人乳寡糖的高分辨质谱结构分析及液相色谱条件的比较

引用本文: 王海燕, 张曦美, 崔秀秀, 康鹏, 张艳, 张佳莹, 葛武鹏. 人乳寡糖的高分辨质谱结构分析及液相色谱条件的比较[J]. 分析化学, 2022, 50(2): 278-289. doi: 10.19756/j.issn.0253-3820.210603 shu

Citation: WANG Hai-Yan, ZHANG Xi-Mei, CUI Xiu-Xiu, KANG Peng, ZHANG Yan, ZHANG Jia-Ying, GE Wu-Peng. Structural Analysis of Human Milk Oligosaccharides via High Resolution Mass Spectrometry and Optimization of Chromatographic Separation Conditions[J]. Chinese Journal of Analytical Chemistry, 2022, 50(2): 278-289. doi: 10.19756/j.issn.0253-3820.210603 shu

人乳寡糖的高分辨质谱结构分析及液相色谱条件的比较

王海燕 ^1, ,
张曦美 ^1, ,
崔秀秀 ^2, ,
康鹏 ^1, ,
张艳 ^3, ,
张佳莹 ^1, ,
葛武鹏 ^{1,
,}

1.
西北农林科技大学食品科学与工程学院,杨凌 712100;
2.
西安百跃羊乳集团有限公司,西安 710000;
3.
陕西省羊乳产品质量监督检验中心,富平 711700

通讯作者: 葛武鹏,E-mail:josephge@nwafu.edu.cn

基金项目:
校企合作项目（No.K4030220076）资助

摘要: 人乳寡糖(Human milk oligosaccharides，HMOs)与婴幼儿生命初期的生长发育密切相关，揭示其组成及结构有助于阐明HMOs功能。本研究通过超高效液相色谱-高分辨质谱联用技术，对19个HMOs(16个中性HMOs、3个酸性HMOs)进行了结构解析，在寡糖异构体裂解规律的基础上详细探讨了4组异构体的特征碎片离子，比较了3种超高效液相色谱条件下HMOs异构体分离效果的差异，为复杂HMOs异构体的结构解析及高效液相色谱条件的选择提供了科学依据。

关键词:

English

Structural Analysis of Human Milk Oligosaccharides via High Resolution Mass Spectrometry and Optimization of Chromatographic Separation Conditions

1.
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;
2.
Xi'an Baiyue Goat Group Co. Ltd., Xi'an 710000, China;
3.
Shaanxi Goat Milk Product Quality Supervision and Inspection Center, Fuping 711700, China

Corresponding author: GE Wu-Peng, josephge@nwafu.edu.cn

Received Date: 01 July 2021
Revised Date: 25 November 2021
Fund Project:
Supported by the School-Enterprise Cooperation Project (No.K4030220076)

Abstract: Human milk oligosaccharides (HMOs) are closely related to the growth and development of infants in early life. In this study, the structures of 19 HMOs (16 neutral HMOs and 3 sialylated HMOs) were analyzed by ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Based on the cleavage rules of oligosaccharide isomers, the differences of fragment ions among the four groups of isomers were discussed. The separation effects of HMOs isomers under three different UHPLC conditions were compared, which provided scientific basis for the structural analysis of complex HMOs isomers and the selection of UHPLC conditions.

Key words:

Human milk oligosaccharides
/ Ultra-high performance liquid chromatography-high resolution mass spectrometry
/ Elution condition
/ Isomers
/ Structural analysis

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

人乳寡糖的高分辨质谱结构分析及液相色谱条件的比较

通讯作者: 葛武鹏,E-mail:josephge@nwafu.edu.cn

English

Structural Analysis of Human Milk Oligosaccharides via High Resolution Mass Spectrometry and Optimization of Chromatographic Separation Conditions

Corresponding author: GE Wu-Peng, josephge@nwafu.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

人乳寡糖的高分辨质谱结构分析及液相色谱条件的比较

通讯作者: 葛武鹏,E-mail:josephge@nwafu.edu.cn

English

Structural Analysis of Human Milk Oligosaccharides via High Resolution Mass Spectrometry and Optimization of Chromatographic Separation Conditions

Corresponding author: GE Wu-Peng, josephge@nwafu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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