引用本文:
孙豳, 武巍, 洪莲, 赵新生. 单晶硅作为新型的基因芯片基底的探索[J]. 物理化学学报,
2002, 18(07): 581-584.
doi:
10.3866/PKU.WHXB20020702
Citation: Sun Bin, Wu Wei, Hong Lian, Zhao Xin-Sheng. Single Crystal Silicon Surface as a Novel Substrate of Gene-chip[J]. Acta Physico-Chimica Sinica, 2002, 18(07): 581-584. doi: 10.3866/PKU.WHXB20020702
Citation: Sun Bin, Wu Wei, Hong Lian, Zhao Xin-Sheng. Single Crystal Silicon Surface as a Novel Substrate of Gene-chip[J]. Acta Physico-Chimica Sinica, 2002, 18(07): 581-584. doi: 10.3866/PKU.WHXB20020702
单晶硅作为新型的基因芯片基底的探索
摘要:
成功地将单晶Si(111)表面光化学引入官能团的技术与水溶性碳二亚胺催化多肽合成技术结合起来,制备并表征了ss-DNA 共价修饰的Si(111)表面.进一步证明了该表面具有特异性杂交的活性和反复使用的稳定性.为制备出可靠的、可反复使用的基因芯片提供了一种新的方法.
English
Single Crystal Silicon Surface as a Novel Substrate of Gene-chip
Abstract:
Single strand oli deoxynucleotide was covalently attached to the alkyl modified silicon (111) surface through ester condensation reaction and the result was characterized by fluorescent spectra.The wafers modified by ss-oli deoxynucleotide were used as DNA probes and the reusability of the resultant wafer was investigated.Its perfect stability in target detection process may promote the wider and more efficient application of the gene chip technology.
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