引用本文:
周婕, 杜斌, 张振中. 硅胶柱层析法纯化全苯异氰酸酯基-β-环糊精[J]. 应用化学,
2008, 25(5): 548-551.
Citation: ZHOU Jie, DU Bin, ZHANG Zhen-Zhong. Separation of Mono-6-N-Allylamino-perphenylcarbamoylated β-Cyclodextrin with Silica Gel[J]. Chinese Journal of Applied Chemistry, 2008, 25(5): 548-551.
Citation: ZHOU Jie, DU Bin, ZHANG Zhen-Zhong. Separation of Mono-6-N-Allylamino-perphenylcarbamoylated β-Cyclodextrin with Silica Gel[J]. Chinese Journal of Applied Chemistry, 2008, 25(5): 548-551.
硅胶柱层析法纯化全苯异氰酸酯基-β-环糊精
English
Separation of Mono-6-N-Allylamino-perphenylcarbamoylated β-Cyclodextrin with Silica Gel
Abstract:
With the column of 0.07~0.05 mm silica gel,the separation of perphenylcarbamoylated β-cyclodextrin was investigated.The effects of mobile phase composition,bed volume,sample load,flow rate and column temperature on separation were studied systematically.The mixture was separated and the optimal conditions were obtained.The best technological conditions are approached:the bed height is 32 cm;the mobile phase is V(hexane):V(ethyl acetate)=2:1;the flow rate is 0.6 mL/min;the column temperature is 30℃;and the maximal sample load is 0.02 g.With these conditions,the recovery was 40.91% of first eluent and 50% of last eluent,respectively.
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Key words:
- cyclodextrin derivative
- / column of silica gel
- / separation
- / preparation
- / optimize
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