引用本文:
Shui Sheng HONG, Jia CHEN, Jian Guo ZHANG, Yan Chun TAO, Lan Ying LIU. Purification and Structure Identification of Hyaluronic Acid[J]. Chinese Chemical Letters,
2004, 15(7): 811-812.
Citation: Shui Sheng HONG, Jia CHEN, Jian Guo ZHANG, Yan Chun TAO, Lan Ying LIU. Purification and Structure Identification of Hyaluronic Acid[J]. Chinese Chemical Letters, 2004, 15(7): 811-812.
Citation: Shui Sheng HONG, Jia CHEN, Jian Guo ZHANG, Yan Chun TAO, Lan Ying LIU. Purification and Structure Identification of Hyaluronic Acid[J]. Chinese Chemical Letters, 2004, 15(7): 811-812.
Purification and Structure Identification of Hyaluronic Acid
摘要:
Polysaccharide produced by mutated strain of Streptococcus zooepidemicus was purified by the procedures including Savage method, quaternary ammonium compound precipitation, DEAE-cellulose(DE52) chromatography and Sephadex G-75 gel filtration. The structure of the purified polysaccharide has been characterized by means of chemical composition analysis, 13C NMR spectrum, infrared spectrum and circular dichroism (CD). All the results showed that the purified polysaccharide was hyaluronic acid (HA). The single helix conformation of the purified HA was determined by Congo red experiment. The molecular weight of the HA was about 1.16×106D, which was measured by viscosity method.
English
Purification and Structure Identification of Hyaluronic Acid
Abstract:
Polysaccharide produced by mutated strain of Streptococcus zooepidemicus was purified by the procedures including Savage method, quaternary ammonium compound precipitation, DEAE-cellulose(DE52) chromatography and Sephadex G-75 gel filtration. The structure of the purified polysaccharide has been characterized by means of chemical composition analysis, 13C NMR spectrum, infrared spectrum and circular dichroism (CD). All the results showed that the purified polysaccharide was hyaluronic acid (HA). The single helix conformation of the purified HA was determined by Congo red experiment. The molecular weight of the HA was about 1.16×106D, which was measured by viscosity method.
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Key words:
- Streptococcus zooepidemicus
- / hyaluronic acid
- / structure identification
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