铁脂质体/水分配系数测定及影响因素考察

引用本文: 李妮娜, 耿丽娜, 王琳, 元琳琳, 常彦忠, 张建军. 铁脂质体/水分配系数测定及影响因素考察[J]. 物理化学学报, 2015, 31(11): 2043-2048. doi: 10.3866/PKU.WHXB201509141 shu

Citation: LI Ni-Na, GENG Li-Na, WANG Lin, YUAN Lin-Lin, CHANG Yan-Zhong, ZHANG Jian-Jun. Determination of Iron Liposome/Water Partition Coefficients and Identification of Influencing Factors[J]. Acta Physico-Chimica Sinica, 2015, 31(11): 2043-2048. doi: 10.3866/PKU.WHXB201509141 shu

铁脂质体/水分配系数测定及影响因素考察

李妮娜 ^1, ,
耿丽娜 ^{1,
,} ,
王琳 ^1, ,
元琳琳 ^1, ,
常彦忠 ^2, ,
张建军 ^2,

1.
1 河北师范大学化学与材料科学学院, 石家庄 050024;
2.
2 河北师范大学分析测试中心, 石家庄 050024

通讯作者: 耿丽娜

基金项目:
国家自然科学基金(31201305) (31201305)

河北省自然科学基金-石药集团医药联合研究基金(C2012205082)资助项目 (C2012205082)

摘要: 采用薄膜旋转蒸发-超声法分别制备了柠檬酸铁脂质体(FAC-Lip)和血红素铁脂质体(Heme-Lip). 通过测定铁脂质体的体外释放, 发现两种铁脂质体均具有缓释性, 并且Heme-Lip的缓释性比FAC-Lip的好; 采用平衡透析法测定了脂质体/水分配系数(P), 并考察影响分配系数的因素, 初步探讨了FAC和Heme与脂质体膜结合的吉布斯自由能(ΔG), 结果发现随着胆固醇含量和药脂比例的增加, P先增大后减小, 当P最大时, 药物与磷脂膜的氢键及静电作用力最大; 在透析平衡时, 计算得到了FAC-Lip和Heme-Lip中药物与脂质体膜层之间的吉布斯自由能分别为-12.7和-18.2 kJ·mol^-1.

关键词:

English

Determination of Iron Liposome/Water Partition Coefficients and Identification of Influencing Factors

1.
1 College of Chemistry and Material Science, Hebei Normal University, Shijiazhuang 050024, P. R. China;
2.
2 Testing and Analysis Center, Hebei Normal University Shijiazhuang 050024, P. R. China

Corresponding author: 耿丽娜

Received Date: 15 May 2015
Fund Project:
国家自然科学基金(31201305)

河北省自然科学基金-石药集团医药联合研究基金(C2012205082)资助项目

Abstract: Ferric citrate liposomes (FAC-Lip) and heme liposomes (Heme-Lip) were successfully prepared by a rotary-evaporated film-ultrasonication method. The release of iron liposomes were studied in vitro, and results showed that both iron liposomes had sustained-released properties, with Heme-Lip showing superior sustained-released over FAC-Lip. The liposome/water partition coefficients (P) were determined by equilibrium dialysis and the influences on P were evaluated, as well as the binding Gibbs free energy between FAC (Heme) and liposome. The results show that P initially increased and then decreased with increasing cholesterol content and the ratio of lipid to drug. The hydrogen and electrostatic interactions were largest when P was at its maximum. At the dialysis equilibrium, the binding Gibbs free energies (ΔG) of FAC-Lip and Heme-Lip were -12.7 and -18.2 kJ·mol^-1, respectively.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

铁脂质体/水分配系数测定及影响因素考察

通讯作者: 耿丽娜

English

Determination of Iron Liposome/Water Partition Coefficients and Identification of Influencing Factors

Corresponding author: 耿丽娜

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

铁脂质体/水分配系数测定及影响因素考察

通讯作者: 耿丽娜

English

Determination of Iron Liposome/Water Partition Coefficients and Identification of Influencing Factors

Corresponding author: 耿丽娜

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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