引用本文:
Min ZHANG, Li Ming DU. Simultaneous Determination of Magnolol and Honokiol by Synchronous Fluorescence Spectroscopy[J]. Chinese Chemical Letters,
2006, 17(12): 1603-1606.
Citation: Min ZHANG, Li Ming DU. Simultaneous Determination of Magnolol and Honokiol by Synchronous Fluorescence Spectroscopy[J]. Chinese Chemical Letters, 2006, 17(12): 1603-1606.
Citation: Min ZHANG, Li Ming DU. Simultaneous Determination of Magnolol and Honokiol by Synchronous Fluorescence Spectroscopy[J]. Chinese Chemical Letters, 2006, 17(12): 1603-1606.
Simultaneous Determination of Magnolol and Honokiol by Synchronous Fluorescence Spectroscopy
摘要:
A simple sensitive and quick assay for simultaneously determining magnolol (MOL)and honokiol (HOL) has been described based on their natural fluorescence. This method is based on the fact that synchronous fluorometry could resolve the overlapping of fluorescence spectra, which was aroused by their similar molecular structures. In this work, the synchronous spectrum, maintaining a constant difference of Δλ=10 nm between the emission and excitation wavelengths, has been selected for the determination of HOL and MOL. Under the optimum conditions, the fluorescence intensity is proportional to the concentration of MOL and HOL in solution over the range 0.075-0.7 μg/mL and 0.05-0.9 μg/mL with the detection limit of 0.029 μg/mL and 0.019 μg/mL, respectively. The method was applied to the simultaneous determination of MOL and HOL in pharmaceutical dosage with satisfactory results.
-
关键词:
- Magnolol
- / honokiol
- / synchronous fluorometry
English
Simultaneous Determination of Magnolol and Honokiol by Synchronous Fluorescence Spectroscopy
Abstract:
A simple sensitive and quick assay for simultaneously determining magnolol (MOL)and honokiol (HOL) has been described based on their natural fluorescence. This method is based on the fact that synchronous fluorometry could resolve the overlapping of fluorescence spectra, which was aroused by their similar molecular structures. In this work, the synchronous spectrum, maintaining a constant difference of Δλ=10 nm between the emission and excitation wavelengths, has been selected for the determination of HOL and MOL. Under the optimum conditions, the fluorescence intensity is proportional to the concentration of MOL and HOL in solution over the range 0.075-0.7 μg/mL and 0.05-0.9 μg/mL with the detection limit of 0.029 μg/mL and 0.019 μg/mL, respectively. The method was applied to the simultaneous determination of MOL and HOL in pharmaceutical dosage with satisfactory results.
-
Key words:
- Magnolol
- / honokiol
- / synchronous fluorometry
扫一扫看文章
计量
- PDF下载量: 3
- 文章访问数: 740
- HTML全文浏览量: 5
下载:
