引用本文:
Nong Yue HE, Jian Xin TANG, Song LI, Hong CHEN, An Cun ZHOU. In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane[J]. Chinese Chemical Letters,
2005, 16(3): 397-400.
Citation: Nong Yue HE, Jian Xin TANG, Song LI, Hong CHEN, An Cun ZHOU. In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane[J]. Chinese Chemical Letters, 2005, 16(3): 397-400.
Citation: Nong Yue HE, Jian Xin TANG, Song LI, Hong CHEN, An Cun ZHOU. In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane[J]. Chinese Chemical Letters, 2005, 16(3): 397-400.
In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane
摘要:
Polypropylene microporous membranes were treated with plasma in a mixture of N2 and H2 (1:2 in volume). Attenuated total reflectance Fourier transform infrared spectroscopy(ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and ultra-violet (UV) spectra demonstratedthe success of grafting amino groups. The density of the polar amino groups on the membrane surface is about 0.59 μmol/cm2. The as-treated membranes were successively applied to the in situ synthesis of oligonucleotides and an average coupling yield was more than 98%. The surface feature of the treated membrane is suggested to be responsible for its advantage over a glass slide.
English
In situ Synthesis of Oligonucleotides on Plasma Treated Polypropylene Microporous Membrane
Abstract:
Polypropylene microporous membranes were treated with plasma in a mixture of N2 and H2 (1:2 in volume). Attenuated total reflectance Fourier transform infrared spectroscopy(ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and ultra-violet (UV) spectra demonstratedthe success of grafting amino groups. The density of the polar amino groups on the membrane surface is about 0.59 μmol/cm2. The as-treated membranes were successively applied to the in situ synthesis of oligonucleotides and an average coupling yield was more than 98%. The surface feature of the treated membrane is suggested to be responsible for its advantage over a glass slide.
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