金纳米粒表面修饰官能团及其影响细胞作用的机制

注册 English

金纳米粒表面修饰官能团及其影响细胞作用的机制

文长春 , 雷文琪 , 沈星灿 , 纪仕辰 , 蒋邦平 , 梁宏

引用本文: 文长春, 雷文琪, 沈星灿, 纪仕辰, 蒋邦平, 梁宏. 金纳米粒表面修饰官能团及其影响细胞作用的机制[J]. 无机化学学报, 2015, 31(9): 1903-1912. doi: 10.11862/CJIC.2015.253 shu

shu

Citation: WEN Chang-Chun, LEI Wen-Qi, SHEN Xing-Can, JI Shi-Chen, JIANG Bang-Ping, LIANG Hong. Comparative Interaction Mechanisms Between Cells and Gold Nanoparticles Modified with Different Chemical Functional Groups[J]. Chinese Journal of Inorganic Chemistry, 2015, 31(9): 1903-1912. doi: 10.11862/CJIC.2015.253 shu

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金纳米粒表面修饰官能团及其影响细胞作用的机制

1.
广西师范大学药用资源化学与药物分子工程教育部重点实验室, 桂林 541004

通讯作者: 沈星灿,E-mail:xcshen@mailbox.gxnu.edu.cn;梁宏,E-mail:hliang@gxnu.edu.cn,Tel:+86-0773-5846273; 沈星灿,E-mail:xcshen@mailbox.gxnu.edu.cn;梁宏,E-mail:hliang@gxnu.edu.cn,Tel:+86-0773-5846273

基金项目:
国家自然科学基金(No.21161003,21364002) (No.21161003,21364002)

广西自然科学基金杰青(2013GXNSFGA019001) (2013GXNSFGA019001)

教育部新世纪优秀人才支持计划(NCET-13-0743) (NCET-13-0743)

药用资源化学与药物分子工程教育部重点实验室主任基金(2015-A)资助项目。 (2015-A)

摘要: 通过配体交换法,在AuNPs表面分别引入羟基(-OH),羧基(-COOH)和甲基(-CH₃),制备了3种表面修饰官能团的金纳米粒:Au-OH NPs,Au-COOH NPs和Au-CH₃ NPs,其平均粒径为(15.6±3.2)nm,ζ电位均为负值。MTT法对比研究表面修饰和未修饰的AuNPs与HeLa细胞和MCG-803细胞作用后的细胞存活率,当浓度达到197 ng·mL^-1时,表现出低细胞毒性,且顺序为:AuNPs > Au-CH₃ NPs > Au-COOH NPs≈Au-OH NPs。细胞周期研究结果发现,表面未修饰的AuNPs对细胞G2/M期活动有一定的阻滞作用。单个活细胞显微拉曼光谱原位对比研究表面修饰和未修饰的AuNPs与HeLa细胞的作用,结果表明:未修饰的AuNPs和Au-CH₃ NPs与细胞作用的主靶点可能为DNA骨架、碱基和细胞磷脂膜的极性头部,而Au-COOH NPs与Au-OH NPs对这些位点作用轻微。本研究为解释表面修饰-COOH和-OH官能团可降低AuNPs细胞毒性提供了研究证据。

关键词:

金纳米粒;表面官能团;细胞毒性;分子机制

English

Comparative Interaction Mechanisms Between Cells and Gold Nanoparticles Modified with Different Chemical Functional Groups

1.
Key Laboratory of Medicinal Chemical Resources and Molecular Engineering, Ministry of Education, Guangxi Normal University, Guilin 541004, China

Corresponding author: 沈星灿,E-mail:xcshen@mailbox.gxnu.edu.cn;梁宏,E-mail:hliang@gxnu.edu.cn,Tel:+86-0773-5846273; 沈星灿,E-mail:xcshen@mailbox.gxnu.edu.cn;梁宏,E-mail:hliang@gxnu.edu.cn,Tel:+86-0773-5846273

Received Date: 18 June 2015
Fund Project:
国家自然科学基金(No.21161003,21364002)

广西自然科学基金杰青(2013GXNSFGA019001)

教育部新世纪优秀人才支持计划(NCET-13-0743)

药用资源化学与药物分子工程教育部重点实验室主任基金(2015-A)资助项目。

Abstract: Chemical functional groups of -CH₃, -COOH and -OH have been introduced to the surface of AuNPs, separately. The AuNPs, Au-OH NPs, Au-COOH NPs and Au-CH₃ NPs are spherical with dimension of (15.6±3.2) nm, displaying negative ζ potentials. The cytotoxicity of these AuNPs has been evaluated by methylthiazoletetrazolium (MTT) assay against Hela cells and MCG-803 cells in vitro, separately. MTT data reveal that the surface unmodified AuNPs exhibit low cytotoxicity at the highest concentration of 197 ng·mL^-1 for both HeLa and MCG-803 cells in vitro. The surface modified AuNPs can further decrease the inherently cytotoxicity that follows the order AuNPs > Au-CH₃ NPs > Au-COOH NPs≈Au-OH NPs. Cell cycle analysis indicates that AuNPs cause cell cycle slightly arrest at the G₂/M phase. Micro-Raman spectra of individual living HeLa cells demonstrate that the backbone and nucleic bases of DNA as well as the polar headgroup of phospholipid in cells are the probable target binding sites of AuNPs and Au-CH₃ NPs. Whereas, the interfacial interactions are significantly reduced when cells are treated with Au-COOH NPs and Au-OH NPs. Our results on the interaction mechanisms between AuNPs and cells demonstrate that AuNPs modified with surface functional groups of -COOH or -OH can improve their cytocompatibility.

Key words:

gold nanoparticles (AuNPs);surface functional groups;cycotoxicity;molecular mechanisms

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