基于低分子量聚乙烯亚胺和油酸的负电性基因载体

引用本文: 杨阳, 郭霞. 基于低分子量聚乙烯亚胺和油酸的负电性基因载体[J]. 物理化学学报, 2014, 30(2): 345-350. doi: 10.3866/PKU.WHXB201311282 shu

Citation: YANG Yang, GUO Xia. Negatively Charged Lipopolyplex for Gene Delivery Based on Low-Molecular-Weight Polyethylenimine and Oleic Acid[J]. Acta Physico-Chimica Sinica, 2014, 30(2): 345-350. doi: 10.3866/PKU.WHXB201311282 shu

基于低分子量聚乙烯亚胺和油酸的负电性基因载体

杨阳 ^1, ,
郭霞 ^2,

基金项目:
国家自然科学基金(21073155，21373179) (21073155，21373179)

美国NIH(CA129835，CA149363，CA151652，CA129421)资助项目 (CA129835，CA149363，CA151652，CA129421)

摘要:

构建负电性的基因载体、发展基于低分子量聚乙烯亚胺(PEI)的基因载体对基因传递研究具有重要意义. 本文基于低分子量聚乙烯亚胺(2 kDa)和油酸构建了负电性的基因载体. 它通过混合聚乙烯亚胺(2 kDa)、dsDNA和油酸胶束而自发形成. 该基因载体在血清中很稳定，细胞毒性非常低，可包封80%以上DNA. 通过1，2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-N-[甲氧基(聚乙二醇2000)]铵盐(DSPE-PEG)对其表面进行修饰，发现多达90%的基因可被细胞摄取.

关键词:

聚乙烯亚胺
/ 油酸
/ 基因传递

English

Negatively Charged Lipopolyplex for Gene Delivery Based on Low-Molecular-Weight Polyethylenimine and Oleic Acid

YANG Yang ^1, ,
GUO Xia ^2,

1.
1 State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, P. R. China;
2 School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, Jiangsu Province, P. R. China
Received Date: 16 September 2013
Available Online: 23 January 2014
Fund Project:
国家自然科学基金(21073155，21373179)

美国NIH(CA129835，CA149363，CA151652，CA129421)资助项目

Abstract:

The development of synthetic vectors based on low-molecular-weight polyethylenimine (PEI) and the construction of negatively charged vectors with high nucleic acid encapsulation are two of the current research trends in gene delivery. In the present study, we developed a stable and negatively charged lipopolyplex based on oleic acid and a low-molecular-weight PEI (2 kDa); it was formed by simultaneously mixing oleic acid micelles and a polyplex produced from PEI (2 kDa) and oli dsDNA. This lipopolyplex is stable in serum, and because of the low molecular weight of the PEI, the lipopolyplex exhibits very low toxicity. The encapsulation efficiencies of nucleic acids by traditional anionic vectors are low, but very high (more than 80%) by this lipopolyplex. Suitable surface modification with 1,2-distearoryl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethyleneglycol-2000) ammonium salt enabled the lipopolyplex to bind to cells, and a high cellular uptake efficiency (ca 90%) was observed in vitro.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于低分子量聚乙烯亚胺和油酸的负电性基因载体

English

Negatively Charged Lipopolyplex for Gene Delivery Based on Low-Molecular-Weight Polyethylenimine and Oleic Acid

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于低分子量聚乙烯亚胺和油酸的负电性基因载体

English

Negatively Charged Lipopolyplex for Gene Delivery Based on Low-Molecular-Weight Polyethylenimine and Oleic Acid

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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