A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells

引用本文: Tun Tang, Yu-Qi Chen, Bo-Shi Fu, Zhi-Yong He, Heng Xiao, Fan Wu, Jia-Qi Wang, Shao-Ru Wang, Xiang Zhou. A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells[J]. Chinese Chemical Letters, 2016, 27(4): 540-544. shu

Citation: Tun Tang, Yu-Qi Chen, Bo-Shi Fu, Zhi-Yong He, Heng Xiao, Fan Wu, Jia-Qi Wang, Shao-Ru Wang, Xiang Zhou. A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells[J]. Chinese Chemical Letters, 2016, 27(4): 540-544. shu

A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells

College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, China
基金项目:
This work was supported by the National Basic Research Program of China (973 Program, Nos. 2012CB720600, 2012CB720603), the National Science Foundation of China (Nos. 91413109, 21202126) and East Lake High-tech Zone 3551 Talents Scheme.

摘要: In this study, a resorufin derivative RTP-1, which is a novel fluorescent "turn-on" probe for sensitive detection of hydrazine within 30 min, is designed and synthesized. The selective deprotection of the ester group of the probe by hydrazine led to a prominent enhancement of fluorescent intensity, aswell as a remarkable color change from colorless to pink, which could be distinguished by naked eye. The fluorescence enhancement showed decent linear relationship with hydrazine concentration ranging from 0 to 50 μmol/L, with a detection limit of 0.84 μmol/L. The specificity of RTP-1 for hydrazine to a number of metal ions, anions and amines is satisfactory. The sensing mechanism of RTP-1 and hydrazine was evaluated by HPLC, ESI mass spectrometry and density functional theory (DFT). Moreover, we have utilized this fluorescent probe for imaging hydrazine in living cells, and the fluorescence was clearly observed when the cells were incubated with hydrazine (100 μmol/L) for 30 min.

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English

A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells

College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, China
Received Date: 29 October 2015
Revised Date: 29 December 2015
Available Online: 22 January 2016
Fund Project:
This work was supported by the National Basic Research Program of China (973 Program, Nos. 2012CB720600, 2012CB720603), the National Science Foundation of China (Nos. 91413109, 21202126) and East Lake High-tech Zone 3551 Talents Scheme.

Abstract: In this study, a resorufin derivative RTP-1, which is a novel fluorescent "turn-on" probe for sensitive detection of hydrazine within 30 min, is designed and synthesized. The selective deprotection of the ester group of the probe by hydrazine led to a prominent enhancement of fluorescent intensity, aswell as a remarkable color change from colorless to pink, which could be distinguished by naked eye. The fluorescence enhancement showed decent linear relationship with hydrazine concentration ranging from 0 to 50 μmol/L, with a detection limit of 0.84 μmol/L. The specificity of RTP-1 for hydrazine to a number of metal ions, anions and amines is satisfactory. The sensing mechanism of RTP-1 and hydrazine was evaluated by HPLC, ESI mass spectrometry and density functional theory (DFT). Moreover, we have utilized this fluorescent probe for imaging hydrazine in living cells, and the fluorescence was clearly observed when the cells were incubated with hydrazine (100 μmol/L) for 30 min.

Key words:

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A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells

English

A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells

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通讯作者: 陈斌, bchen63@163.com

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A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells

English

A novel resorufin based fluorescent “turn-on” probe for the selective detection of hydrazine and application in living cells

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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