酸诱导拥挤条件下肌红蛋白及突变体去折叠过程

引用本文: 张玉姣, 唐乾, 曹洪玉, 郑学仿. 酸诱导拥挤条件下肌红蛋白及突变体去折叠过程[J]. 物理化学学报, 2013, 29(08): 1785-1792. doi: 10.3866/PKU.WHXB201305271 shu

Citation: ZHANG Yu-Jiao, TANG Qian, CAO Hong-Yu, ZHENG Xue-Fang. Acid-Induced Unfolding Process of Myoglobin and Its Mutant under Macromolecular Crowding Conditions[J]. Acta Physico-Chimica Sinica, 2013, 29(08): 1785-1792. doi: 10.3866/PKU.WHXB201305271 shu

酸诱导拥挤条件下肌红蛋白及突变体去折叠过程

基金项目:
国家自然科学基金(21271036, 20871024)资助项目 (21271036, 20871024)

摘要:

采用紫外-可见吸收光谱、同步荧光光谱和圆二色(CD)光谱法研究拥挤试剂葡聚糖70 (Dextran70)和聚蔗糖70 (Ficoll70)存在条件下, 酸诱导野生型肌红蛋白Mb(WT)及其突变体Mb(D60K)的去折叠过程. 结果显示: 在Dextran70 和Ficoll70 两种拥挤环境下, Mb(WT)的变性中点pH值由4.25 分别降低到3.78 与3.76, 拥挤试剂加入后增强了Mb(WT)的耐酸能力; 肌红蛋白60位天冬氨酸(Asp)突变为赖氨酸(Lys)后, 变性中点pH值由4.25 降低到4.19, 耐酸性比野生型肌红蛋白有所增强, Mb(D60K)在Dextran70 和Ficoll70 两种拥挤环境下变性中点pH值由4.19 分别降至3.74 和3.12. 以上实验说明肌红蛋白表面氨基酸突变和拥挤试剂的添加起到了稳定血红素微环境、芳香族氨基酸及二级结构和保护蛋白天然状态的作用.

关键词:

English

Acid-Induced Unfolding Process of Myoglobin and Its Mutant under Macromolecular Crowding Conditions

1.
1 School of Life Science and Biotechnology, Dalian University, Dalian 116622, Liaoning Province, P. R. China;
2 Liaoning Key Lab of Bio-organic Chemistry Dalian University, Dalian 116622, Liaoning Province, P. R. China
Received Date: 18 March 2013
Available Online: 09 July 2013
Fund Project:
国家自然科学基金(21271036, 20871024)资助项目

Abstract:

In this paper, we studied the unfolding process of myoglobin (Mb) and its mutant Mb(D60K) induced by acid under macromolecular crowding conditions, using ultraviolet-visible absorption, synchronous fluorescence, and circular dichroism (CD) spectroscopies. The spectroscopic data showed that, with the addition of Dextran70 or Ficoll70, the denaturation midpoint of Mb(WT) decreased from 4.25 to 3.78 or 3.76, respectively. In addition, the acid tolerance of Mb(WT) improved under macromolecular crowding conditions. The denaturation midpoint of Mb(D60K) was 4.19, which was a slight decrease from that of Mb (4.25). Upon addition of Dextran70 or Ficoll70, the denaturation midpoint of Mb(D60K) decreased from 4.19 to 3.74 or 3.12, respectively. The spectroscopic results illustrated that the amino acid mutant and crowding agents could stabilize the microenvironment surrounding the heme and aromatic amino acid as well as the second structure of Mb, protecting its native state.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

酸诱导拥挤条件下肌红蛋白及突变体去折叠过程

English

Acid-Induced Unfolding Process of Myoglobin and Its Mutant under Macromolecular Crowding Conditions

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

酸诱导拥挤条件下肌红蛋白及突变体去折叠过程

English

Acid-Induced Unfolding Process of Myoglobin and Its Mutant under Macromolecular Crowding Conditions

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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