Phys. A, 2021, 127(1):20.
24. [24]
  GUO S, ZHANG C, YANG M, ZHOU Y, BI C, LV Q, MA N. Anal. Chim. Acta, 2020, 1109:130-139.GUO S, ZHANG C, YANG M, ZHOU Y, BI C, LV Q, MA N. Anal. Chim. Acta, 2020, 1109:130-139.
25. [25]
  ELAKKIYA R, MATHANKUMAR S, MADURAIVEERAN G. Mater. Chem. Phys., 2021, 269:124770.ELAKKIYA R, MATHANKUMAR S, MADURAIVEERAN G. Mater. Chem. Phys., 2021, 269:124770.
26. [26]
  ARIVAZHAGAN M, MANOVA SANTHOSH Y, MADURAIVEERAN G. Micromachines, 2021, 12(4):403.ARIVAZHAGAN M, MANOVA SANTHOSH Y, MADURAIVEERAN G. Micromachines, 2021, 12(4):403.
27. [27]
  ZHANG Y, ZHENG D, LIU S, QIN S, SUN X, WANG Z, QIN C, LI Y, ZHOU J. Appl. Surf. Sci., 2021, 552:149529.ZHANG Y, ZHENG D, LIU S, QIN S, SUN X, WANG Z, QIN C, LI Y, ZHOU J. Appl. Surf. Sci., 2021, 552:149529.
28. [28]
  GAO X, FENG W, ZHU Z, WU Z, LI S, KAN S, QIU X, GUO A, CHEN W, YIN K. Adv. Mater. Interfaces, 2021, 8(7):2002133.GAO X, FENG W, ZHU Z, WU Z, LI S, KAN S, QIU X, GUO A, CHEN W, YIN K. Adv. Mater. Interfaces, 2021, 8(7):2002133.
29. [29]
  MARINI S, BEN MANSOUR N, HJIRI M, DHAHRI R, EL MIR L, ESPRO C, BONAVITA A, GALVAGNO S, NERI G, LEONARDI S G. Electroanalysis, 2018, 30(4):727-733.MARINI S, BEN MANSOUR N, HJIRI M, DHAHRI R, EL MIR L, ESPRO C, BONAVITA A, GALVAGNO S, NERI G, LEONARDI S G. Electroanalysis, 2018, 30(4):727-733.
30. [30]
  SUN S, SHI N, LIAO X, ZHANG B, YIN G, HUANG Z, CHEN X, PU X. Appl. Surf. Sci., 2020, 529:147067.SUN S, SHI N, LIAO X, ZHANG B, YIN G, HUANG Z, CHEN X, PU X. Appl. Surf. Sci., 2020, 529:147067.
31. [31]
  ZHANG J, GONG W, YIN H, WANG D, ZHANG Y, ZHANG H, WANG G, ZHAO H. ChemSusChem, 2021, 14(14):2935-2942.ZHANG J, GONG W, YIN H, WANG D, ZHANG Y, ZHANG H, WANG G, ZHAO H. ChemSusChem, 2021, 14(14):2935-2942.
32. [32]
  YUAN G, WANG L, ZHANG X, WANG Q. J. Colloid Interface Sci., 2019, 536:189-195.YUAN G, WANG L, ZHANG X, WANG Q. J. Colloid Interface Sci., 2019, 536:189-195.
33. [33]
  KUNG C W, CHENG Y H, HO K C. Sens. Actuators, B, 2014, 204:159-166.KUNG C W, CHENG Y H, HO K C. Sens. Actuators, B, 2014, 204:159-166.
34. [34]
  ZHAO Y, GU G, YOU S, JI R, SUO H, ZHAO C, LIU F. RSC Adv., 2015, 5(66):53665-53670.ZHAO Y, GU G, YOU S, JI R, SUO H, ZHAO C, LIU F. RSC Adv., 2015, 5(66):53665-53670.
35. [35]
  LI G, WANG X, LIU L, LIU R, SHEN F, CUI Z, CHEN W, ZHANG T. Small, 2015, 11(6):731-739.LI G, WANG X, LIU L, LIU R, SHEN F, CUI Z, CHEN W, ZHANG T. Small, 2015, 11(6):731-739.
36. [36]
  LIU D, CHEN J, SHI Y. Talanta, 2017, 164:548-555.LIU D, CHEN J, SHI Y. Talanta, 2017, 164:548-555.
37. [37]
  LI Hua-Ling, WANG Xin, LIU You-Ping, DI Xin. J. Shenyang Pharm. Univ., 2015, 32(12):975-979. 李化玲, 王鑫, 刘有平, 邸欣. 沈阳药科大学学报, 2015, 32(12):975-979.
38. [38]
  LIU Y, LI H, LU L, SUN B, HUANG L, CHEN H, QIU W, TAO J, ZHAO P. J. Electrochem. Soc., 2019, 166(2):B133-B140.LIU Y, LI H, LU L, SUN B, HUANG L, CHEN H, QIU W, TAO J, ZHAO P. J. Electrochem. Soc., 2019, 166(2):B133-B140.

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沈阳化工大学材料科学与工程学院沈阳 110142

基于镍基纳米材料电化学传感器的α-糖苷酶抑制剂筛选方法研究

通讯作者: 杨剑萍,E-mail:yangjianping@gdei.edu.cn

English

A Novel Method for Screening α-Glycosidase Inhibitors Based on Nickel-based Nanomaterial Electrochemical Sensor

Corresponding author: YANG Jian-Ping, yangjianping@gdei.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于镍基纳米材料电化学传感器的α-糖苷酶抑制剂筛选方法研究

通讯作者: 杨剑萍,E-mail:yangjianping@gdei.edu.cn

English

A Novel Method for Screening α-Glycosidase Inhibitors Based on Nickel-based Nanomaterial Electrochemical Sensor

Corresponding author: YANG Jian-Ping, yangjianping@gdei.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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