多肽诱导的巨型脂质体出芽和泄露行为

引用本文: 孙建波, 夏玉琼, 于秋红, 梁德海. 多肽诱导的巨型脂质体出芽和泄露行为[J]. 物理化学学报, 2015, 31(10): 1985-1990. doi: 10.3866/PKU.WHXB201508262 shu

Citation: SUN Jian-Bo, XIA Yu-Qiong, YU Qiu-Hong, LIANG De-Hai. Peptide-Induced Budding and Leakage Behavior of Giant Vesicles[J]. Acta Physico-Chimica Sinica, 2015, 31(10): 1985-1990. doi: 10.3866/PKU.WHXB201508262 shu

多肽诱导的巨型脂质体出芽和泄露行为

基金项目:
国家自然科学基金(21074005, 21174007)资助项目 (21074005, 21174007)

摘要:

细胞膜与膜蛋白之间的相互作用与生命中许多过程息息相关. 以巨型脂质体(GUV)和多肽分别作为细胞膜和膜蛋白的简化模型, 我们设计了四种仅包含亮氨酸(L)和赖氨酸(K)的多肽, 即K₁₄、(KL₂KL₂K)₂、(KL₂KL₃)₂和K₆L₈, 并对比研究了它们与中性和负电性脂质体的相互作用. 电荷密度最高的K₁₄只是涂层在脂质体表面, 不破其囊泡结构, 但能够引起负电性脂质体发生微相分离, 属建设性相互作用. 能够形成两亲性α螺旋的(KL₂KL₂K)₂和(KL₂KL₃)₂则引起脂质体发生泄露和破裂, 属破坏性作用. 但二者引起泄露的速率在中性脂质体和负电性脂质体中的结果恰好相反, 说明泄露分两步进行: 表面吸附多肽达到一定浓度, 继而对膜进行干扰. 表面活性剂型多肽K₆L₈的氨基酸组成与(KL₂KL₂K)₂相同, 但K₆L₈只是引起负电性脂质体发生泄露, 造成中性脂质体发生外出芽. 这些简单氨基酸造成的脂质体的复杂构象变化可以统一用静电和疏水相互作用在膜上的位置和强度来进行解释. 这些结论对于深入理解膜蛋白的作用机理是有帮助的.

关键词:

多肽
/ 巨型脂质体
/ 出芽
/ 泄露

English

Peptide-Induced Budding and Leakage Behavior of Giant Vesicles

1.
Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China
Received Date: 04 May 2015
Available Online: 10 October 2015
Fund Project:
国家自然科学基金(21074005, 21174007)资助项目

Abstract:

The interactions between membrane proteins and cell membranes are critical in many life processes. Giant unilamellar vesicles (GUVs) and peptides are simple but effective models of membranes and membrane proteins, respectively. Here, we designed four peptides composed of lysine (K) and leucine (L) amino acids, K₁₄, (KL₂KL₂K)₂, (KL₂KL₃)₂, and K₆L₈, and examined their interactions with neutral and negatively charged GUVs. The peptide K₁₄ has the largest charge density and is able to coat the GUV surface without damaging its structure. Whereas, leakage is observed in both neutral and charged GUVs in the presence of (KL₂KL₂K)₂ and (KL₂KL₃)₂, which can form amphiphilic α-helices in hydrophobic environments. However, the leakage rates as a function of peptide concentration are reversed for the neutral and charged GUVs. Thus, leakage occurs in two steps: absorption of peptides on the surface up to a certain level, followed by disruption of the membrane. The peptide K₆L₈ has the same chemical composition as (KL₂KL₂K)₂, but induces leakage only on negatively charged GUVs, while neutral GUVs under outward budding. Conformational changes of GUVs induced by simple peptides can be attributed to the working location (on the surface or inside the membrane), and the strength of electrostatic and hydrophobic interactions. Overall, the results provide a better understanding of membrane protein mechanisms.

Key words:

Peptide
/ Giant vesicle
/ Budding
/ Leakage

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

多肽诱导的巨型脂质体出芽和泄露行为

English

Peptide-Induced Budding and Leakage Behavior of Giant Vesicles

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

多肽诱导的巨型脂质体出芽和泄露行为

English

Peptide-Induced Budding and Leakage Behavior of Giant Vesicles

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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中国化学会秘书处

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