以碳量子点为过氧化物模拟酶的葡萄糖测定方法

引用本文: 钟青梅, 黄欣虹, 覃庆敏, 苏安梅, 陈羽烨, 刘馨宇, 王益林. 以碳量子点为过氧化物模拟酶的葡萄糖测定方法[J]. 分析化学, 2018, 46(7): 1062-1068. doi: 10.11895/j.issn.0253-3820.171396 shu

Citation: ZHONG Qing-Mei, HUANG Xin-Hong, QIN Qing-Min, SU An-Mei, CHEN Yu-Ye, LIU Xin-Yu, WANG Yi-Lin. Determination of Glucose Based on Carbon Quantum Dots as Peroxidase Mimetic Enzyme[J]. Chinese Journal of Analytical Chemistry, 2018, 46(7): 1062-1068. doi: 10.11895/j.issn.0253-3820.171396 shu

以碳量子点为过氧化物模拟酶的葡萄糖测定方法

广西大学化学化工学院, 广西生物炼制重点实验室, 南宁 530004
基金项目:
本文系广西省自然科学基金项目（No.2014GXNSFAA118328）、广西生物炼制重点实验室开放基金（No.GXKLB-2）和广西大学大学生创新创业训练计划项目（No.201710593180）资助

摘要: 以烟炱为原料制备水溶性碳量子点（CQDs），此碳量子点具有过氧化物模拟酶活性，可催化H₂O₂氧化3，3'，5，5'-四甲基联苯胺（TMB）产生显色反应，而葡萄糖氧化酶（GOx）可催化葡萄糖氧化产生H₂O₂，基于上述原理，建立了一种以CQDs为过氧化物模拟酶的葡萄糖测定方法。以TMB为底物，研究了温度、pH值等条件对催化性能的影响。结果表明，pH 3.5、35℃、0.5 mmol/L TMB、1 μg/mL CQDs时，此方法检测葡萄糖的线性范围为0.025~0.40 mmol/L，检出限为5.10 μmol/L（S/N=3），尿液中的常见干扰物不干扰测定。实际尿液中的加标回收率为95.0%~105.1%。

关键词:

碳量子点
/ 模拟酶
/ 葡萄糖

English

Determination of Glucose Based on Carbon Quantum Dots as Peroxidase Mimetic Enzyme

School of Chemistry and Chemical Engineering, Guangxi University, Guangxi Key Laboratory of Biorefinery, Nanning 530004, China
Received Date: 16 November 2017
Revised Date: 11 May 2018
Fund Project:
This work was supported by the Natural Science Foundation of Guangxi Province, China (No. 2014GXNSFAA118328), the Opening Project of Guangxi Key Laboratory of Biorefinery (No. GXKLB-2) and the Foundation of College Student Experimental Skills and Innovation Ability Training of Guangxi University, China (No. 201710593180)

Abstract: Water soluble carbon quantum dots (CQDs) were prepared by using soot as carbon source. The obtained CQDs showed an excellent intrinsic peroxidase-like activity, which could catalyze the oxidization of 3,3',5,5'-tetramethylbenzidine (TMB) by H₂O₂ and thus resulted in color change. Glucose could react with dissolved oxygen to produce H₂O₂ in the presence of glucose oxidase (GOx). A colorimetric method using CQDs as peroxidase mimetic enzyme was developed for glucose determination. When TMB was acted as a substrate, the effect of a series of conditions, such as temperature and pH on the catalytic activity of the obtained CQDs, was systematically studied. Under optimal conditions, e.g. pH 3.5 and temperature 35℃, 0.5 mmol/L TMB and 1 μg/mL CQDs, the absorbance at 652 nm showed linear response with glucose concentrations ranging from 0.025 mmol/L to 0.40 mmol/L, with detection limit of 5.10 μmol/L (3σ/k). The proposed method exhibited excellent selectivity and the common substances did not interfere with detection of glucose. This method was successfully applied to detect glucose in real samples with recoveries of 95.0%-105.1%.

Key words:

1. [1]
  Song Y H, Liu H Y, Tan H L, Xu F G, Jia J B, Zhang L X, Li Z, Wang L. Anal. Chem., 2014, 86(4):1980-1987
2. [2]
  Shan D, Zhang J, Xue H G, Ding S N, Cosnier S. Biosens. Bioelectron., 2010, 25 (6):1427-1433
3. [3]
  LI Ai-Qin, GUO Chang, XU Su-Ying.Chinese J. Anal. Chem., 2017, 45(6):824-829 李爱琴, 郭唱, 许苏英. 分析化学, 2017, 45(6):824-829
4. [4]
  XU Jie, SUN Wen, HE Lu-Ying, ZHANG Qin, SU Wen-Jin. Chinese J. Anal. Chem., 2017, 45(8):1227-1232 徐杰, 孙文, 贺路影, 张芹, 苏文金. 分析化学, 2017, 45(8):1227-1232
5. [5]
  XIONG Jian, GU Cheng. Chinese J. Nanjing University (Natural Sciences), 2014, 50(4):371-387 熊键, 谷成. 南京大学学报(自然科学), 2014, 50(4):371-387
6. [6]
  Gao L Z, Zhuang J, Nie L, Zhang J B, Zhang Y, Gu N,Wang T H, Feng J, Yang D L, Perrett S, Yan X Y. Nat. Nanotechnol., 2007, 2(9):577-583
7. [7]
  Fan S S, Zhao M G, Ding L J, Li H, Chen S G. Biosens. Bioelectron., 2017, 89:846-852
8. [8]
  Mu J S, Wang Y, Zhao M, Zhang L. Chem. Commun., 2012, 48:2540-2542
9. [9]
  Bhaskar G, Tanuja B. Molecules, 2016, 21(12):1653-1669
10. [10]
  Lin L P, Song X H, Chen Y Y, Rong M C, Zhao T T, Wang Y R, Jiang Y Q, Chen X. Anal. Chim. Acta, 2015, 869:89-95
11. [11]
  Zeng Y, Miao F F, Zhao Z Y, Zhu Y T, Liu T, Chen R S, Liu S M, Lv Z S, Liang F. Appl. Sci., 2017, 7(9):924-935
12. [12]
  Cai S F, Han Q S, Qi C, Lian Z, Jia X H, Yang R, Wang C. Nanoscale, 2016, 8(6):3685-3693
13. [13]
  Jv Y, Li B X, Cao R. Chem. Commun., 2010, 46(42):8017-8019
14. [14]
  Sun W, Du Y X, Wang Y Q. Luminescence, 2010, 130(8):1463-1469
15. [15]
  Yang S T, Cao L, Luo P G, Lu F S, Wang X, Wang H F, Meziani M J, Liu Y F, Qi G, Sun Y P. J. Am. Chem. Soc., 2009, 131(32):11308-11309
16. [16]
  Wang J Q, Liu G, Leung K C, Loffroy R, Lu P X, Wang Y X. Curr. Pharm. Des., 2015, 21(37):5401-5416
17. [17]
  Wegner K D, Hildebrandt N. Chem. Soc. Rev., 2015, 44(14):4792-4834
18. [18]
  Li H T, He X D, Kang Z H, Huang H, Liu Y, Liu J L, Lian S Y, Tsang C H, Yang X B, Lee S T. Angew. Chem. Int. Edit., 2010, 49(26):4430-4434
19. [19]
  Pan J H,Zheng Z Y, Yang J Y, Wu Y Y, Lu F S, Chen Y W, Gao W H. Talanta, 2017, 166:1-7
20. [20]
  Ke J, Li X Y, Zhao Q D, Liu B J, Liu S M, Wang S B. Colloid Interface Sci., 2017, 496:425-433
21. [21]
  Li H, He X, Liu Y, Huang H,Lian S, Lee S T. Carbon, 2011, 49(2):605-609
22. [22]
  XU Yue, TANG Chun-Jing, HUANG Hong, SUN Chao-Qun, ZHANG Ya-Kun, YE Qun-Feng, WANG Ai-Jun. Chinese J. Anal. Chem., 2014, 42(9):1252-1258 胥月, 汤纯静, 黄宏, 孙超群, 张亚鲲, 叶群峰, 王爱军. 分析化学, 2014, 42(9):1252-1258
23. [23]
  HU Yue-Fang, ZUANG Liang-Liang, LIN Li-Yun, LI Xue-Feng, ZHAO Shu-Lin, LIANG Hong. Sci. Sinna Chim., 2017, 47(2):258-266 胡月芳, 张亮亮, 林丽云, 李雪凤, 赵书林, 梁宏. 中国科学:化学, 2017, 47(2):258-266
24. [24]
  Shi W B, Wang Q L, Long Y J, Cheng Z L, Chen S H, Zheng H Z, Huang Y M. Chem. Commun., 2011, 47(23):6695-6697
25. [25]
  GUO Ying, LI Wu-Wu, ZHENG Min-Yan, HUANG Yi. Acta. Chim. Sinica, 2014, 72:713-719 郭颖, 李午戊, 郑敏燕, 黄怡. 化学学报, 2014, 72:713-719
26. [26]
  Liu M, Zhao H M, Chen S. Yu H T, Quan X. ACS Nano, 2012, 3(6):3142-3151
27. [27]
  Dong Y L, Zhang H G, Rahman Z U, Su L, Chen X J, Hu J, Chen X G. Nanoscale, 2012, 4:3969-3976

扫一扫看文章

计量

PDF下载量: 11
文章访问数: 565
HTML全文浏览量: 118

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

以碳量子点为过氧化物模拟酶的葡萄糖测定方法

English

Determination of Glucose Based on Carbon Quantum Dots as Peroxidase Mimetic Enzyme

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

以碳量子点为过氧化物模拟酶的葡萄糖测定方法

English

Determination of Glucose Based on Carbon Quantum Dots as Peroxidase Mimetic Enzyme

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs