引用本文:
陈艳, 沈鹤柏, 徐瑞云, 朱龙章. 壳聚糖/5-氟尿嘧啶纳米粒子的制备及其性能[J]. 应用化学,
2008, 25(5): 564-568.
Citation: CHEN Yan, SHEN He-Bai, XU Rui-Yun, ZHU Long-Zhang. Preparation and Characterization of Chitosan/5-Fluorouracil Nanoparticles[J]. Chinese Journal of Applied Chemistry, 2008, 25(5): 564-568.
Citation: CHEN Yan, SHEN He-Bai, XU Rui-Yun, ZHU Long-Zhang. Preparation and Characterization of Chitosan/5-Fluorouracil Nanoparticles[J]. Chinese Journal of Applied Chemistry, 2008, 25(5): 564-568.
壳聚糖/5-氟尿嘧啶纳米粒子的制备及其性能
摘要:
采用硫酸钠和三聚磷酸钠为交联剂,通过微乳液-离子交联法制备了壳聚糖/5-氟尿嘧啶纳米粒子。所制得的粒子均呈单分散、形状规则的球形。其中用硫酸钠制备的粒子粒径为100 nm左右,载药量30%,包封率80%;用三聚磷酸钠制备的粒子粒径为40~80 nm,载药量21%,包封率55%。在pH=1的HCl溶液和pH=7.4的磷酸盐缓冲液中,进行粒子体外释药性能研究的结果表明,粒子释药量随释放时间增加而增大;相同释放时间内,粒子在磷酸盐缓冲液中的释药量明显小于在HCl溶液中的释药量。将载药粒子与K562肿瘤细胞共同培养,细胞凋亡率随着培养时间的增加而缓慢增加,表明粒子在细胞培养液中的释药量随培养时间的增加而增大,能明显促进细胞凋亡。
English
Preparation and Characterization of Chitosan/5-Fluorouracil Nanoparticles
Abstract:
Chitosan/5-fluorouracil nanoparticles (CS/5-Fu NPs) were prepared by microemulsion-ionic crosslinking method with Na2SO4 and Na5P3O10 used as crosslinkers.These NPs were monodispersed and uniformly spherical.NPs prepared by Na2SO4 were about 100 nm in size,with the drug loading efficiency (LE) and ecapsulation efficiency (EE) amounting to 30% and 80%,respectively.NPs prepared by Na5P3O10 were 40~80 nm in size,with LE and EE amounting to 21% and 55%,respectively.Sustained-release experiment was carried out in HCl solution (pH=1) and phosphate buffered solution (pH=7.4),which showed that the drug releasing amount increased with the releasing time extended,and the drug releasing performance was better in phosphate buffered solution.When cultured with the NPs,the apoptosis of K562 tumor cells was increased with the releasing time extended,which indicated that the drug releasing amount of NPs in the culture solution was increased with extended cultured time,and that the tumor cells were induced to apoptosis distinctively.
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Key words:
- chitosan
- / fluorouracil
- / microemulsion
- / ionic crosslinker
- / nanoparticle
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