基于分子键裂的高频压电石英适配体生物传感器检测肌红蛋白

引用本文: 余建芳, 司士辉, 周卓, 王桢昌, 陈金华. 基于分子键裂的高频压电石英适配体生物传感器检测肌红蛋白[J]. 分析化学, 2022, 50(7): 1065-1071. doi: 10.19756/j.issn.0253-3820.210881 shu

Citation: YU Jian-Fang, SI Shi-Hui, ZHOU Zhuo, WANG Zhen-Chang, CHEN Jin-Hua. Detection of Myoglobin by High Frequency Piezoelectric Quartz Aptamer Biosensor Based on Molecular Bond Rupture Technology[J]. Chinese Journal of Analytical Chemistry, 2022, 50(7): 1065-1071. doi: 10.19756/j.issn.0253-3820.210881 shu

基于分子键裂的高频压电石英适配体生物传感器检测肌红蛋白

余建芳 ^1, ,
司士辉 ^{1,
,} ,
周卓 ^1, ,
王桢昌 ^1, ,
陈金华 ^2,

1.
中南大学化学化工学院, 长沙 410083;
2.
湖南大学化学化工学院化学生物传感与计量学国家重点实验室, 长沙 410082

通讯作者: 司士辉,E-mail:sishihui@163.com

基金项目:
国家自然科学基金项目(No.2172810)资助。

摘要: 基于调幅谐振压电石英体表面分子键裂传感技术和双适配体夹心法构建了一种检测肌红蛋白(Myo)的生物传感器。将巯基修饰的适配体Ⅰ通过Au—S键固定在石英晶体微天平(QCM)的金电极表面以特异性结合Myo,再结合适配体Ⅱ-磁珠进行质量放大。对于基频50 MHz镀金石英晶体,在谐振频率下增加激励电压峰值为8 V,结合在压电石英体表面的适配体-磁珠会被甩脱除去,发生分子键裂过程,晶体谐振频率上升,从而实现对Myo的特异性检测。在优化的实验条件下,检测Myo的线性范围为1.0~500 ng/mL,检出限(3σ)为0.38 ng/mL,实际血清样本中加标回收率为96.4%~104.0%。与常规QCM传感技术相比,本传感方法的信号获取过程简单、快速、易操作。基于分子键裂机制的QCM生物传感器具有良好的实用性和推广价值。

关键词:

English

Detection of Myoglobin by High Frequency Piezoelectric Quartz Aptamer Biosensor Based on Molecular Bond Rupture Technology

1.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;
2.
State Key Laboratory of Chemical Biosensing and Metrology, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China

Corresponding author: SI Shi-Hui, sishihui@163.com

Received Date: 07 December 2021
Revised Date: 15 April 2022
Fund Project:
Supported by the National Natural Science Foundation of China (No.2172810).

Abstract: A biosensor for detection of myoglobin based on the technology of molecular bond rupture of piezoelectric quartz crystal excited by amplitude modulation resonant and the method of double aptamer sandwich were developed. The aptamer Ⅰ was coated onto the gold electrode surface of quartz crystal microbalance (QCM) through Au-S bond, and specifically bound to myoglobin, which coupled with aptamer Ⅱ-magnetic beads to enhance the mass response. When the peak-to-peak value of the excitation voltage was increased to 8 V at the resonant frequency for 50 MHz gold-plated quartz crystal, the layer of aptamer Ⅱ-magnetic beads bonded to the surface would be removed, resulting in the occurrence of molecular bond rupture process and the increase of the resonant frequency, and the QCM aptamer biosensor with high specificity for detection of myoglobin was developed. Under the optimized conditions, the linear range of detection of myoglobin was 1.0-500 ng/mL, and the detection limit (3σ) was 0.38 ng/mL. The recoveries of myoglobin in human serum sample ranged from 96.4% to 104.0%. Compared to traditional QCM sensing technology, the sensing method established here was simple, fast, and easy to operate. Besides, the aptamer biosensor based on molecular bond rupture technology had practical and market application value.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于分子键裂的高频压电石英适配体生物传感器检测肌红蛋白

通讯作者: 司士辉,E-mail:sishihui@163.com

English

Detection of Myoglobin by High Frequency Piezoelectric Quartz Aptamer Biosensor Based on Molecular Bond Rupture Technology

Corresponding author: SI Shi-Hui, sishihui@163.com

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于分子键裂的高频压电石英适配体生物传感器检测肌红蛋白

通讯作者: 司士辉,E-mail:sishihui@163.com

English

Detection of Myoglobin by High Frequency Piezoelectric Quartz Aptamer Biosensor Based on Molecular Bond Rupture Technology

Corresponding author: SI Shi-Hui, sishihui@163.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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