Thermodynamic and kinetic behaviour of the energy liberation of the mitochondria isolated from Guangchong 41 and Maxie fertile lines of rice during heat treatment were studied by using a Dupont 910 differential scanning calorimeter & 9900 Computer/Thermal analyzer system over he temperature range 273.12-353.12K. The DSC curves of the rice mitochondria were obtained. All the curves manifest themselves exothermic peaks, the exothermic peak curve are of the same type on the whole, but their thermodynamic values are different from each other. The exothermic peaks can be regarded as energy liberation peaks of the rice mitochondria in the heating processes. The temperature variation of the thermodynamic values and the apparent kinetic parameters of the energy liberation of rice mitochondria were calculated. The results indicate that the energy liberation of the Guangchong 41 rice mitochondria is easier than that of the Maxie rice mitochondria, but the mechanism of the energy liberation of the Guangchong 41 rice mitochondria is more complicated than that, of the Maxie rice mitochondria in the heating process.