引用本文:
孙瑶, 吴呈珂, 李全民. 硝普钠分光光度法测定头孢他啶[J]. 应用化学,
2012, 29(9): 1082-1086.
doi:
10.3724/SP.J.1095.2012.00458
Citation: SUN Yao, WU Chengke, LI Quanmin. Spectrophotometric Determination of Ceftazidime Using Sodium Nitroprusside[J]. Chinese Journal of Applied Chemistry, 2012, 29(9): 1082-1086. doi: 10.3724/SP.J.1095.2012.00458
Citation: SUN Yao, WU Chengke, LI Quanmin. Spectrophotometric Determination of Ceftazidime Using Sodium Nitroprusside[J]. Chinese Journal of Applied Chemistry, 2012, 29(9): 1082-1086. doi: 10.3724/SP.J.1095.2012.00458
硝普钠分光光度法测定头孢他啶
English
Spectrophotometric Determination of Ceftazidime Using Sodium Nitroprusside
Abstract:
A new method is proposed to determine ceftazidime using sodium nitroprusside.In an alkaline solution,a brown product is formed by the reaction of ceftazidime with sodium nitroprusside with the stoichiometric ratio of 1:2.The amount of ceftazidime can be determined owing to the absorbance of the colored product.Absorbency is linear with the concentration of ceftazidime in the range of 2.50~810 mg/L,and the regression equation is A=0.02704+0.00218ρ(mg/L) with a correlation coefficient of 0.9987.The apparent molar absorption coefficient and the detection limit are 1.2×103 L/(mol·cm) and 1.38 mg/L,respectively.This method is simple and fast,the study indicated that the method could be applied to the determination of ceftazidime in injection with satisfactory results.
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Key words:
- sodium nitroprusside
- / ceftazidime
- / spectrophotometry
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