桑色素分子印迹传感器的制备与应用

引用本文: 刘蓉, 龙立平, 雷存喜, 吴朝阳, 刘煜, 李思佳. 桑色素分子印迹传感器的制备与应用[J]. 分析化学, 2016, 44(3): 385-390. doi: 10.11895/j.issn.0253-3820.150761 shu

Citation: LIU Rong, LONG Li-Ping, LEI Cun-Xi, Wu Zhao-Yang, LIU Yu, LI Si-Jia. Fabrication and Application of a Molecularly Imprinted Sensor for Morin Detection[J]. Chinese Journal of Analytical Chemistry, 2016, 44(3): 385-390. doi: 10.11895/j.issn.0253-3820.150761 shu

桑色素分子印迹传感器的制备与应用

刘蓉 ^1,2, ,
龙立平 ^{1,
,} ,
雷存喜 ^1, ,
吴朝阳 ^2, ,
刘煜 ^1, ,
李思佳 ^1,

1.
¹ 湖南城市学院化学与环境工程学院, 益阳 413000;
2.
² 湖南大学化学生物传感与计量学国家重点实验室, 长沙 410082

通讯作者: 龙立平

基金项目:
本文系湖南省教育厅高校科研一般项目(No.15C0244) (No.15C0244)

国家自然科学基金项目(No.21545003) (No.21545003)

湖南省自然科学基金项目(No.2015JJ2023) (No.2015JJ2023)

湖南省高校科技创新团队支持计划项目(No.2014)207号)资助 (No.2014)207号)

摘要: 以邻氨基酚(o-AP)为功能单体,桑色素为模板分子,基于分子间的相互作用力,在金电极表面电聚合制备具有特异性识别孔穴的桑色素分子印迹传感器膜。采用循环伏安法(CV)、差分脉冲伏安法(DPV)等研究了分子印迹膜的性能和分子印迹效应。探索了聚合膜配比及聚合扫描圈数对传感器性能的影响,优化了洗脱时间和印迹时间。比较了此传感器对其结构相似物的选择性响应,发现其对桑色素检测具有良好的选择性。在最佳实验条件下,此传感器对桑色素浓度定量测定范围为0.05~1.70 μmol/L,线性方程为I(μA)=1.0800lgc(mol/L)+ 9.3599, R=0.9934,检出限为0.01 μmol/L。用此传感器测定黑茶样品中桑色素的含量,加标回收率为104.0%-108.0%。

关键词:

English

Fabrication and Application of a Molecularly Imprinted Sensor for Morin Detection

1.
¹ College of Chemistry & Environmental Engineering, Hunan City University, Yiyang 413000, China;
2.
² State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China

Corresponding author: 龙立平

Received Date: 26 September 2015
Fund Project:
本文系湖南省教育厅高校科研一般项目(No.15C0244)

国家自然科学基金项目(No.21545003)

湖南省自然科学基金项目(No.2015JJ2023)

湖南省高校科技创新团队支持计划项目(No.2014)207号)资助

Abstract: Based on the intermolecular interaction, a molecularly imprinted polymer electrochemical sensor with specific identification of cavity on gold electrode surface was proposed with o-aminophenol as functional monomers and morin as template molecule. The performance and effect of MIP were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Factors affecting the properties of sensor, such as polymeric membrane ratio, scanning cycles, elution time and adsorption time were investigated. Substances which had a similar structure to morin was used to compared the selective response. The result showed that the sensor had a good selectivity to morin. Under the optimized experiment conditions, the current response of the imprinted sensor was linear to the concentration of morin in the range of 0.05-1.7 μmol/L with linear equation as follows: I(μA)= 1.0800lgc(mol/L)+ 9.3599(R=0.9934), and the detection limit of 0.1 μmol/L. The sensor was used to determine the content of morin in black tea samples, and the recoveries of standard addition were between 104.0% and 108.0%.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

桑色素分子印迹传感器的制备与应用

通讯作者: 龙立平

English

Fabrication and Application of a Molecularly Imprinted Sensor for Morin Detection

Corresponding author: 龙立平

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

桑色素分子印迹传感器的制备与应用

通讯作者: 龙立平

English

Fabrication and Application of a Molecularly Imprinted Sensor for Morin Detection

Corresponding author: 龙立平

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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