间接顶空固相微萃取-气相色谱-质谱法对红花香雪兰天然花香成分的分析

引用本文: 刘宝峰, 高丰展, 房强, 王丽. 间接顶空固相微萃取-气相色谱-质谱法对红花香雪兰天然花香成分的分析[J]. 分析化学, 2016, 44(3): 444-450. doi: 10.11895/j.issn.0253-3820.150831 shu

Citation: LIU Bao-Feng, GAO Feng-Zhang, FANG Qiang, WANG Li. Determination of Red Freesia Flower Volatiles with Indirect Headspace Solid Phase Microextraction Coupled to Gas Chromatography and Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2016, 44(3): 444-450. doi: 10.11895/j.issn.0253-3820.150831 shu

间接顶空固相微萃取-气相色谱-质谱法对红花香雪兰天然花香成分的分析

1.
东北师范大学生命科学学院, 长春 130024

通讯作者: 王丽

基金项目:
本文系国家自然科学基金(Nos.31170276,31300271)资助项目 (Nos.31170276,31300271)

摘要: 建立了间接顶空固相微萃取-气相色谱-质谱对红花香雪兰天然花香成分的分析方法。首先利用气体采样装置对花香成分进行固相富集,富集后的花香成分在浸提液中解析,之后利用顶空固相微萃取方法对浸提液中的花香成分进行分析。红花香雪兰花香中主要成分(沉香醇、松油醇、紫罗兰酮和二氢紫罗兰酮)定量分析优化实验条件:Florisil(弗罗里硅土)作为吸附剂,水-甲醇混合液(9:1, V/V)和0.6 mol/L HCl作为浸提液,萃取温度70℃,平衡时间为25 min。实验结果表明,沉香醇和松油醇在1-100 μg/mL范围内有良好的线性关系(R²≥0.981),检出限分别为0.05和0.10 μg; 二氢紫罗兰酮和紫罗兰酮在0.5-50 μg/mL范围内有良好的线性关系(R²≥0.988),检出限为0.02 μg。

关键词:

English

Determination of Red Freesia Flower Volatiles with Indirect Headspace Solid Phase Microextraction Coupled to Gas Chromatography and Mass Spectrometry

1.
Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China

Corresponding author: 王丽

Received Date: 20 October 2015
Fund Project:
本文系国家自然科学基金(Nos.31170276,31300271)资助项目

Abstract: A method was established for the analysis of red Freesia flower volatiles by indirect headspace solid phase microextraction (HS-SPME). The gas collecting tube was used to enrich flower volatiles, and adsorbing material was resolved in the extraction solution. Then, the adsorbing material was analyzed by HS-SPME. The experimental conditions for the quantification of major flower volatiles (linalool, terpineol, ionone and dihydrogen-ionone) were optimized. Florisil was used as adsorbent, with water-methanol solution (9:1, V/V) and 0.6 mol/L hydrochloric acid as the extraction solution. The thermal desorption temperature was 70℃ and the extraction time was 25 min. The results showed that the concentration range of linear relationship for linalool and terpineol (R²≥0.981) was 1-100 μg/mL, with detection limits of 0.05 and 0.10 μg, respectively, and 0.5-50 μg/mL for dihydro ionone and ionone (R²≥0.988) with a detection limit of 0.02 μg.

Key words:

Red Freesia
/ Flower volatiles
/ Indirect headspace solid phase microextraction
/ Gas chromatography-mass spectrometry

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

间接顶空固相微萃取-气相色谱-质谱法对红花香雪兰天然花香成分的分析

通讯作者: 王丽

English

Determination of Red Freesia Flower Volatiles with Indirect Headspace Solid Phase Microextraction Coupled to Gas Chromatography and Mass Spectrometry

Corresponding author: 王丽

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

间接顶空固相微萃取-气相色谱-质谱法对红花香雪兰天然花香成分的分析

通讯作者: 王丽

English

Determination of Red Freesia Flower Volatiles with Indirect Headspace Solid Phase Microextraction Coupled to Gas Chromatography and Mass Spectrometry

Corresponding author: 王丽

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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