梅花鹿茸快速生长及骨化期相关差异表达蛋白分析

幺宝金 张梅 刘美辰 王群 刘宇昕 赵雨

引用本文: 幺宝金,  张梅,  刘美辰,  王群,  刘宇昕,  赵雨. 梅花鹿茸快速生长及骨化期相关差异表达蛋白分析[J]. 分析化学, 2018, 46(7): 1152-1159. doi: 10.11895/j.issn.0253-3820.171394 shu
Citation:  YAO Bao-Jin,  ZHANG Mei,  LIU Mei-Chen,  WANG Qun,  LIU Yu-Xin,  ZHAO Yu. Analysis of Differential Proteins of Sika Deer Antler at Rapid Growth and Ossification Stages[J]. Chinese Journal of Analytical Chemistry, 2018, 46(7): 1152-1159. doi: 10.11895/j.issn.0253-3820.171394 shu

梅花鹿茸快速生长及骨化期相关差异表达蛋白分析

  • 基金项目:

    本文系吉林省教育厅"十三五"科学技术研究项目(No.JJKH20170721KJ)和吉林省科技厅优秀青年人才基金项目(No.20170520044JH)资助

摘要: 采用同位素标记相对定量(iTRAQ)技术、超高效液相色谱-质谱联用技术和生物信息学分析方法对东北梅花鹿快速生长期(60 d)与骨化期鹿茸(90 d)进行比较蛋白质组学研究,共鉴定出127种差异蛋白。与骨化期鹿茸比较,快速生长期鹿茸中显著上调的差异蛋白80种,显著下调差异蛋白47种,这些差异表达蛋白主要分布在细胞外基质、核小体、珠蛋白-血红蛋白复合物、肌动蛋白丝、内质网-高尔基中隔室、内质网官腔及核内膜等部位。快速生长期鹿茸显著上调的差异蛋白主要是参与血氧运输、神经生长和再生、软骨和骨组织发育及ATP合成的功能蛋白,而显著下调的差异蛋白主要为参与软骨内骨化过程的功能蛋白,这些蛋白在鹿茸不同生长时期表达量的变化与鹿茸的快速生长和骨化进程密切相关。本研究为系统揭示鹿茸快速生长及骨化的分子机制提供了基础数据,同时对研究鹿茸的药效物质基础及临床应用具有重要的指导意义。

English

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  • 收稿日期:  2017-11-06
  • 修回日期:  2018-04-28
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