青霉素酶-氧化苏木精修饰Au/ZnO/石墨烯基青霉素电化学传感器的研制

引用本文: 韩志钟, 吴月婷, 周莹, 潘海波, 陈敬华, 李春艳. 青霉素酶-氧化苏木精修饰Au/ZnO/石墨烯基青霉素电化学传感器的研制[J]. 分析化学, 2016, 44(3): 377-384. doi: 10.11895/j.issn.0253-3820.150694 shu

Citation: HAN Zhi-Zhong, WU Yue-Ting, ZHOU Ying, PAN Hai-Bo, CHEN Jing-Hua, LI Chun-Yan. A Low Detection Limit Penicillin Electrochemical Biosensor Based on Penicillinase-Hematein Au/ZnO/Single Graphene Nanosheets[J]. Chinese Journal of Analytical Chemistry, 2016, 44(3): 377-384. doi: 10.11895/j.issn.0253-3820.150694 shu

青霉素酶-氧化苏木精修饰Au/ZnO/石墨烯基青霉素电化学传感器的研制

韩志钟 ^1, ,
吴月婷 ^2,3, ,
周莹 ^1, ,
潘海波 ^{2,
,} ,
陈敬华 ^1, ,
李春艳 ^1,

1.
¹ 福建医科大学药学院, 福州 350108;
2.
² 福州大学化学学院, 福州 350108;
3.
³ 福建汇顺职业健康评价有限公司, 泉州 362000

通讯作者: 潘海波

基金项目:
本文系国家自然科学基金(No.21375017) (No.21375017)

福建省自然科学基金(No.2015J05020) (No.2015J05020)

福建省卫生计生委青年科研课题(No.2014-1-39) (No.2014-1-39)

福建医科大学苗圃科研基金(No.2014MP008)资助 (No.2014MP008)

摘要: 采用均匀沉淀法合成ZnO纳米颗粒(ZnO NPs),以ZnO NPs为种子,制备水溶性Au/ZnO异质结构。将Au/ZnO异质结构附着于离子液体功能化石墨烯(GN)复合膜上,形成一种新颖的负载型石墨烯复合材料(Au/ZnO/GN)。所构建的青霉素酶-氧化苏木精修饰Au/ZnO/GN(PH-AZG)传感器在PBS水溶液(pH=7.0)中对青霉素钠检测线性范围为2.5×10^-14~3.3×10^-6 mol/L,检出限达到1.5×10^-14 mol/L (S/N≥ 3)。在相同条件下制备5根PH-AZG电极,其响应电流的相对标准偏差(RSD)小于3.2%。同时,在实际牛奶制品中,本方法的检测线性范围为5×10^-14~5×10^-7 mol/L,加标回收率为 99.7%-101.4%,RSD为 2.3%-3.5% (n=5)。结果表明,本方法对实际牛奶制品中低浓度青霉素钠的检测具有可行性。

关键词:

English

A Low Detection Limit Penicillin Electrochemical Biosensor Based on Penicillinase-Hematein Au/ZnO/Single Graphene Nanosheets

1.
¹ School of Pharmacy, Fujian Medical University, Fuzhou 350108, China;
2.
² College of Chemistry, Fuzhou University, Fuzhou 350108, China;
3.
³ Fujian Huishun Professional Health Evaluation Co. Ltd., Quanzhou 362000, China

Corresponding author: 潘海波

Received Date: 31 August 2015
Fund Project:
本文系国家自然科学基金(No.21375017)

福建省自然科学基金(No.2015J05020)

福建省卫生计生委青年科研课题(No.2014-1-39)

福建医科大学苗圃科研基金(No.2014MP008)资助

Abstract: ZnO nanoparticles (ZnO NPs) were obtained by a direct precipitation method. With the as-prepared ZnO NPs as seeds, Au/ZnO heterostructure was synthesized by seed-mediated growth method without any surfactant, and the diameters of ZnO NPs and Au NPs were about 50 nm and 10 nm, respectively. Then ionic liquids (ILs), trihexyltetradecylphosphonium bis (trifluoromethylsulfonyl) imide ([P(C₆)₃C₁₄][Tf₂N]), and functionalized graphene (GN) were prepared under room temperature. The ILs as bridges could connect Au/ZnO heterostructure to form a new kind of graphene nanocomposite, Au/ZnO/GN. Then the penicillinase and hematein were immobilized on Au/ZnO/GN. And the biosensors based on penicillinase-hematein-Au/ZnO/GN (PH-AZG) were used for detecting penicillin G. In PBS buffer solution (pH 7.0), PH-AZG exhibited a detection range from 2.5×10^-14 to 3.3×10^-6 mol/L with a detection limit of 1.5×10^-14 mol/L (S/N≥ 3). Five PH-AZG electrodes were prepared with the same conditions, and the RSDs for their current response were less than 3.2%. Furthermore, the standard curves were linear in the range of 5×10^-14-5×10^-7 mol/L for milk. The average recoveries were 99.7%-101.4% with RSDs of 2.3%-3.5% (n=5). The method is sensitive and repeatable, and can be applied to the field of residue analysis about penicillins G with low concentration levels.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

青霉素酶-氧化苏木精修饰Au/ZnO/石墨烯基青霉素电化学传感器的研制

通讯作者: 潘海波

English

A Low Detection Limit Penicillin Electrochemical Biosensor Based on Penicillinase-Hematein Au/ZnO/Single Graphene Nanosheets

Corresponding author: 潘海波

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

青霉素酶-氧化苏木精修饰Au/ZnO/石墨烯基青霉素电化学传感器的研制

通讯作者: 潘海波

English

A Low Detection Limit Penicillin Electrochemical Biosensor Based on Penicillinase-Hematein Au/ZnO/Single Graphene Nanosheets

Corresponding author: 潘海波

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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