核酸适配体介导的荧光铜纳米簇用于免标记快速检测水胺硫磷

肖淑芬 方政 王旭军 杨添惠 卿太平

引用本文: 肖淑芬, 方政, 王旭军, 杨添惠, 卿太平. 核酸适配体介导的荧光铜纳米簇用于免标记快速检测水胺硫磷[J]. 分析化学, 2023, 51(3): 364-372. doi: 10.19756/j.issn.0253-3820.221207 shu
Citation:  XIAO Shu-Fen,  FANG Zheng,  WANG Xu-Jun,  YANG Tian-Hui,  QING Tai-Ping. Aptamer-Mediated Fluorescent Copper Nanoclusters for Label-free and Rapid Detection of Isocarbophos[J]. Chinese Journal of Analytical Chemistry, 2023, 51(3): 364-372. doi: 10.19756/j.issn.0253-3820.221207 shu

核酸适配体介导的荧光铜纳米簇用于免标记快速检测水胺硫磷

    通讯作者: 卿太平,E-mail:taiping_qing@163.com
  • 基金项目:

    湖南省自然科学基金项目(No.2022JJ30559)、化学生物传感与计量学国家重点实验室开放基金项目(No.2019008)和湘潭大学大学生创新创业训练项目资助。

摘要: 基于核酸适配体的靶标识别能力和铜纳米簇(CuNCs)优良的荧光性能,本研究开发了一种免标记荧光探针用于有机磷农药水胺硫磷(ISO)的快速检测。当靶标分子ISO不存在时,溶液中ISO的核酸适配体和互补DNA形成双链结构,从而介导合成荧光CuNCs,表现出高的荧光信号;当待测样品中存在ISO时,ISO与其核酸适配体形成复合物,释放出单链互补DNA。游离的单链DNA不能介导合成CuNCs,导致溶液中的荧光信号减弱。在最佳条件下,检测体系的荧光抑制率与ISO浓度的对数在0.05~25 mg/L浓度范围内呈线性关系,检出限为47 μg/L (3σ),其它物质对其检测几乎没有干扰。将此探针用于检测水样中的ISO,回收率为80.3%~108.0%。研究结果表明,本方法可用于检测实际样品中的ISO残留。

English


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  • 收稿日期:  2022-04-27
  • 修回日期:  2022-12-25
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