寡聚酰胺为探针的电化学DNA生物传感器

引用本文: 曹瑞国, 朱斌, 刘巍, 李经建, 徐东升. 寡聚酰胺为探针的电化学DNA生物传感器[J]. 物理化学学报, 2010, 26(04): 1119-1123. doi: 10.3866/PKU.WHXB20100427 shu

Citation: CAO Rui-Guo, ZHU Bin, LIU Wei, LI Jing-Jian, XU Dong-Sheng. Electrochemical DNA Biosensor with a Polyamide Probe[J]. Acta Physico-Chimica Sinica, 2010, 26(04): 1119-1123. doi: 10.3866/PKU.WHXB20100427 shu

寡聚酰胺为探针的电化学DNA生物传感器

摘要:

DNA分子中的碱基对可以长程传递电荷, DNA分子中的碱基π堆积结构为电荷的长程传递提供了良好的通道. 电荷在DNA分子中的传递受碱基序列的影响, 利用这种性质可以构建DNA碱基错配检测的电化学传感器. 寡聚酰胺能和DNA以小沟绑定方式高亲和力地结合, 并且具有序列识别功能, 本文以带有硝基官能团的寡聚酰胺分子为电化学探针, 设计了电化学DNA生物传感器. 结果显示, 寡聚酰胺与DNA修饰电极作用后, 电化学响应显著增强, 并且可以作为检测DNA碱基错配的电化学探针分子.

关键词:

English

Electrochemical DNA Biosensor with a Polyamide Probe

1.
Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China
Received Date: 11 December 2009
Available Online: 02 April 2010

Abstract:

The base pair stack within a DNA duplex can transport charge over long distances. The π-stacked base pairs provide an effective medium for long distance charge transport in DNA molecules. The DNA-mediated charge transport is extremely sensitive to the DNA sequence. This sensitivity can be used to fabricate electrochemical DNA biosensors for single base mismatch sensing. Polyamides are DNA recognition molecules which can bind to the minor groove in DNA to form a DNA/polyamide complex with high affinity and specificity. Polyamide molecules with the nitro group are used as an electrochemically active probe for DNA biosensors. The electrochemical properties of the nitro-polyamide was investigated on a DNA self-assembly electrode. The results indicated that the electrochemical response was enhanced intensitively, and the polyamide can be used as a probe to detect a single base pair mismatch in DNA.

Key words:

Biosensor
/ Electrochemistry
/ Polyamide
/ DNA
/ Mismatch

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沈阳化工大学材料科学与工程学院沈阳 110142

寡聚酰胺为探针的电化学DNA生物传感器

English

Electrochemical DNA Biosensor with a Polyamide Probe

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寡聚酰胺为探针的电化学DNA生物传感器

English

Electrochemical DNA Biosensor with a Polyamide Probe

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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