快速检测Fenton反应诱导DNA损伤的电化学DNA传感器研究

引用本文: 吕翠, 康天放, 鲁理平, 熊岳. 快速检测Fenton反应诱导DNA损伤的电化学DNA传感器研究[J]. 分析化学, 2012, 40(12): 1822-1826. doi: 10.3724/SP.J.1096.2012.20303 shu

Citation: LV Cui, KANG Tian-Fang, LU Li-Ping, XIONG Yue. Electrochemical DNA Sensors for Rapid Detection of DNA Damage Induced by Fenton Reaction[J]. Chinese Journal of Analytical Chemistry, 2012, 40(12): 1822-1826. doi: 10.3724/SP.J.1096.2012.20303 shu

快速检测Fenton反应诱导DNA损伤的电化学DNA传感器研究

通讯作者: 康天放

基金项目:
本文系北京市教委科技发展基金重点项目(No.KZ201110005006) (No.KZ201110005006)

北京市自然科学基金(No.8102009) (No.8102009)

北京市人才强教深化计划——创新团队(No.PHR201007105)资助 (No.PHR201007105)

摘要: 利用带正电荷的聚二甲基二烯丙基氯化铵(PDDA)和带负电荷的小牛胸腺双链DNA(CT-dsDNA)之间的静电吸引作用,将其层层组装到玻碳电极表面,制成电化学DNA传感器,并利用电化学和原子力显微镜(AFM)方法与对DNA的组装进行了表征。以Ru(bpy)₃²⁺作为电化学催化剂,检测DNA的损伤,考察了电极在Fenton试剂中的温育时间以及Fenton试剂的浓度对DNA损伤程度的影响。实验表明,Fe²⁺与H₂O₂共存时,温育15 min即可较大程度地对DNA造成损伤,且可以检测到的Fenton试剂浓度为5.0×10^-5 mol/L FeSO₄/2.0×10^-4 mol/L H₂O₂。本方法具有灵敏度高、重现性好等优点。

关键词:

English

Electrochemical DNA Sensors for Rapid Detection of DNA Damage Induced by Fenton Reaction

1.
College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, China

Corresponding author: 康天放

Received Date: 23 March 2012
Fund Project:
本文系北京市教委科技发展基金重点项目(No.KZ201110005006)

北京市自然科学基金(No.8102009)

北京市人才强教深化计划——创新团队(No.PHR201007105)资助

Abstract: Layer-by-layer films of poly dimethyl diallyl ammonium chloride and DNA were assembled on the surface of a glassy carbon electrode to prepare DNA electrochemical sensor. The assembling of DNA was characterized by employing electrochemical methods and atomic force microscope. We used Ru(bpy)₃²⁺ as electrochemical catalyst to detect the oxidative damage of DNA in the membrane. The effects of incubation time and concentration of Fenton reagent on the damage of DNA were studied. The result showed that DNA could be damaged greatly when it was incubated in Fenton reagent for 15 min. The detectable concentration of Fenton reagent was 5.0×10^-5 mol/L FeSO₄/2.0×10^-4 mol/L H₂O₂. The electrochemical method using Ru(bpy)₃²⁺ as catalyst possesses high-sensitivity for detecting the damage of DNA.

Key words:

Fenton reaction
/ Deoxyribonucleic acid damage assembly
/ Layer-by-layer assembly
/ Deoxyribonucleic acid electrochemical sensors

1. [1]
  1 Barroso M F, De-los-Santos-Alvarez N, Lobo-Castanon M J, Miranda-Ordieres A J, Delerue -Matos C, Oliveira M B P P, Tunon-Blanco P. Biosens. Bioelectron., 2011, 26(5): 2396-24011 Barroso M F, De-los-Santos-Alvarez N, Lobo-Castanon M J, Miranda-Ordieres A J, Delerue -Matos C, Oliveira M B P P, Tunon-Blanco P. Biosens. Bioelectron., 2011, 26(5): 2396-2401
2. [2]
  2 Lucilene D M, Silvia H. Biosens. Bioelectron., 2006, 21(7): 1374-13822 Lucilene D M, Silvia H. Biosens. Bioelectron., 2006, 21(7): 1374-1382
3. [3]
  3 Pfeifer G P M. Technologies for Detection of DNA Damage and Mutations.New York Plenum Press, 19963 Pfeifer G P M. Technologies for Detection of DNA Damage and Mutations.New York Plenum Press, 1996
4. [4]
  4 Mugweru A, Wang B Q, Rusling J. Anal. Chem., 2004, 76(18): 5557-55634 Mugweru A, Wang B Q, Rusling J. Anal. Chem., 2004, 76(18): 5557-5563
5. [5]
  5 Fojta M, Stan kova A V. Talanta, 1998, 46(1): 155-1615 Fojta M, Stan kova A V. Talanta, 1998, 46(1): 155-161
6. [6]
  6 Liang M M, Guo L H. Environ. Sci. Technol., 2007, 41(2): 658-6646 Liang M M, Guo L H. Environ. Sci. Technol., 2007, 41(2): 658-664
7. [7]
  7 WANG Gui-Xiang, PAN Qian-Xiu, WANG Huai-Sheng. Chem. J. Chinese Universities, 2005, 26(10): 1812-18167 WANG Gui-Xiang, PAN Qian-Xiu, WANG Huai-Sheng. Chem. J. Chinese Universities, 2005, 26(10): 1812-1816
8. [8]
  王桂香, 潘芊秀, 王怀生. 高等学校化学学报, 2005, 26(10): 1812-1816王桂香, 潘芊秀, 王怀生. 高等学校化学学报, 2005, 26(10): 1812-1816
9. [9]
  8 Wang X L, Jiao K. Anal. Chim. Acta, 2010, 664(1): 34-398 Wang X L, Jiao K. Anal. Chim. Acta, 2010, 664(1): 34-39
10. [10]
  9 Wang X L, Yang T, Jiao K. Biosens. Bioelectron., 2009, 25(4): 668-6739 Wang X L, Yang T, Jiao K. Biosens. Bioelectron., 2009, 25(4): 668-673
11. [11]
  10 Toyokuni S, Sagripanti J-L. Free Radical Biology and Medicine, 1996, 20(6): 859-86410 Toyokuni S, Sagripanti J-L. Free Radical Biology and Medicine, 1996, 20(6): 859-864
12. [12]
  11 Chiorcea-Paquim A M,Corduneanu O, Oliveira S C B, Diculescu V C, Oliveira-Brett A M. Electrochim. Acta, 2009, 54(7): 1978-198511 Chiorcea-Paquim A M,Corduneanu O, Oliveira S C B, Diculescu V C, Oliveira-Brett A M. Electrochim. Acta, 2009, 54(7): 1978-1985
13. [13]
  12 Zu Y, Liu H Y, Zhang Y, Hu N F. Electrochim. Acta, 2009, 54(10): 2706-271212 Zu Y, Liu H Y, Zhang Y, Hu N F. Electrochim. Acta, 2009, 54(10): 2706-2712
14. [14]
  13 Zhou L P, Rusling J F. Anal. Chem., 2001, 73(20): 4780-478613 Zhou L P, Rusling J F. Anal. Chem., 2001, 73(20): 4780-4786
15. [15]
  14 LU Li-Ping, WANG Fei, KANG Tian-Fang, CHENG Shui-Yuan, WANG Yu-Ting. Chinese J. Anal. Chem., 2011, 39(3): 392-39614 LU Li-Ping, WANG Fei, KANG Tian-Fang, CHENG Shui-Yuan, WANG Yu-Ting. Chinese J. Anal. Chem., 2011, 39(3): 392-396
16. [16]
  鲁理平, 王飞, 康天放, 程水源, 王玉婷. 分析化学, 2011, 39(3): 392-396鲁理平, 王飞, 康天放, 程水源, 王玉婷. 分析化学, 2011, 39(3): 392-396
17. [17]
  15 Liu Y, Hu N F. Biosens. Bioelectron., 2009, 25(1): 185-19015 Liu Y, Hu N F. Biosens. Bioelectron., 2009, 25(1): 185-190
18. [18]
  16 Zhang J J, Wang B. Electroanalysis, 2008, 20(15), 1684-168916 Zhang J J, Wang B. Electroanalysis, 2008, 20(15), 1684-1689
19. [19]
  17 Lloyd D R, Phillips D H. Mutation Research, 1999, 424(1-2): 23-3617 Lloyd D R, Phillips D H. Mutation Research, 1999, 424(1-2): 23-36
20. [20]
  18 Diculescu V C, Paquim A, Brett A. Sensors, 2005, 5(6-10): 377-39318 Diculescu V C, Paquim A, Brett A. Sensors, 2005, 5(6-10): 377-393

扫一扫看文章

计量

PDF下载量: 0
文章访问数: 353
HTML全文浏览量: 42

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

快速检测Fenton反应诱导DNA损伤的电化学DNA传感器研究

通讯作者: 康天放

English

Electrochemical DNA Sensors for Rapid Detection of DNA Damage Induced by Fenton Reaction

Corresponding author: 康天放

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

快速检测Fenton反应诱导DNA损伤的电化学DNA传感器研究

通讯作者: 康天放

English

Electrochemical DNA Sensors for Rapid Detection of DNA Damage Induced by Fenton Reaction

Corresponding author: 康天放

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs