分子印迹固相萃取-高效液相色谱法测定饲料中磺胺类药物

引用本文: 杜碧柏, 许杨, 黄运红, 康敏. 分子印迹固相萃取-高效液相色谱法测定饲料中磺胺类药物[J]. 分析化学, 2012, 40(12): 1871-1876. doi: 10.3724/SP.J.1096.2012.20161 shu

Citation: DU Bi-Bai, XU Yang, Huang Yun-Hong, Kang Min. Determination of Sulfonamides in Feeds by High Performance Liquid Chromatography with Molecularly Imprinted Solid Phase Extraction[J]. Chinese Journal of Analytical Chemistry, 2012, 40(12): 1871-1876. doi: 10.3724/SP.J.1096.2012.20161 shu

分子印迹固相萃取-高效液相色谱法测定饲料中磺胺类药物

杜碧柏 ^1, ,
许杨 ^, ,
黄运红 ^1,2, ,
康敏 ^1,

通讯作者: 许杨

基金项目:
本文系食品科学与技术国家重点实验室自由探索课题(No.SKLF-TS-200815)资助 (No.SKLF-TS-200815)

摘要: 建立了基于分子印迹聚合物(MIPs)为吸附剂的固相萃取技术结合高效液相色谱检测饲料中5种磺胺类药物(SAs)的方法。以磺胺嘧啶为模板,采用紫外光引发合成MIPs,以MIPs作为吸附剂制备固相萃取柱,并对上柱、淋洗和洗脱等萃取条件进行了优化。在优化的条件下,5种SAs的检出限为0.14-0.23 mg/kg;定量限为0.38-0.47 mg/kg;平均回收率在72.1%-89.3%之间;批内与批间相对标准偏差分别小于7.9%和9.2%。与碱性氧化铝柱净化相比,分子印迹固相萃取柱净化后杂质更少,选择性更好,方法的定量限更低。

关键词:

English

Determination of Sulfonamides in Feeds by High Performance Liquid Chromatography with Molecularly Imprinted Solid Phase Extraction

1.
¹ State Key Laboratory of Food Science and Technology, Sino-Germany Joint Research Institute, Nanchang University, Nanchang 330047, China;

Corresponding author: 许杨

Received Date: 22 February 2012
Fund Project:
本文系食品科学与技术国家重点实验室自由探索课题(No.SKLF-TS-200815)资助

Abstract: A method was developed for monitoring five sulfonamides (SAs) in feeds by using molecularly imprinted polymers (MIPs) as selective solid-phase extraction sorbents coupled with high performance liquid chromatography. The MIPs were firstly synthesized by ultraviolet initiated using sulfadiazine as template. Then, the molecularly printed solid phase extraction (MISPE) column was prepared, and the MIPs were used as adsorbent. Meanwhile, the procedures of loading, washing and eluting conditions were optimized. Under the optimum conditions, the detection limit range of five SAs was 0.14-0.23 mg/kg and the quantification limit range was 0.38-0.47 mg/kg. The average recoveries were in the range of 72.1%-89.3% with a batch and batch sampling relative standard deviations less than 7.9% and 9.2%, respectively. It showed that the MISPE column decreased the matrix effect and had a better selectivity and a lower limit of quantification than Alumina B column.

Key words:

Molecularly imprinted polymer
/ Solid-phase extraction
/ Sulfonamides
/ Feed
/ High performance liquid chromatography

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

分子印迹固相萃取-高效液相色谱法测定饲料中磺胺类药物

通讯作者: 许杨

English

Determination of Sulfonamides in Feeds by High Performance Liquid Chromatography with Molecularly Imprinted Solid Phase Extraction

Corresponding author: 许杨

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

分子印迹固相萃取-高效液相色谱法测定饲料中磺胺类药物

通讯作者: 许杨

English

Determination of Sulfonamides in Feeds by High Performance Liquid Chromatography with Molecularly Imprinted Solid Phase Extraction

Corresponding author: 许杨

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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