基于聚锂均苯三甲酸/多壁碳纳米管复合膜的葡萄糖生物传感器的研究

引用本文: 王韬懿, 徐芬, 孙立贤, 张佳鸣, 茹慧瑛, 刘颖雅, 郑倩, 张箭. 基于聚锂均苯三甲酸/多壁碳纳米管复合膜的葡萄糖生物传感器的研究[J]. 分析化学, 2012, 40(12): 1832-1838. doi: 10.3724/SP.J.1096.2012.20569 shu

Citation: WANG Tao-Yi, XU Fen, SUN Li-Xian, ZHANG Jia-Ming, RU Hui-Ying, LIU Ying-Ya, ZHENG Qian, ZHANG Jian. Glucose Biosensor Based on Poly-Lithium Benzenetricarboxylic Acid and Multi-walled Carbon Nanotubes Composite Film[J]. Chinese Journal of Analytical Chemistry, 2012, 40(12): 1832-1838. doi: 10.3724/SP.J.1096.2012.20569 shu

基于聚锂均苯三甲酸/多壁碳纳米管复合膜的葡萄糖生物传感器的研究

王韬懿 ^1, ,
徐芬 ^{1,
,} ,
孙立贤 ^{2,
,} ,
张佳鸣 ^1, ,
茹慧瑛 ^1, ,
刘颖雅 ^2, ,
郑倩 ^1, ,
张箭 ^2,

通讯作者: 徐芬,孙立贤; 徐芬,孙立贤

基金项目:
本文系国家自然科学基金(Nos.51071081

辽宁省教育厅基金(No.L2010223)资助项目 (No.L2010223)

摘要: 利用电聚合方法在裸玻碳(GC)电极上修饰一种新型金属有机框架化合物锂均苯三甲酸(Li-BTC),并采用滴涂技术制备了Nafion/GO_x/MWNTs/poly-Li-BTC/GC葡萄糖生物传感器。利用扫描电镜分析了复合膜(含MWNTs和poly-Li-BTC) 的形貌,采用循环伏安和交流阻抗方法对修饰电极的电化学性能进行了研究。结果表明,此复合膜可增大裸玻碳电极的有效表面积、改善电极的电催化活性。利用循环伏安法和计时安培法研究了葡萄糖在Nafion/GO_x/MWNTs/poly-Li-BTC/GC电极上的电化学特性。结果表明:葡萄糖的浓度在0.02-1.56 mmol/L范围内,此修饰电极的电流响应与葡萄糖的浓度呈线性关系,其相关系数为0.9992,检出限为5.1 μmol/L(信噪比为3:1)。修饰电极的米氏常数为0.832 mmol/L,回收率为96.3%-100.3%。本研究制备的葡萄糖生物传感器具有较好的重复性、重现性、选择性与稳定性,用于葡萄糖注射液中葡萄糖含量的检测,结果满意。

关键词:

English

Glucose Biosensor Based on Poly-Lithium Benzenetricarboxylic Acid and Multi-walled Carbon Nanotubes Composite Film

1.
¹ College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, China;

Corresponding author: 徐芬,孙立贤; 徐芬,孙立贤

Received Date: 31 May 2012
Fund Project:
本文系国家自然科学基金(Nos.51071081

辽宁省教育厅基金(No.L2010223)资助项目

Abstract: A novel organic metal framework compound-lithium 1,3,5-benzenetricarboxylic acid (Li-BTC) was polymerized on the bare glassy carbon electrode by electropolymerization method. Then, Nafion/GO_x/MWNTs/poly-Li-BTC/GC electrode was fabricated by drop coating method. The morphology of the composite film containing MWNTs and poly-Li-BTC was characterized by scanning electron microscopy (SEM). The electrochemical performance of the modified electrode was investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results showed the composite film increased the effective surface area and improved electrocatalytic activity of GC electrode. The electrochemical performance of Nafion/GO_x/MWNTs/poly-Li-BTC/GC electrode for detection of glucose was investigated by cyclic voltammetry and chronoamperometry. The experiment results showed that the linear range for the detection of the glucose was from 0.02 to 1.56 mmol/L with a correlation coefficient of 0.9992, detection limit of 5.1 μmol/L (S/N=3:1). The Michaelis constant was 0.832 mmol/L and recovery rate was from 96.5% to 100.3% for the modified electrode. The prepared glucose biosensor has good repeatability, reproducibility, selectivity and stability, and can be applied to determine the glucose content in glucose injection samples.

Key words:

Glucose
/ Multi-walled carbon nanotubes
/ Glucose oxidase
/ Lithium 1,3,5-benzenetricarboxylic acid
/ Modified electrode

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沈阳化工大学材料科学与工程学院沈阳 110142

基于聚锂均苯三甲酸/多壁碳纳米管复合膜的葡萄糖生物传感器的研究

通讯作者: 徐芬,孙立贤; 徐芬,孙立贤

English

Glucose Biosensor Based on Poly-Lithium Benzenetricarboxylic Acid and Multi-walled Carbon Nanotubes Composite Film

Corresponding author: 徐芬,孙立贤; 徐芬,孙立贤

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于聚锂均苯三甲酸/多壁碳纳米管复合膜的葡萄糖生物传感器的研究

通讯作者: 徐芬,孙立贤; 徐芬,孙立贤

English

Glucose Biosensor Based on Poly-Lithium Benzenetricarboxylic Acid and Multi-walled Carbon Nanotubes Composite Film

Corresponding author: 徐芬,孙立贤; 徐芬,孙立贤

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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