介孔SiO<sub>2</sub>的改性及对诺维信漆酶的交联固定化

引用本文: 王磊, 陈诚, 田美娟, 杨宇翔, 罗健辉, 丁彬, 王平美. 介孔SiO₂的改性及对诺维信漆酶的交联固定化[J]. 无机化学学报, 2013, 29(4): 677-688. doi: 10.3969/j.issn.1001-4861.2013.00.118 shu

Citation: WANG Lei, CHEN Cheng, TIAN Mei-Juan, YANG Yu-Xiang, LUO Jian-Hui, DING Bin, WANG Ping-Mei. Modified Mesoporous Silica: Synthesis and Immobilization Function for Novozymes Laccase[J]. Chinese Journal of Inorganic Chemistry, 2013, 29(4): 677-688. doi: 10.3969/j.issn.1001-4861.2013.00.118 shu

介孔SiO₂的改性及对诺维信漆酶的交联固定化

王磊 ^1, ,
陈诚 ^1, ,
田美娟 ^1, ,
杨宇翔 ^1, ,
罗健辉 ^2, ,
丁彬 ^2, ,
王平美 ^2,

基金项目:
中国石油天然气集团公司科学研究与技术开发项目《纳米级智能化学驱油剂研制》(2011A-1001)资助项目。 (2011A-1001)

摘要: 采用十六烷基三甲基溴化铵(CTAB)为模板剂, 四乙氧基硅烷(正硅酸乙酯, TEOS)为硅源, 硝酸为催化剂来制备介孔SiO₂, 并采用后嫁接法对介孔SiO₂进行氨基化改性。利用红外光谱(IR), X射线粉末衍射(XRD), 差热-热重分析(DTA-TG), 扫描电镜(SEM), 元素分析, 微电泳法及N₂吸附-脱附方法对改性前后的产物进行表征。结果表明氨基已成功嫁接到介孔SiO₂孔道中, 改性后的介孔SiO₂有序度有所下降, 但仍为介孔材料;改性之后介孔材料的孔径、比表面积、孔体积均变小。等电点由原来的2.74变为4.75。本文还以氨基修饰的介孔SiO₂为载体, 通过交联剂戊二醛固定诺维信(Novozymes)工业级漆酶, 并采用正交设计法对固定化条件进行了优化。研究表明漆酶经固定化后, 其操作稳定性比游离酶高。

关键词:

介孔SiO₂;嫁接法;改性;固定化;酶

English

Modified Mesoporous Silica: Synthesis and Immobilization Function for Novozymes Laccase

1.
1 Department of Chemistry, East China University of Science and Techology, Shanghai 200237, China;
Received Date: 04 September 2012
Fund Project:
中国石油天然气集团公司科学研究与技术开发项目《纳米级智能化学驱油剂研制》(2011A-1001)资助项目。

Abstract: Mesoporous silica was prepared by using hexadecyl trimethyl ammonium bromide (CTAB) as the template, tetraethoxysilane (tetraethylorthosilicate, TEOS) as the silicon source and nitric acid as the hydrolysis catalyst. The obtained sample was then modified via post grafting method. The mesoporous silica before and after modification was characterized by IR, DTA-TG, XRD, SEM, elemental analysis, micro-electrophoresis and N₂ adsorption-desorption. The results show that the mesoporous silica has been modified by amino-groups, the material after modification still belongs to a family of mesoporous materials in spite of a slight decrease in the order degree. The surface area, pore size and pore volume become smaller, and the isoelectric point is decreased from 2.74 to 4.75 after the modification. The commercial Novozymes laccase is successfully immobilized in the inner surface of amino-functionalized mesoporous silica in this work by using glutaraldehyde as the crosslinking agent. The conditions of immobilization on amino-functionalized mesoporous silica were optimized by means of orthogonal experimental designs. The results show that the operational stability of the immobilized enzyme is significantly improved in comparison with the free enzyme.

Key words:

mesoporous silica;post-synthesis grafting method;modification;enzymes;immobilization

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沈阳化工大学材料科学与工程学院沈阳 110142

介孔SiO₂的改性及对诺维信漆酶的交联固定化

English

Modified Mesoporous Silica: Synthesis and Immobilization Function for Novozymes Laccase

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介孔SiO2的改性及对诺维信漆酶的交联固定化

English

Modified Mesoporous Silica: Synthesis and Immobilization Function for Novozymes Laccase

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