高效液相色谱法同时测定植物油中4种甾醇烯

引用本文: 于瑞祥, 杨瑞钰, 张欣, 方晓明, 丁卓平. 高效液相色谱法同时测定植物油中4种甾醇烯[J]. 分析化学, 2012, 40(12): 1902-1906. doi: 10.3724/SP.J.1096.2012.20610 shu

Citation: YU Rui-Xiang, YANG Rui-Yu, ZHANG Xin, FANG Xiao-Ming, DING Zhuo-Ping. Simultaneous Determination of Four Steradienes in Vegetable Oils by High Performance Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, 2012, 40(12): 1902-1906. doi: 10.3724/SP.J.1096.2012.20610 shu

高效液相色谱法同时测定植物油中4种甾醇烯

于瑞祥 ^1, ,
杨瑞钰 ^2, ,
张欣 ^1, ,
方晓明 ^{3,
,} ,
丁卓平 ^{1,
,}

通讯作者: 方晓明,丁卓平; 方晓明,丁卓平

基金项目:
本文系国家质检总局科研项目(No.2012IK184) (No.2012IK184)

上海市教育委员会重点学科建设项目(No.J50704)资助 (No.J50704)

摘要: 建立了高效液相色谱法同时测定植物油中4种甾醇烯的方法。样品用石油醚提取,硅胶柱净化,反相C₃₀柱(250 mm×4.6 mm i.d,5 μm)分离,乙腈-叔丁基甲醚(75:25,V/V)作流动相,等度洗脱,紫外检测(235 nm)。结果表明:4种甾醇烯的质量浓度在0.025-1.0 mg/L范围内;峰面积与质量浓度呈良好的线性关系,线性相关系数r²>0.999。在加标水平为0.050,0.10 和0.50 mg/kg时,4种甾醇烯的加标平均回收率为89.2%-109.8%;相对标准偏差RSD<8%;方法检出限(LOD)为0.010 mg/kg。本方法已应用于实际样品分析。

关键词:

English

Simultaneous Determination of Four Steradienes in Vegetable Oils by High Performance Liquid Chromatography

1.
¹ College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China;

Corresponding author: 方晓明,丁卓平; 方晓明,丁卓平

Received Date: 14 June 2012
Fund Project:
本文系国家质检总局科研项目(No.2012IK184)

上海市教育委员会重点学科建设项目(No.J50704)资助

Abstract: A method for the simultaneous determination of four steradienes in vegetable oils by high performance liquid chromatography (HPLC) was established. The sample was dissolved in petroleum ether and passed through a silica gel column, and the analytes were eluted with petroleum ether. The chromatographic separation was carried out on a C30 column (250 mm×4.6 mm i.d, 5 μm) using UV detection at 235 nm. Acetonitrile/tert-butyl methyl ether (75:25, V/V) was served as mobile phase in isocratic elution. Results showed good linear ranges for 4 steradienes from 0.025 mg/L to 1.0 mg/L with correlation coefficients(r²)>0.999. The mean recoveries were in the range of 89.2%-109.8% at three spiked levels of 0.050, 0.10 and 0.50 mg/kg, and the relative standard deviations (RSDs) were less than 8%. The limits of detection (LODs) were of 0.010 mg/kg. The proposed method had been applied in real sample analysis.

Key words:

1. [1]
  1 Kochhar S P. Prog. Lipid Res, 1983, 22: 161-1881 Kochhar S P. Prog. Lipid Res, 1983, 22: 161-188
2. [2]
  2 Dobarganes M C, Cert A, Dieffenbacher A. Pure Appl. Chem., 1999, 71(2): 349–3592 Dobarganes M C, Cert A, Dieffenbacher A. Pure Appl. Chem., 1999, 71(2): 349–359
3. [3]
  3 Crews C, Calvet-Sarrett R, Brereton P. Journal of the American Oil Chemists, 1997, 74 (10): 1273-12803 Crews C, Calvet-Sarrett R, Brereton P. Journal of the American Oil Chemists, 1997, 74 (10): 1273-1280
4. [4]
  4 Cert A, Moreda W. J. Chromatogr. A, 1998, 823: 291-2974 Cert A, Moreda W. J. Chromatogr. A, 1998, 823: 291-297
5. [5]
  5 Crews C, Calvet-Sarrett R, Brereton P. J. Chromatogr. A, 1999, 847: 179-1855 Crews C, Calvet-Sarrett R, Brereton P. J. Chromatogr. A, 1999, 847: 179-185
6. [6]
  6 Verleyen T, Szulcewska A, Verhe R, Dewettinck K, Huyghebaert A,De Greyt W. Food Chemistry, 2002, 78: 267-2726 Verleyen T, Szulcewska A, Verhe R, Dewettinck K, Huyghebaert A,De Greyt W. Food Chemistry, 2002, 78: 267-272
7. [7]
  7 Schulte E. Fat Sci.Technol., 1994, 96(4):124-1287 Schulte E. Fat Sci.Technol., 1994, 96(4):124-128
8. [8]
  8 Amelio M, Rizzo R, Varazini F. Journal of the American Oil Chemists, 1998, 75(4): 527-5308 Amelio M, Rizzo R, Varazini F. Journal of the American Oil Chemists, 1998, 75(4): 527-530
9. [9]
  9 Animal and Vegetable Fats and Oils-Determination of Stigmastadienes in Vegetable Oils-Part 2: Method Using High-performance Liquid Chromatography (HPLC). ISO15788-2: 20039 Animal and Vegetable Fats and Oils-Determination of Stigmastadienes in Vegetable Oils-Part 2: Method Using High-performance Liquid Chromatography (HPLC). ISO15788-2: 2003

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

高效液相色谱法同时测定植物油中4种甾醇烯

通讯作者: 方晓明,丁卓平; 方晓明,丁卓平

English

Simultaneous Determination of Four Steradienes in Vegetable Oils by High Performance Liquid Chromatography

Corresponding author: 方晓明,丁卓平; 方晓明,丁卓平

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

高效液相色谱法同时测定植物油中4种甾醇烯

通讯作者: 方晓明,丁卓平; 方晓明,丁卓平

English

Simultaneous Determination of Four Steradienes in Vegetable Oils by High Performance Liquid Chromatography

Corresponding author: 方晓明,丁卓平; 方晓明,丁卓平

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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