二棕榈酰磷脂酰胆碱/豆固醇脂质体液态有序相的结构性质:同步辐射X光衍射研究

引用本文: 邬瑞光, 陈琳, 尉志武. 二棕榈酰磷脂酰胆碱/豆固醇脂质体液态有序相的结构性质:同步辐射X光衍射研究[J]. 物理化学学报, 2012, 28(08): 2008-2014. doi: 10.3866/PKU.WHXB201205171 shu

Citation: WU Rui-Guang, CHEN Lin, YU Zhi-Wu. Structural Properties of the Liquid Ordered Phase of Phosphatidylcholine/Stigmasterol Liposomes: A Synchrotron X-Ray Diffraction Study[J]. Acta Physico-Chimica Sinica, 2012, 28(08): 2008-2014. doi: 10.3866/PKU.WHXB201205171 shu

二棕榈酰磷脂酰胆碱/豆固醇脂质体液态有序相的结构性质:同步辐射X光衍射研究

基金项目:
国家自然科学基金(20633080, 21133009)资助项目 (20633080, 21133009)

摘要:

用同步辐射小角和宽角X光衍射实验技术研究了由二棕榈酰磷脂酰胆碱(DPPC)和豆固醇所形成的脂质体的液态有序相的结构性质. 结果表明液态有序相的小角X光衍射d值(d-spacing)随着固醇温度和浓度的变化仅有微小的改变. 与凝胶相及液晶相的宽角X光衍射d值相比, 液态有序相的宽角X光衍射d值有更宽的变化范围, 在30到52 °C的温度范围内, 液态有序相的宽角X光衍射d值从0.422 nm变化到0.460 nm. 电子云密度计算表明液态有序相的脂双层厚度和水层厚度都要大于与之平衡共存的液晶相的脂双层厚度和水层厚度. 电子云密度计算结果还表明液态有序相的脂双层厚度随温度升高而降低. 本研究结果对于从定量的角度认识生物膜的相态及深入认识生物膜中的有序结构具有重要意义.

关键词:

English

Structural Properties of the Liquid Ordered Phase of Phosphatidylcholine/Stigmasterol Liposomes: A Synchrotron X-Ray Diffraction Study

1.
1. Key Laboratory of Bioorganic Phosphorous Chemistry & Chemical Biology of the Ministry of Education, Department of Chemistry, Beijing 100084, P. R. China;
2. School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, P. R. China
Received Date: 09 April 2012
Available Online: 10 July 2012
Fund Project:
国家自然科学基金(20633080, 21133009)资助项目

Abstract:

The properties of the liquid ordered (L_o) phase of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/stigmasterol liposomes were investigated using synchrotron small-angle and wide-angle X-ray diffraction techniques. The lamellar repeat distance of the L_o phase, derived from small-angle X-ray scattering experiments, changes slightly with sterol concentration and temperature. While the d-spacings of the acyl chains, evaluated from wide-angle X-ray scattering data, show a much broader range than those of the gel and liquid-crystal phases, varying from 0.422 to 0.460 nm when the temperature increases from 30 to 52 °C. The electron density profiles show that the bilayer thickness and the water-layer thickness of the liquid-ordered phase are larger than that of the liquid-crystal phase, and the bilayer thickness of the liquid-ordered phase decreases with increasing temperature. The results of this study are of help in understanding the phase state and the ordered structure of biomembranes.

Key words:

Liquid ordered phase
/ Lipid raft
/ Stigmasterol
/ 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine
/ X-ray diffraction
/ Small-angle scattering
/ Electron density

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

二棕榈酰磷脂酰胆碱/豆固醇脂质体液态有序相的结构性质:同步辐射X光衍射研究

English

Structural Properties of the Liquid Ordered Phase of Phosphatidylcholine/Stigmasterol Liposomes: A Synchrotron X-Ray Diffraction Study

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

二棕榈酰磷脂酰胆碱/豆固醇脂质体液态有序相的结构性质:同步辐射X光衍射研究

English

Structural Properties of the Liquid Ordered Phase of Phosphatidylcholine/Stigmasterol Liposomes: A Synchrotron X-Ray Diffraction Study

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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