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稳定同位素标记D4-罗丹明B的合成及表征

王浩然 ,  杨维成 ,  涂亚辉 ,  杨超 ,  方超 ,  李美华 ,  罗勇 ,  吴范宏

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引用本文: 王浩然, 杨维成, 涂亚辉, 杨超, 方超, 李美华, 罗勇, 吴范宏. 稳定同位素标记D4-罗丹明B的合成及表征[J]. 应用化学, 2016, 33(8): 939-944. doi: 10.11944/j.issn.1000-0518.2016.08.150374 shu
Citation:  WANG Haoran, YANG Weicheng, TU Yahui, YANG Chao, FANG Chao, LI Meihua, LUO Yong, WU Fanhong. Synthesis and Properties of Stable Isotope Deuterium Labeled D4-Rhodamine B[J]. Chinese Journal of Applied Chemistry, 2016, 33(8): 939-944. doi: 10.11944/j.issn.1000-0518.2016.08.150374 shu

稳定同位素标记D4-罗丹明B的合成及表征

  • 1.

    a 上海应用技术学院 上海 201418;

  • 2.

    b 上海化工研究院 上海 200062;

  • 3.

    c 国家同位素工程技术研究中心-上海分中心 上海 200062

    • 通讯作者: 罗勇,教授级高工; Tel/Fax:021-62652760; E-mail:real-luoyong@hotmail.com; 研究方向:精细有机合成及工业催化
  • 基金项目:

    国家科技支撑计划（2015BAK45B01-20）资助项目 

摘要: 以全氘代邻二甲苯为同位素标记前体，经氧化得到的D4-邻苯二甲酸与3-羟基-N,N-二乙基苯胺反应生成稳定同位素标记的D4-罗丹明B，总收率36.9%。目标产物的结构经质谱（MS）、核磁（NMR）等技术手段表征确认，通过高效液相色谱确定化学纯度高于98.0%，同位素氘丰度大于98.0%。将其作为同位素内标试剂用于果汁中罗丹明B的残留检测，在0.05~50 mg/L范围内呈良好的线性关系，其检出限为5 μg/kg，回收率为96.4%~103.4%，相对标准偏差（RSD）为1.0%~1.2%，具有很高的灵敏度和准确性。为食品安全领域违禁色素的检测提供了一种可靠实用的方法。

English

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    1. [1] LU Shiying,ZOU Mingqiang. Hazards Associated with the Detection of Common Non-food Pigment[J]. Chinese Instrum,2009,9(8):45-50(in Chinese).卢士英,邹明强. 食品中常见的非食用色素的危害与检测[J]. 中国仪器仪表,2009,9(8):45-50.[1] LU Shiying,ZOU Mingqiang. Hazards Associated with the Detection of Common Non-food Pigment[J]. Chinese Instrum,2009,9(8):45-50(in Chinese).卢士英,邹明强. 食品中常见的非食用色素的危害与检测[J]. 中国仪器仪表,2009,9(8):45-50.

    2. [2] Bartholomä M D,He H,Pacak C A,et al. Biological Characterization of F-18-labeled Rhodamine B, Apotential Positron Emission Tomography Perfusion Tracer[J]. Nucl Med Biol,2013,40(8):1043-1048.[2] Bartholomä M D,He H,Pacak C A,et al. Biological Characterization of F-18-labeled Rhodamine B, Apotential Positron Emission Tomography Perfusion Tracer[J]. Nucl Med Biol,2013,40(8):1043-1048.

    3. [3] Yang X F,Guo X Q,Zhao Y B. Development of a Novel Rhodamine-type Fluorescent Probe to Determine Peroxynitrite[J]. Talanta,2002,57(5):883-890.[3] Yang X F,Guo X Q,Zhao Y B. Development of a Novel Rhodamine-type Fluorescent Probe to Determine Peroxynitrite[J]. Talanta,2002,57(5):883-890.

    4. [4] CHEN Yanmei,YU Miao. Damage and Detection of Food to Human Body by Rhodamine B[J]. Friends Sci Amateurs,2011,8(9):153-153(in Chinese).陈艳美,于淼. 罗丹明 B 染色食品对人体的危害及检测[J]. 科学之友:下旬,2011,8(9):153-153.[4] CHEN Yanmei,YU Miao. Damage and Detection of Food to Human Body by Rhodamine B[J]. Friends Sci Amateurs,2011,8(9):153-153(in Chinese).陈艳美,于淼. 罗丹明 B 染色食品对人体的危害及检测[J]. 科学之友:下旬,2011,8(9):153-153.

    5. [5] Gagliardi L,De Orsi D,Cavazzutti G,et al. HPLC Determination of Rhodamine B(CI 45170) in Cosmetic Products[J]. Chromatographia,1996,43(1/2):76-78.[5] Gagliardi L,De Orsi D,Cavazzutti G,et al. HPLC Determination of Rhodamine B(CI 45170) in Cosmetic Products[J]. Chromatographia,1996,43(1/2):76-78.

    6. [6] ZHAO Shan,ZHANG Jing,DING Xiaojing,et al. Gel Purification/Ultra Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry for Detection of 11 Kinds of Azo Dyes and Rhodamine B[J]. J Instrum Anal,2012,31(4):448-452(in Chinese).赵珊,张晶,丁晓静,等. 凝胶净化/超高效液相色谱电喷雾质谱法检测调味油中11种禁用偶氮染料及罗丹明B[J]. 分析测试学报,2012,31(4):448-452.[6] ZHAO Shan,ZHANG Jing,DING Xiaojing,et al. Gel Purification/Ultra Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry for Detection of 11 Kinds of Azo Dyes and Rhodamine B[J]. J Instrum Anal,2012,31(4):448-452(in Chinese).赵珊,张晶,丁晓静,等. 凝胶净化/超高效液相色谱电喷雾质谱法检测调味油中11种禁用偶氮染料及罗丹明B[J]. 分析测试学报,2012,31(4):448-452.

    7. [7] FAN Sufang,LI Qiang,MA Junmei,et al. Improved QuEChERS Method Combines Liquid Chromatography Combined with Advanced Liquid Chromatography to Detected the Rhodamine B and Diethyl Yellow in Yuba and Tofu[J]. Chromatography,2015,33(6):657-661(in Chinese).范素芳,李强,马俊美,等. 改进的QuEChERS方法结合液相色谱-串联质谱法测定腐竹和豆干中的罗丹明B和二乙基黄[J]. 色谱,2015,33(6):657-661.[7] FAN Sufang,LI Qiang,MA Junmei,et al. Improved QuEChERS Method Combines Liquid Chromatography Combined with Advanced Liquid Chromatography to Detected the Rhodamine B and Diethyl Yellow in Yuba and Tofu[J]. Chromatography,2015,33(6):657-661(in Chinese).范素芳,李强,马俊美,等. 改进的QuEChERS方法结合液相色谱-串联质谱法测定腐竹和豆干中的罗丹明B和二乙基黄[J]. 色谱,2015,33(6):657-661.

    8. [8] Chen H,Li B,Zhan C R. HPLC-MS/MS Determination of Rhodamine B Residue in Chinese Preserved Sausages[J]. Food Sci,2010,4(31):052-053.[8] Chen H,Li B,Zhan C R. HPLC-MS/MS Determination of Rhodamine B Residue in Chinese Preserved Sausages[J]. Food Sci,2010,4(31):052-053.

    9. [9] ZHAO Rong,LI Bing,ZHAO Haiyan,et al. Solid Phase Extraction-ultra Performance Liquid Chromatography Tandem Quadrupole Mass Simultaneous Determination of 12 Industrial Dyes Spices[J]. Chinese J Food Hyg,2010,22(4):305-312(in Chinese).赵榕,李兵,赵海燕,等. 固相萃取-超高效液相色谱串接四级杆质谱同时测定调味品中12种工业染料[J]. 中国食品卫生杂志,2010,22(4):305-312.[9] ZHAO Rong,LI Bing,ZHAO Haiyan,et al. Solid Phase Extraction-ultra Performance Liquid Chromatography Tandem Quadrupole Mass Simultaneous Determination of 12 Industrial Dyes Spices[J]. Chinese J Food Hyg,2010,22(4):305-312(in Chinese).赵榕,李兵,赵海燕,等. 固相萃取-超高效液相色谱串接四级杆质谱同时测定调味品中12种工业染料[J]. 中国食品卫生杂志,2010,22(4):305-312.

    10. [10] YANG Zong,LI Xiuqin,WANG Juan,et al. Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry Accurate Determination of Chloramphenicol in Pork[J]. Chromatography,2011,29(9):927-931(in Chinese).杨总,李秀琴,王娟,等. 同位素稀释液相色谱-串联质谱法准确测定猪肉中的氯霉素[J]. 色谱,2011,29(9):927-931.[10] YANG Zong,LI Xiuqin,WANG Juan,et al. Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry Accurate Determination of Chloramphenicol in Pork[J]. Chromatography,2011,29(9):927-931(in Chinese).杨总,李秀琴,王娟,等. 同位素稀释液相色谱-串联质谱法准确测定猪肉中的氯霉素[J]. 色谱,2011,29(9):927-931.

    11. [11] FANG Liping,FENG Yuepeng,LIU haiping,et al. Isotope Dilution Gas Chromatography-mass Spectrometry in Soil/Sediment in 23 Kinds of Organochlorine Pesticides[J]. Chromatography,2010,28(5):476-482(in Chinese).房丽萍,封跃鹏,刘海萍,等. 同位素稀释气相色谱-质谱法测定土壤/底泥中23种有机氯农药[J]. 色谱,2010,28(5):476-482.[11] FANG Liping,FENG Yuepeng,LIU haiping,et al. Isotope Dilution Gas Chromatography-mass Spectrometry in Soil/Sediment in 23 Kinds of Organochlorine Pesticides[J]. Chromatography,2010,28(5):476-482(in Chinese).房丽萍,封跃鹏,刘海萍,等. 同位素稀释气相色谱-质谱法测定土壤/底泥中23种有机氯农药[J]. 色谱,2010,28(5):476-482.

    12. [12] ZHAO Motian. The Characterization of Isotope Dilution Mass Spectrometry[J]. J Chinese Mass Spectrom Soc,2004,25(10):167-168(in Chinese).赵墨田. 同位素稀释质谱法特点[J]. 质谱学报,2004,25(10):167-168.[12] ZHAO Motian. The Characterization of Isotope Dilution Mass Spectrometry[J]. J Chinese Mass Spectrom Soc,2004,25(10):167-168(in Chinese).赵墨田. 同位素稀释质谱法特点[J]. 质谱学报,2004,25(10):167-168.

    13. [13] LIU Weixia,LUO Yong,YANG Weicheng. Organic Isotope Dilution Mass Spectrometry in Food Safety Analysis Applications[J]. Chem World,2011,52(3):184-187(in Chinese).刘卫霞,罗勇,杨维成. 有机同位素稀释质谱法在食品安全分析中的应用[J]. 化学世界,2011,52(3):184-187.[13] LIU Weixia,LUO Yong,YANG Weicheng. Organic Isotope Dilution Mass Spectrometry in Food Safety Analysis Applications[J]. Chem World,2011,52(3):184-187(in Chinese).刘卫霞,罗勇,杨维成. 有机同位素稀释质谱法在食品安全分析中的应用[J]. 化学世界,2011,52(3):184-187.

    14. [14] Ku Yodugawa,Shi Osaka,Fu Osaka. Process for the Preparation of Rhodamines:EP,0468821A1[P],1991-07-26.[14] Ku Yodugawa,Shi Osaka,Fu Osaka. Process for the Preparation of Rhodamines:EP,0468821A1[P],1991-07-26.

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  • 收稿日期:  2015-10-21
  • 网络出版日期:  2016-04-25
通讯作者: 陈斌, bchen63@163.com
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