电感耦合等离子体质谱法分析小鼠组织中以及和DNA结合的贫铀浓度与<sup>235</sup>U/<sup>238</sup>U

引用本文: 郭志英, 梁月琴, 崔晓磊, 于水. 电感耦合等离子体质谱法分析小鼠组织中以及和DNA结合的贫铀浓度与²³⁵U/²³⁸U[J]. 分析化学, 2012, 40(12): 1865-1870. doi: 10.3724/SP.J.1096.2012.20627 shu

Citation: GUO Zhi-Ying, LIANG Yue-Qin, CUI Xiao-Lei, YU Shui. Determination of Depleted Uranium and ²³⁵U/²³⁸U of Tissues and Combined with DNA in Mouse by Inductively Coupled Plasma Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2012, 40(12): 1865-1870. doi: 10.3724/SP.J.1096.2012.20627 shu

电感耦合等离子体质谱法分析小鼠组织中以及和DNA结合的贫铀浓度与²³⁵U/²³⁸U

通讯作者: 于水

基金项目:
本文系国家科技部创新方法研究项目(No.2008IM021500) (No.2008IM021500)

国家自然科学基金项目(No.81102072) (No.81102072)

第47批博士后基金项目(No.20100471003)资助 (No.20100471003)

摘要: 在DU染毒动物模型基础上,采用ICP-MS技术同时测定各组织中铀浓度和²³⁵U/²³⁸U比值,分析DU在体内的分布和代谢规律;提取纯化肝肾组织中的基因组DNA,ICP-MS检测与DNA结合的DU浓度和²³⁵U/²³⁸U。以Pt为内标元素,补偿基体效应,组织中铀的检出限为0.0019-0.0981 μg/kg;精密度(RSD)为0.92%-2.13%,回收率在100%±10%内。ICP-MS对贫铀气溶胶中²³⁵U/²³⁸U检测结果与经典的α谱仪检测结果相吻合。DNA样品采用中性溶液稀释,DNA中铀元素检出限为0.0016 μg/kg DNA,回收率为98.3%±7.3%。样本测定结果显示,肾脏和骨骼对贫铀的蓄积能力最强,脑组织几乎不受贫铀影响,其它组织的DU蓄积能力介于二者之间。在肾、肝组织中,DU可以与DNA发生结合,与肾DNA结合更持久。组织中²³⁵U/²³⁸U随染毒铀同位素组成和浓度发生规律性改变。铀浓度与²³⁵U/²³⁸U均可以作为衡量核素污染类型和程度的敏感指标。

关键词:

English

Determination of Depleted Uranium and ²³⁵U/²³⁸U of Tissues and Combined with DNA in Mouse by Inductively Coupled Plasma Mass Spectrometry

1.
Biomedical Analysis Center, Academy of Military Medical Sciences, Beijing 100850, China

Corresponding author: 于水

Received Date: 18 June 2012
Fund Project:
本文系国家科技部创新方法研究项目(No.2008IM021500)

国家自然科学基金项目(No.81102072)

第47批博士后基金项目(No.20100471003)资助

Abstract: The BALB/c mice was exposed to deplete uranium (DU) solution (²³⁵U/²³⁸U=0.00431). Blood and the tissues of kidney, liver, spleen, cerebrum, femur, lung and testicle were collected in different time after the exposion. The uranium concentration and ²³⁵U/²³⁸U ratio in tissues were determined simultaneously by inductively coupled plasma mass spectrometry (ICP-MS), and to analyze the distribution and metabolism of DU in the body. The extracted and purified genomic DNA in kidney and liver was dialyzed to excluding the DU uncombined, and quantified by ultraviolet spectrophotometry. Then the DNA was diluted with special diluents, and the binding level of DU with genomic DNA in kidney and liver was detected by ICP-MS. Pt was confirmed as the internal standard to compensate matrix influence. The limits of detection in tissues were in the range of 0.0019 μg/kg to 0.0981 μg/kg. The precision (RSD) ranged from 0.9% to 2.1%, and the recovery was about 100%±10%. The results of ²³⁵U/²³⁸U isotopic ratio in the depleted uranium aerosols detected by ICP-MS were consistent with the results detected by α-spectrometer, and RSD=6%. The genomic DNA was diluted with neutral solution. The detection limit of uranium in DNA was 0.0016 μg/kg, and the recovery was 98.3%±7.3%. The analytical results showed that DU could be differentially accumulated in different tissues, and most of DU was accumulated in kidney and bone, and scarcely enter into the brain tissue. Especially, DU would be accumulated in bone for a long time. DU could combine with genomic DNA in kidney and liver, and the binding level of DU in kidney was relatively high and persistent. The uranium concentration in tissues had relationship to some extent with ²³⁵U/²³⁸U, and the ²³⁵U/²³⁸U isotopic ratio would be altered with uranium concentration. The uranium concentration and ²³⁵U/²³⁸U can be sensitive indicator to measure the pollution of uranium.

Key words:

Inductively coupled plasma mass spectrometry
/ Depleted uranium
/ Isotopic ratio of uranium
/ Deoxyribonucleic acid

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

电感耦合等离子体质谱法分析小鼠组织中以及和DNA结合的贫铀浓度与²³⁵U/²³⁸U

通讯作者: 于水

English

Determination of Depleted Uranium and ²³⁵U/²³⁸U of Tissues and Combined with DNA in Mouse by Inductively Coupled Plasma Mass Spectrometry

Corresponding author: 于水

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

电感耦合等离子体质谱法分析小鼠组织中以及和DNA结合的贫铀浓度与235U/238U

通讯作者: 于水

English

Determination of Depleted Uranium and 235U/238U of Tissues and Combined with DNA in Mouse by Inductively Coupled Plasma Mass Spectrometry

Corresponding author: 于水

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

电感耦合等离子体质谱法分析小鼠组织中以及和DNA结合的贫铀浓度与²³⁵U/²³⁸U

Determination of Depleted Uranium and ²³⁵U/²³⁸U of Tissues and Combined with DNA in Mouse by Inductively Coupled Plasma Mass Spectrometry

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs