直接获取生物组织内部磷脂类物质的质谱分析方法

引用本文: 卢海艳, 张建勇, 周炜, 魏益平, 陈焕文. 直接获取生物组织内部磷脂类物质的质谱分析方法[J]. 分析化学, 2016, 44(3): 329-334. doi: 10.11895/j.issn.0253-3820.160034 shu

Citation: LU Hai-Yan, ZHANG Jian-Yong, ZHOU Wei, WEI Yi-Ping, CHEN Huan-Wen. Direct Analysis of Phospholipids in Biological Tissues Using Internal Exlractive Electrospray Ionization Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2016, 44(3): 329-334. doi: 10.11895/j.issn.0253-3820.160034 shu

直接获取生物组织内部磷脂类物质的质谱分析方法

卢海艳 ^1, ,
张建勇 ^2, ,
周炜 ^1, ,
魏益平 ^2, ,
陈焕文 ^{1,
,}

1.
¹ 江西省质谱科学与仪器重点实验室, 东华理工大学, 南昌 330013;
2.
² 南昌大学第二附属医院, 南昌 330006

通讯作者: 陈焕文

基金项目:
本文系国家自然科学基金(Nos.21565004,21225522) (Nos.21565004,21225522)

长江学者和创新团队发展计划项目(No.IRT13054) (No.IRT13054)

摘要: 选择肺癌组织等多种生物组织为代表性样品, 在正离子检测模式下, 以磷脂类物质丰度、信号强度高为目标, 考察了21种不同组成的CH₃OH/H₂O溶剂体系, 获得了最佳溶剂(CH₃OH-H₂O, 30:70, V/V), 建立了一种无需破坏、研磨即可获取组织内部样品中磷脂类物质信号的内部萃取电喷雾电离质谱(iEESI-MS)方法, 并成功应用于肺癌、食管癌、猪肉、牛肉、猪肺、猪心等不同生物组织样品中磷脂类物质的直接质谱分析。本方法无需样品预处理, 可通过选择合适的萃取溶剂来提高方法的分析灵敏度和选择性, 单个样品的平均分析时间少于1 min, 样品耗量少, 有望为生物组织样品中磷脂类物质的研究提供一种质谱学新方法。

关键词:

English

Direct Analysis of Phospholipids in Biological Tissues Using Internal Exlractive Electrospray Ionization Mass Spectrometry

1.
¹ East China Institute of Technology, Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, Nanchang 330013, China;
2.
² Second Affiliated Hospital of Nanchang University, Nanchang 330006, China

Corresponding author: 陈焕文

Received Date: 14 January 2016
Fund Project:
本文系国家自然科学基金(Nos.21565004,21225522)

长江学者和创新团队发展计划项目(No.IRT13054)

Abstract: Phospholipids and their metabolites play an important role in a variety of cellular processes including cell-cell adhesion, cell growth and differentiation, apoptosis, phagocytosis as well as storage of energy. In this study, the phospholipid composition of cancer tissue and adjacent normal tissue from humans and animals were analyzed by internal extractive electrospray ionization mass spectrometry (iEESI-MS). Extractive solvent at high voltage (+5.5 kV) was injected into tissue samples using a fused silica capillary at a flow rate of 0.5-1 μL/min, producing fine charged droplets containing analytes of tissue samples at the tip of the sample. Charged droplets were directly sampled to the atmospheric inlet of a mass spectrometer. Out of 21 different ratios of CH₃OH:H₂O solvent mixture, the ratio CH₃OH:H₂O=30:70 (V/V) showed the optimal phospholipids extraction and visibility in MS. A large number of phospholipids from different tissue samples (such as cancer tissue and adjacent normal tissue of lung cancer, esophageal cancer tissue, pork, beef, porcine heart and porcine lung) were obtained simultaneously by iEESI-MS analysis. The experimental results demonstrated that iEESI-MS was characterized by minimal sample pretreatment, low sample consumption, and rapid analysis (the analysis time per sample was less than 1 min), and the selectivity and sensitivity of iEESI-MS could be improved by choosing proper solvent. Importantly, the experimental results provided new information for further studies of phospholipids in biological tissues.

Key words:

Biological tissue
/ Phospholipids
/ Extractive solvent
/ Internal extractive electrospary inozation mass spectrometry

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沈阳化工大学材料科学与工程学院沈阳 110142

直接获取生物组织内部磷脂类物质的质谱分析方法

通讯作者: 陈焕文

English

Direct Analysis of Phospholipids in Biological Tissues Using Internal Exlractive Electrospray Ionization Mass Spectrometry

Corresponding author: 陈焕文

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

直接获取生物组织内部磷脂类物质的质谱分析方法

通讯作者: 陈焕文

English

Direct Analysis of Phospholipids in Biological Tissues Using Internal Exlractive Electrospray Ionization Mass Spectrometry

Corresponding author: 陈焕文

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs