引用本文:
汤厚宽, 谢昌礼, 宋昭华, 屈松生, 廖耀庭, 刘海水. 生物热动力学研究——大肠杆菌生长速率和激活能[J]. 物理化学学报,
1987, 3(02): 113-115.
doi:
10.3866/PKU.WHXB19870201
Citation: Tang Houkuan, Xie Changli, Song Zhaohua, Qu Songsheng, Liao Yaoting, Liu Haishui. STUDIES ON BIOTHERMOKINETICS MEASUEREMENT OF MULTIPLICATION RATE CONSTANTS AND ACTIVATION ENERGY OF ESCHERICHIA COLI USING MICROCALORIMETER[J]. Acta Physico-Chimica Sinica, 1987, 3(02): 113-115. doi: 10.3866/PKU.WHXB19870201
Citation: Tang Houkuan, Xie Changli, Song Zhaohua, Qu Songsheng, Liao Yaoting, Liu Haishui. STUDIES ON BIOTHERMOKINETICS MEASUEREMENT OF MULTIPLICATION RATE CONSTANTS AND ACTIVATION ENERGY OF ESCHERICHIA COLI USING MICROCALORIMETER[J]. Acta Physico-Chimica Sinica, 1987, 3(02): 113-115. doi: 10.3866/PKU.WHXB19870201
生物热动力学研究——大肠杆菌生长速率和激活能
English
STUDIES ON BIOTHERMOKINETICS MEASUEREMENT OF MULTIPLICATION RATE CONSTANTS AND ACTIVATION ENERGY OF ESCHERICHIA COLI USING MICROCALORIMETER
Abstract:
In this paper, firstly, we have been studied the multiplication curves of E. coli at exponential phase of growth at 37 ℃ using microcalorimeter, LKB 2277 Bioactivity Monitor, and calculated the multiplication rate constant of E.coli using computer, which is k=0.0397±0.0025 min~(-1). Generation time, G=17.47 minutes, has been obtained from formula G=ln2/k, which is in close agreement with liter- ature value, G=17 minutes. Secondiy, using above method, rate constants of E. coli at different temperatures at exponential of growth have been determined. Finally, according to Arrhenius equation, the activation energy of E. coli multiplication has been obtained, which is 53.09 kJ mol~(-1).
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