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超细ZnFe2O4纳米晶与蛋白质的相互作用

李强 ,  姬晓旭 ,  钟秋 ,  黄新堂 ,  熊丽

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引用本文: 李强, 姬晓旭, 钟秋, 黄新堂, 熊丽. 超细ZnFe2O4纳米晶与蛋白质的相互作用[J]. 无机化学学报, 2013, 29(11): 2375-2381. doi: 10.3969/j.issn.1001-4861.2013.00.368 shu
Citation:  LI Qiang, JI Xiao-Xu, ZHONG Qiu, HUANG Xin-Tang, XIONG Li. Ultrafine ZnFe2O4 Nanocrystals Interacting with Proteins[J]. Chinese Journal of Inorganic Chemistry, 2013, 29(11): 2375-2381. doi: 10.3969/j.issn.1001-4861.2013.00.368 shu

超细ZnFe2O4纳米晶与蛋白质的相互作用

  • 基金项目:

    国家自然科学基金(No.51172085)资助项目。 (No.51172085)

摘要: 水热法制备了超细ZnFe2O4纳米晶,并通过高分辨透射电镜(HRTEM)、XRD和EDX技术对其进行了表征。以牛血清白蛋白(Bovine Serum Albumin,BSA)和牛血红蛋白(Hemoglobin)为模式蛋白,研究了纳米晶在不同pH条件下对蛋白质分子的吸附及其与ζ电势的关系。通过动态光散射(Dynamic Light Scattering,DLS)和傅立叶变换红外光谱(Fourier Transform Infrared,FTIR)技术分别研究了纳米晶吸附蛋白后流体力学直径的变化以及蛋白质构象的变化。结果表明,ZnFe2O4纳米晶对牛血红蛋白的吸附符合静电吸附的规律,而对BSA的吸附则不符合静电吸附的规律。纳米晶吸附牛血红蛋白后主要以单体和三聚体的形式存在,仅存在少量的团聚体,而吸附BSA后完全以团聚体的形式存在。FTIR光谱则显示纳米晶对牛血红蛋白构象的影响大于对BSA构象的影响。在合适的pH条件下,纳米晶对BSA及牛血红蛋白的吸附容量均超过380mg·g-1,有望应用于蛋白质的高效分离。

English

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    1. [1] Mahmoudi M, Lynch I, Ejtehadi M R, et al. Chem. Rev., 2011,111:5610-5637[1] Mahmoudi M, Lynch I, Ejtehadi M R, et al. Chem. Rev., 2011,111:5610-5637

    2. [2] Nel A E, Mdler L, Velegol D, et al. Nat. Mater., 2009,8:543-557[2] Nel A E, Mdler L, Velegol D, et al. Nat. Mater., 2009,8:543-557

    3. [3] Ge C C, Du J F, Zhao L, et al. PNAS, 2011,108:16968-16973[3] Ge C C, Du J F, Zhao L, et al. PNAS, 2011,108:16968-16973

    4. [4] Hu W B, Peng C, Lü M, et al. ACS Nano, 2011,5:3693-3700[4] Hu W B, Peng C, Lü M, et al. ACS Nano, 2011,5:3693-3700

    5. [5] Sun S G, Ma M H, Qiu N, et al. Colloids. Surf. B Biointerfaces, 2011,88:246-253[5] Sun S G, Ma M H, Qiu N, et al. Colloids. Surf. B Biointerfaces, 2011,88:246-253

    6. [6] DU Chong-Lei(杜崇磊), DU Wei(杜伟), WANG Bing(汪冰), et al. Chin. J. Anal. Chem.(Fenxi Huaxue), 2010,38(6):902-908[6] DU Chong-Lei(杜崇磊), DU Wei(杜伟), WANG Bing(汪冰), et al. Chin. J. Anal. Chem.(Fenxi Huaxue), 2010,38(6):902-908

    7. [7] ZHAO Zi-Lai(赵紫来), BIAN Zheng-Yun(卞征云), CHEN Lang-Xing(陈朗星), et al. Prog. Chem.(Huaxue Jinzhan), 2006,18(10):1288-1297[7] ZHAO Zi-Lai(赵紫来), BIAN Zheng-Yun(卞征云), CHEN Lang-Xing(陈朗星), et al. Prog. Chem.(Huaxue Jinzhan), 2006,18(10):1288-1297

    8. [8] HUANG Shan-Sheng(黄杉生), YIN Yue-Fen(殷月芬), WANG Ke-Min(王柯敏), et al. Chem. J. Chinese Universities (Gaodeng Xuexiao Huaxue Xuebao), 2004,25(12):2238-2241[8] HUANG Shan-Sheng(黄杉生), YIN Yue-Fen(殷月芬), WANG Ke-Min(王柯敏), et al. Chem. J. Chinese Universities (Gaodeng Xuexiao Huaxue Xuebao), 2004,25(12):2238-2241

    9. [9] HUANG Tian-Tian(黄天天), FU Yan(付雁), ZHANG Jin-Li (张金利), et al. Prog. Chem.(Huaxue Jinzhan), 2012,24(8): 1610-1622[9] HUANG Tian-Tian(黄天天), FU Yan(付雁), ZHANG Jin-Li (张金利), et al. Prog. Chem.(Huaxue Jinzhan), 2012,24(8): 1610-1622

    10. [10]ZHANG Huai(张怀), ZHANG Yun-Huai(张云怀), LI Jing (李静), et al. Prog. Chem.(Huaxue Jinzhan), 2008,20(2/3): 253-259[10]ZHANG Huai(张怀), ZHANG Yun-Huai(张云怀), LI Jing (李静), et al. Prog. Chem.(Huaxue Jinzhan), 2008,20(2/3): 253-259

    11. [11]NI Song-Bo(倪淞波), LI Yan-Bao(李延报), WANG Xiu-Mei (王秀梅). Prog. Chem.(Huaxue Jinzhan), 2011,23(1):231-245[11]NI Song-Bo(倪淞波), LI Yan-Bao(李延报), WANG Xiu-Mei (王秀梅). Prog. Chem.(Huaxue Jinzhan), 2011,23(1):231-245

    12. [12]LÜYa-Fen(吕亚芬), CAI Cheng-Xin(蔡称心). Acta Chim. Sinica(Huaxue Xuebao), 2006,64(24):2396-2402[12]LÜYa-Fen(吕亚芬), CAI Cheng-Xin(蔡称心). Acta Chim. Sinica(Huaxue Xuebao), 2006,64(24):2396-2402

    13. [13]ZOU Xue-Yan(邹雪艳), CHU Liu-Jie(褚留杰), DONG Shuo (董烁), et al. Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2012,33(7):1394-1400[13]ZOU Xue-Yan(邹雪艳), CHU Liu-Jie(褚留杰), DONG Shuo (董烁), et al. Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2012,33(7):1394-1400

    14. [14]LI Ya-Ru(李亚茹), ZHANG Xue-Lian(张雪莲), PAN Yuan-Yuan(潘园园), et al. J. Funct. Mater.(Gongneng Cailiao), 2012,43(8):1045-1048[14]LI Ya-Ru(李亚茹), ZHANG Xue-Lian(张雪莲), PAN Yuan-Yuan(潘园园), et al. J. Funct. Mater.(Gongneng Cailiao), 2012,43(8):1045-1048

    15. [15]WU Wei(吴伟), HE Quan-Guo(贺全国), CHEN Hong(陈洪). Prog. Chem.(Huaxue Jinzhan), 2008,20(2/3):265-272[15]WU Wei(吴伟), HE Quan-Guo(贺全国), CHEN Hong(陈洪). Prog. Chem.(Huaxue Jinzhan), 2008,20(2/3):265-272

    16. [16]Xu M, Li J, Iwai H, et al. Sci. Rep., 2012,2:1-6[16]Xu M, Li J, Iwai H, et al. Sci. Rep., 2012,2:1-6

    17. [17]YANG Xiao-Chao(杨小超), MO Zhi-Hong(莫志宏). Acta Chim. Sinica(Huaxue Xuebao), 2010,68(15):1549-1552[17]YANG Xiao-Chao(杨小超), MO Zhi-Hong(莫志宏). Acta Chim. Sinica(Huaxue Xuebao), 2010,68(15):1549-1552

    18. [18]ZHANG Li(张莉), TANG Xin-Feng(唐新峰), GAO Wen-Bin (高文斌). J. Inorg. Mater.(Wuji Cailiao Xuebao), 2008,23(4): 860-864[18]ZHANG Li(张莉), TANG Xin-Feng(唐新峰), GAO Wen-Bin (高文斌). J. Inorg. Mater.(Wuji Cailiao Xuebao), 2008,23(4): 860-864

    19. [19]YUAN Yuan(袁媛), HE Xiao-Xiao(何晓晓), SHI Hui(石慧), et al. Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2010,31(11):2167-2172[19]YUAN Yuan(袁媛), HE Xiao-Xiao(何晓晓), SHI Hui(石慧), et al. Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2010,31(11):2167-2172

    20. [20]Gan X, Liu T, Zhong J, et al. Chem. Bio. Chem., 2004,5: 1686-1691[20]Gan X, Liu T, Zhong J, et al. Chem. Bio. Chem., 2004,5: 1686-1691

    21. [21]Zhou H, Gan X, Liu T, et al. J. Biochem. Biophys. Methods, 2005,64:38-45[21]Zhou H, Gan X, Liu T, et al. J. Biochem. Biophys. Methods, 2005,64:38-45

    22. [22]Zhu X, Yuri I, Gan X, et al. Biosens. Bioelectron., 2007,22: 1600-1604[22]Zhu X, Yuri I, Gan X, et al. Biosens. Bioelectron., 2007,22: 1600-1604

    23. [23]Zhou H, Gan X, Liu T, et al. Bioelectrochemistry, 2006,69: 34-40[23]Zhou H, Gan X, Liu T, et al. Bioelectrochemistry, 2006,69: 34-40

    24. [24]SHAN Hong-Yan(单洪岩), LIU Dian-Jun(刘殿骏), WANG Zhen-Xin(王振新). Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2010,31(12):2372-2374[24]SHAN Hong-Yan(单洪岩), LIU Dian-Jun(刘殿骏), WANG Zhen-Xin(王振新). Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2010,31(12):2372-2374

    25. [25]MENG Jie(孟洁), YANG Man(杨曼), WANG Chao-Ying(王 超英), et al. New Carbon Mater.(Xinxing Tancailiao), 2007, 22(3):218-226[25]MENG Jie(孟洁), YANG Man(杨曼), WANG Chao-Ying(王 超英), et al. New Carbon Mater.(Xinxing Tancailiao), 2007, 22(3):218-226

    26. [26]JU Si-Ting(鞠思婷), PU Ling-Yu(朴玲钰), YANG Lei(杨磊), et al. Prog. Chem.(Huaxue Jinzhan), 2010,22(9):1767-1775[26]JU Si-Ting(鞠思婷), PU Ling-Yu(朴玲钰), YANG Lei(杨磊), et al. Prog. Chem.(Huaxue Jinzhan), 2010,22(9):1767-1775

    27. [27]GUO Xin-Lu(过馨露), ZHANG Jian(张建), WANG Wei(王 炜). Prog. Phys.(Wulixue Jinzhan), 2012,32(6):285-293[27]GUO Xin-Lu(过馨露), ZHANG Jian(张建), WANG Wei(王 炜). Prog. Phys.(Wulixue Jinzhan), 2012,32(6):285-293

    28. [28]TANG Shi-Hua(唐世华), HUANG Jian-Bin(黄建滨). Acta Chim. Sinica(Huaxue Xuebao), 2008,66(13):1534-1540[28]TANG Shi-Hua(唐世华), HUANG Jian-Bin(黄建滨). Acta Chim. Sinica(Huaxue Xuebao), 2008,66(13):1534-1540

    29. [29]SONG Wei(宋巍), CHEN Hong(陈红). Chin. Sci. Bull.(Kexue Tongbao), 2007,52(23):2701-2704[29]SONG Wei(宋巍), CHEN Hong(陈红). Chin. Sci. Bull.(Kexue Tongbao), 2007,52(23):2701-2704

    30. [30]SHI Jie(史婕), FENG Bo(冯波), LU Xiong(鲁雄), et al. J. Inorg. Mater.(Wuji Cailiao Xuebao), 2011,26(12):1329-1303[30]SHI Jie(史婕), FENG Bo(冯波), LU Xiong(鲁雄), et al. J. Inorg. Mater.(Wuji Cailiao Xuebao), 2011,26(12):1329-1303

    31. [31]LI De-Jun(李德军), YUAN Li(袁丽), YANG Ying(杨莹), et al. Sci. China. C(Zhongguo Kexue C), 2009,39(6):596-600[31]LI De-Jun(李德军), YUAN Li(袁丽), YANG Ying(杨莹), et al. Sci. China. C(Zhongguo Kexue C), 2009,39(6):596-600

    32. [32]Doane T L, Chuang C H, Hill R J, et al. Acc. Chem. Res, 2012,45:317-326[32]Doane T L, Chuang C H, Hill R J, et al. Acc. Chem. Res, 2012,45:317-326

    33. [33]Rabe M, Verdes D, Seeger S. Adv. Colloid. Interface. Sci, 2011,162:87-106[33]Rabe M, Verdes D, Seeger S. Adv. Colloid. Interface. Sci, 2011,162:87-106

    34. [34]JIANG Yong(蒋勇), SONG Wu-Lin(宋武林), XIE Chang-Sheng(谢长生), et al. Rare Met. Mater. Eng.(Xiyou Jinshu Cailiao Yu Gongcheng), 2006,35(4):617-620[34]JIANG Yong(蒋勇), SONG Wu-Lin(宋武林), XIE Chang-Sheng(谢长生), et al. Rare Met. Mater. Eng.(Xiyou Jinshu Cailiao Yu Gongcheng), 2006,35(4):617-620

    35. [35]JIAO Zheng(焦正), CHEN Feng(陈锋), LI Min-Qiang(李民 强), et al. J. Inorg. Mater.(Wuji Cailiao Xuebao), 2002,17 (2):316-320[35]JIAO Zheng(焦正), CHEN Feng(陈锋), LI Min-Qiang(李民 强), et al. J. Inorg. Mater.(Wuji Cailiao Xuebao), 2002,17 (2):316-320

    36. [36]SUN Mo-Jie(孙墨杰), HU Quan(胡全), LI Jian(李健), et al. Acta Chim. Sinica(Huaxue Xuebao), 2013,71:213-220[36]SUN Mo-Jie(孙墨杰), HU Quan(胡全), LI Jian(李健), et al. Acta Chim. Sinica(Huaxue Xuebao), 2013,71:213-220

    37. [37]Xing Z, Ju Z, Yang J, et al. Nano Res., 2012,5(7):477-485[37]Xing Z, Ju Z, Yang J, et al. Nano Res., 2012,5(7):477-485

    38. [38]Barcena C, Sra A K, Chaubey G S, et al. Chem. Comm., 2008:2224-2226[38]Barcena C, Sra A K, Chaubey G S, et al. Chem. Comm., 2008:2224-2226

    39. [39]Gao L, Wu J, Lyle S, et al. J. Phys. Chem. C, 2008,112: 17357-17361[39]Gao L, Wu J, Lyle S, et al. J. Phys. Chem. C, 2008,112: 17357-17361

    40. [40]Komarneni S, Fregeau E, Breval E, et al. J. Am. Ceram. Soc., 1988,71(1):C26-C28[40]Komarneni S, Fregeau E, Breval E, et al. J. Am. Ceram. Soc., 1988,71(1):C26-C28

    41. [41]SANG Shang-Bin(桑商斌), GU Ying-Ying(古映莹), HUANG Ke-Long(黄可龙). J. Funct. Mater.(Gongneng Cailiao), 2001, 32(1):27-29[41]SANG Shang-Bin(桑商斌), GU Ying-Ying(古映莹), HUANG Ke-Long(黄可龙). J. Funct. Mater.(Gongneng Cailiao), 2001, 32(1):27-29

    42. [42]Kumazawa H, Oki K, Cho H M, et al. Chem. Eng. Comm, 1992,115:25-33[42]Kumazawa H, Oki K, Cho H M, et al. Chem. Eng. Comm, 1992,115:25-33

    43. [43]Rezwan K, Studart A R, Voros J, et al. J. Phys. Chem. B, 2005,109:14469-14474[43]Rezwan K, Studart A R, Voros J, et al. J. Phys. Chem. B, 2005,109:14469-14474

    44. [44]Liu X, Dai Q, Austin L, et al. J. Am. Chem. Soc., 2008,130: 2780-2782[44]Liu X, Dai Q, Austin L, et al. J. Am. Chem. Soc., 2008,130: 2780-2782

    45. [45]Barth A. Biochim. Biophys. Acta, 2007,1767:1073-1101[45]Barth A. Biochim. Biophys. Acta, 2007,1767:1073-1101

    46. [46]Waldron R D. Phys. Rev., 1955,99:1727-1735[46]Waldron R D. Phys. Rev., 1955,99:1727-1735

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  • 收稿日期:  2013-05-08
  • 网络出版日期:  2013-06-20
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