基于激发态分子内质子转移机理的比率型荧光探针的合成及对次氯酸的检测

引用本文: 陈黎艳, 吴迪. 基于激发态分子内质子转移机理的比率型荧光探针的合成及对次氯酸的检测[J]. 分析化学, 2021, 49(8): 1350-1356. doi: 10.19756/j.issn.0253-3820.201579 shu

Citation: CHEN Li-Yan, WU Di. An Excited-state Intramolecular Proton Transfer-based Ratiometric Fluorescence Probe for Detection of Hypochlorite[J]. Chinese Journal of Analytical Chemistry, 2021, 49(8): 1350-1356. doi: 10.19756/j.issn.0253-3820.201579 shu

基于激发态分子内质子转移机理的比率型荧光探针的合成及对次氯酸的检测

陈黎艳 ^{1,
,} ,
吴迪 ^2,

1.
华中师范大学化学学院, 武汉 430079;
2.
武汉理工大学化学化工与生命科学学院, 武汉 430070

通讯作者: 陈黎艳,E-mail:clyan@mail.ccnu.edu.cn

基金项目:
国家自然科学基金项目（No.22007034）资助。

摘要: 以常见的激发态分子内质子转移（ESIPT）类型的荧光团2-（2'-羟基苯基）-苯并噻唑（HBT）为主要骨架，以高反应活性的二氨基马来腈（DMN）结构单元为识别基团，构建了比率型荧光探针HBT-CN，并对其识别性能进行了研究。HBT-CN具有良好的灵敏度，可在几秒内对HOCl做出响应，检出限低至0.11 μmol/L。干扰实验表明，探针HBT-CN不受活性氧（如H₂O₂、tBuO、ROO·）及活性氮（如NO₂^-、NO₃^-、ONOO^-、NO·）等物质的干扰，具有优异的选择性。细胞成像实验表明，探针HBT-CN具有良好的细胞膜渗透性及在活体细胞中双通道比率型检测HOCl分子的能力。

关键词:

English

An Excited-state Intramolecular Proton Transfer-based Ratiometric Fluorescence Probe for Detection of Hypochlorite

CHEN Li-Yan ^{1,
,} ,
WU Di ^2,

1.
College of Chemistry, Central China Normal University, Wuhan 430079, China;
2.
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China

Corresponding author: CHEN Li-Yan, clyan@mail.ccnu.edu.cn

Received Date: 22 September 2020
Revised Date: 25 March 2021
Fund Project:
Supported by the National Natural Science Foundation of China (No.22007034).

Abstract: A new ratiometric fluorescence probe (HBT-CN) was developed with familiar excited state intramolecular proton transfer (ESIPT) fluorophore 2-(2'-hydroxyphenyl)-benzothiazole (HBT) as framework and diaminomeleonitrile (DMN) group as reactive site. The performance of this probe to monitor HOCl was further investigated. This probe possessed good sensitivity and with the ability to rapidly detect HOCl within seconds with a detection limit as low as 1.1×10^-7 mol/L. Furthermore, this probe displayed high specificity for sensing HOCl over other relevant analytes including reactive oxygen species (H₂O₂, tBuO, ROO·) and reactive nitrogen species (NO₂^-, NO₃^-, ONOO^-, NO·). Importantly, the cell experiments demonstrated that the probe HBT-CN had good cell membrane permeability and could monitor HOCl in live cells by dual-channel.

Key words:

Benzothiazole
/ Fluorescence probe
/ Excited state intramolecular proton transfer
/ Hypochlorite
/ Cell imaging

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于激发态分子内质子转移机理的比率型荧光探针的合成及对次氯酸的检测

通讯作者: 陈黎艳,E-mail:clyan@mail.ccnu.edu.cn

English

An Excited-state Intramolecular Proton Transfer-based Ratiometric Fluorescence Probe for Detection of Hypochlorite

Corresponding author: CHEN Li-Yan, clyan@mail.ccnu.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于激发态分子内质子转移机理的比率型荧光探针的合成及对次氯酸的检测

通讯作者: 陈黎艳,E-mail:clyan@mail.ccnu.edu.cn

English

An Excited-state Intramolecular Proton Transfer-based Ratiometric Fluorescence Probe for Detection of Hypochlorite

Corresponding author: CHEN Li-Yan, clyan@mail.ccnu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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