引用本文:
刘慧宏, 万永清, 陈显堂, 邹国林. 固定化辣根过氧化物酶在有机/水混合溶液中的电化学[J]. 物理化学学报,
2006, 22(07): 868-872.
doi:
10.3866/PKU.WHXB20060720
Citation: LIU Hui-Hong, WAN Yong-Qing, CHEN Xian-Tang, ZOU Guo-Lin. Electrochemistry of Immobilized Horseradish Peroxidase in Organic Solvents/Water Mixture[J]. Acta Physico-Chimica Sinica, 2006, 22(07): 868-872. doi: 10.3866/PKU.WHXB20060720
Citation: LIU Hui-Hong, WAN Yong-Qing, CHEN Xian-Tang, ZOU Guo-Lin. Electrochemistry of Immobilized Horseradish Peroxidase in Organic Solvents/Water Mixture[J]. Acta Physico-Chimica Sinica, 2006, 22(07): 868-872. doi: 10.3866/PKU.WHXB20060720
固定化辣根过氧化物酶在有机/水混合溶液中的电化学
摘要:
用魔芋多糖(KGM)将辣根过氧化物酶(HRP)固定在玻碳电极(GCE)表面, 制备了HRP-KGM膜修饰电极. 在乙醇等亲水性有机溶剂与水的混合溶液中, 包埋在KGM中的HRP 可以与电极发生直接电子传递, 且能催化还原过氧化氢、氢过氧化异丙基苯、氢过氧化叔丁基、过氧化丁酮等过氧化物. HRP-KGM膜修饰电极具有较好的稳定性和重现性, 可用于这些物质的定量检测.
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关键词:
- 辣根过氧化物酶;电化学;过氧化物
English
Electrochemistry of Immobilized Horseradish Peroxidase in Organic Solvents/Water Mixture
Abstract:
Horseradish peroxidase (HRP) was immobilized on glassy carbon electrode (GCE) surface by konjac glucomannan (KGM). HRP entrapped in the KGM film underwent fast direct transfer-electron reactions in water-organic solvent mixtures. The electrocatalysis of peroxides, such as hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, and 2-butanone peroxide, by the immobilized HRP was also explored. Stability and reproducibility of the modified electrode made it possible to determine the organic peroxides.
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